ZHANG Li,ZHENG Peng-jing.Isolated Microspore Culture of Spring-white Radish (Raphanus sativus L.var. longpinnatus Bailey)[J].Northern Horticulture,2013,37(23):31-33.
春白萝卜游离小孢子培养的研究
- Title:
- Isolated Microspore Culture of Spring-white Radish (Raphanus sativus L.var. longpinnatus Bailey)
- 文章编号:
- 1001-0009（2013）23-0031-03
- Keywords:
- radishradish FONT-SIZE: 10.5pt">radish' target="_blank" rel="external"> FONT-SIZE: 10.5pt">; microspore culture FONT-SIZE: 10.5pt">microspore culture' target="_blank" rel="external"> FONT-SIZE: 10.5pt">; cold pretreatment FONT-SIZE: 10.5pt">cold pretreatment' target="_blank" rel="external"> FONT-SIZE: 10.5pt">; activated charcoalmicrospore culture' target="_blank" rel="external"> FONT-SIZE: 10.5pt">activated charcoalmicrospore culture; cold pretreatment; activated charcoal
- 分类号:
- S 631.1
- 文献标志码:
- A
- 摘要:
- 以9个春白萝卜品种为试材，研究了基因型对春白萝卜游离小孢子成胚率及4℃低温花蕾预处理+培养基中添加活性炭对游离小孢子胚状体发生的影响，以期建立春白萝卜自交系快速纯化体系。结果表明：在7个春白萝卜基因型中诱导出了胚状体，其中“大师”的成胚率最高，达到23个胚/30个花药。单纯的4℃低温花蕾预处理和培养基中添加100 mg/L的活性炭对春白萝卜离体小孢子成胚没有明显的促进作用。而将二者结合起来可以促进春白萝卜离体小孢子培养中胚状体的发生。合适的低温预处理时间在不同品种之间存在差别。
- Abstract:
- To establish a spring-white radish microspore culture system，taking 9 spring-white radish cultivars as materials,the effects of genotype on embryoblast rate of isolaled microspone in spring-white radish and cold pretreatment for flower buds and medium added with activated charcoal on inducing microspore embryogenesis were studied.The results showed that,microspore embryogenesis were induced among which ‘Dashi’ had the highest microspore embryogenesis rate,reached 23 embryo per 30 anthers.A significant improvement of embryogenesis was achieved by using cold pretreatments and medium with 100 mg/L activated charcoal,while single treatment of cold pretreatment and charcoal had no significant effect.There were differences of suitable cold pretreatments time among different varieties.
参考文献/References:
[1] Lichter R.Efficient yield of embryoids by culture of isolated microspores of different Brassicaceae species[J].Plant Breeding，1989,103：119-123. [2] Takahata Y，Komatsu H，Kaizuma N.Microspore culture of radish (Raphanus sativus L.)：influence of genotype and culture conditions on embryogenesis[J].Plant Cell Report，1996,16：163-166.
[3] 张丽.萝卜游离小抱子培养技术初探[J].园艺学报，2004,31(5)：676-678.
[4] 陈文辉，方淑桂，曾小玲，等.萝卜游离小饱子培养研究初报[J].福建农业学报，2006,21(4)：338-341.
[5] 周志国，龚义勤，王晓武，等.不同萝卜品种游离小抱子的诱导及培养体系优化研究[J].西北植物学报，2007,27(1)：33-38.
[6] 申书兴，粱会芬，张成合.提高大白菜小孢子胚胎发生及植株获得率的几个因素研究[J].河北农业大学学报,1999,22(4)：65-68.
[7] 刘凡，莫东发.遗传背景及活性炭对白菜小孢子胚胎发生能力的影响[J].农业生物技术学报，2001,9(3)：297-300.
[8] 张德双.白菜类蔬菜小孢子培养胚胎发生及再生株基因型分离比率[J].长江蔬菜,2004(2)：38-39.
[9] 耿建峰,侯喜林.影响白菜游离小孢子培养关键因素分析[J].园艺学报，2007,34(1)：111-116.
[10]Gland A，Lichter R，Schweiger H G.Genetic and exogenous factors affecting Embryogenesis in isolated microspore cultures of Brassica napus[J].Plant Physiol，1998,132：613-617.
