WANG Rui-hu,GUAN Xin,CHEN Xiu-ling,et al.Identification and Phylogenetic Tree Analysis of Tomato Gray Mould Pathogen[J].Northern Horticulture,2013,37(06):127-131.
番茄灰霉病菌的鉴定及系统发育树分析
- Title:
- Identification and Phylogenetic Tree Analysis of Tomato Gray Mould Pathogen
- 文章编号:
- 1001-0009(2013)06-0127-05
- Keywords:
- tomato gray mould; Botrytis cinerea; strain isolation; identification
- 分类号:
- S 436.412.1+3
- 文献标志码:
- A
- 摘要:
- 从感染番茄灰霉病的病果上分离并纯化得到菌株t08016b，其形态学特征与灰葡萄孢属极其相似。通过扩增菌株t08016b的18S rDNA及内转录间隔区（ITS）序列，对其进行分子鉴定和分类。结果表明：该菌的18S rDNA序列与Botryotinia fuckeliana（EF110887.1）的同源性为99.94%；ITS rDNA序列与Botryotinia fuckeliana（AB444949.1）的同源性为100%，无碱基差别。对菌株t08016b进行系统发育树分析，表明t08016b属于灰葡萄孢属。利用该菌对健康番茄进行侵染，番茄灰霉病的发病率可达97%，证明其具有致病力。综合形态学鉴定、分子鉴定及致病力分析，表明菌株t08016b是一株具有强致病力的灰葡萄孢菌
- Abstract:
- Strain t08016b，which morphological characteristics was very similar to the Botrytis spp.，was isolated and purified from infected tomato fruits with typical symptoms of grey mold rot.The sequences of 18S rDNA and ITS1-5.8S-ITS2 rDNA of strain t08016b were specifically amplified and sequenced using universal primer pairs NS1/NS8 and ITS4/ITS5.The results showed that the 18S sequence of t08016b had 99.94% similarity with fuckeliana fuckeliana (EF110887.1) and the ITS1-5.8S-ITS2 rDNA sequence had 100% similarity with Botryotinia fuckeliana (AB444949.1).The results of sequence alignment and neighbor-joining tree of 18S and ITS1-5.8S-ITS2 rDNA showed that t08016b was one strain of Botryotinia fuckeliana.The incidence of tomato grey mould could up to 97% after infected by strain t08016b which indicated that t08016b possess strong pathogenicity.Comprehensive morphological identification and molecular identification and pathogenicity analysis，indicated that strain t08016b was a Botrytis cinerea strain with strong pathogenicity.
参考文献/References:
[1]Prina T L.Infection strageties of botrytis cinerea and relate necrotrophic pathogens[M].Dordrecht:Kluwer Academic Publishers,2000.
[2]李保聚,朱国仁.番茄灰霉病发展症状诊断及综合防治[J].植物保护,1998,24(6):18-20. [3]吕佩珂.中国蔬菜病虫原色图谱(第三版无公害)[M].北京:农业出版社,2002. [4]魏景超.真菌鉴定手册[M].上海:上海科学技术出版社,1979. [5]陆家云.植物病原真菌学[M].北京:中国农业出版社,2001. [6]张中义.植物病原真菌学[M].北京:中国农业出版社,2001. [7]张继民.灰葡萄孢的分离方法[J].安徽机电学院学报,1998,13(2):26-30. [8]Saitou N，Nei M.The neighbor-joining method for reconstructing trees[J].Molecular Biology and Evolution,1987,4(4):406-425. [9]Kimura M.A simple method for estimating evolutionary rate of base substitution through comparative studies of nucleotide sequences[J].Journal of Molecular Evolution,1980,16:111-120. [10]张中义.中国真菌志[M].北京:科学出版社,2003. [11]何玉华,戈梅,盛下放,等.一株抗肿瘤活性的粗榧内生真菌的鉴定及其产物特性初步研究[J].生命科学研究,2007,11(3):233-237.
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备注/Memo
第一作者简介：王瑞虎（1987-），男，硕士，研究方向为植物生物技术。E-mail:wangruihu@yahoo.com.cn.