[11]Carlos J，Dias S.Effect of activated charcoal on Brassica oleracea microspore culture embryogenesis[J].Euphytica,1999，108：65-69.
相似文献/References:
[1]王 爽,林 多,杨延杰.萝卜芽苗菜品种筛选[J].北方园艺,2014,38(13):31.
WANG Shuang,LIN Duo,YANG Yan-jie.Screening of Radish Cultivars Suited for Sprout Vegetable Cultivation[J].Northern Horticulture,2014,38(23):31.
[2]杨金兰,郭 竞,刘艳波,等.秋露地萝卜新品种（系）比较试验[J].北方园艺,2013,37(22):39.
YANG Jin-lan,GUO Jing,LIU Yan-bo,et al.Comparative Trial of Open Field New Cultivars(Lines) of Autumn Radish[J].Northern Horticulture,2013,37(23):39.
[3]王文全,周 颖,阿依丁别克.再生水灌溉及污泥施肥对蔬菜种子发芽及幼苗生长的影响[J].北方园艺,2013,37(22):181.
WANG Wen-quan,ZHOU Ying,Ayidingbieke.Influence of Reclaimed Water Irrigation and Sludge Fertilizer on Seed Germination and Seedling Growth of Vegetables[J].Northern Horticulture,2013,37(23):181.
[4]杨延杰,林多.邻苯二甲酸对蔬菜种子萌发和幼苗生长及根系形态的影响[J].北方园艺,2013,37(13):9.
YANG Yan-jie,LIN Duo.Effects of Phthalic Acid on Seed Germination，Seedling Growth and Root Morphology of Cucumber and Radish[J].Northern Horticulture,2013,37(23):9.
[5]张晓梅,苗增建.西宁地区温室大棚秋茬萝卜新品种比较试验[J].北方园艺,2013,37(14):61.
ZHANG Xiao-mei,MIAO Zeng-jian.Variety Comparative Test of Radish New Variety in Greenhouse in Xining Region[J].Northern Horticulture,2013,37(23):61.
[6]孙丽,李贞霞,原让花,等.微波消解ICP-AES法测定萝卜肉质根矿质元素研究[J].北方园艺,2013,37(11):34.
SUN Li,LI Zhen-xia,YUAN Rang-hua,et al.Study on Determination of Mineral Elements in Fleshy Root of Radish by Microwave Digestion ICP-AES Method[J].Northern Horticulture,2013,37(23):34.
[7]袁伟玲,梅时勇,崔磊,等.萝卜主要农艺性状与肉质根产量的相关性及通径分析[J].北方园艺,2013,37(11):37.
YUAN Wei-ling,MEI Shi-yong,CUI Lei,et al.Correlation and Path Analysis of the Major Agronomic Characters of ?Radish and Fleshy Root Yield[J].Northern Horticulture,2013,37(23):37.
[8]秦成萌,林多.不同植物生长调节剂抑制反季节萝卜抽薹效应的研究[J].北方园艺,2012,36(02):23.
QIN Cheng-meng,LIN Duo.Study on Depression Effects of Anti-season Radish Cultivation with Different Plant Growth Regulators Treatments[J].Northern Horticulture,2012,36(23):23.
[9]尹立荣,管长志,陈磊,等.胡萝卜游离小孢子培养与植株再生技术研究[J].北方园艺,2012,36(15):126.
YIN Li-rong,GUAN Chang-zhi,CHEN Lei,et al.Study on Isolated Microspore Culture and Plant Regeneration of Carrot[J].Northern Horticulture,2012,36(23):126.
[10]冉茂林,彭化贤,刘独臣,等.大蒜套种萝卜对萝卜病害及品质的影响[J].北方园艺,2012,36(17):41.
RAN Mao-Lin,PENG Hua-xian,LIU Du-chen,et al.Influence on the Disease and Quality of Radish in the Garlic Interplant Radish[J].Northern Horticulture,2012,36(23):41.
备注/Memo
第一作者简介：张丽（1970-），女，博士，副研究员，研究方向为蔬菜遗传育种。E-mail:zhangli@nercv.org.
基金项目：国家科技支撑计划资助项目（2012BAD02B00）;首都现代农业育种服务平台建设资助项目（D111100001311002）。
收稿日期：2013-06-27