WANG Yu-rong,LI Feng-juan,LIU Qing-dai,et al.Study on Analysis of Genetic Relationship of 37 Main Castor Varieties by Peroxidase Isozyme[J].Northern Horticulture,2013,37(02):95-98.
利用过氧化物同工酶法分析三十七个主要蓖麻栽培品种亲缘关系的研究
- Title:
- Study on Analysis of Genetic Relationship of 37 Main Castor Varieties by Peroxidase Isozyme
- 文章编号:
- 1001-0009(2013)02-0095-04
- Keywords:
- Ricinus communis L.; peroxidase isoenzyme; cluster analysis
- 分类号:
- S 565.6
- 文献标志码:
- A
- 摘要:
- 以37份不同来源主要蓖麻栽培品种为试材，采用聚丙烯酰胺凝胶电泳技术分析比较了其过氧化物同工酶酶带特征，并对37个蓖麻栽培品种间的亲缘关系进行了聚类分析。结果表明：37个蓖麻种质的过氧化物酶谱迁移率在0.23~0.87之间，共7条谱带，不同品种的谱带数3~7条；酶带及聚类分析表明，在相似系数为0.79时，37个蓖麻栽培品种可以分为5个类群；大部分相同来源的蓖麻种质首先聚在一起，呈现一定的地域特征。
- Abstract:
- Taking 37 castor (Ricinus communis L.) varieties as materials,the peroxidase isoenzymes of them were determined using polyacrylamide gel electrophoresis technique,and genetic ralationship of 37 castor varieties were clustering analyzed.The results showed that the electrophoretic mobilities of the isoenzymes spectra were among 0.23~0.87.There were seven peroxidase-active bands observed with a range from three to seven bands among different varieties.The zymograph and cluster analysis on the isoenzyme spectra indicated that the germplasms of the 37 castor cultivated varieties could be divided into five groups based on 0.79 of similarity coefficient.The germplasm resources from geographic regions were clustered in the same group firstly.The castor germplasms showed a certain regional characteristics.
参考文献/References:
[1]孙福来.特用工业油源作物蓖麻[J].中国种业,2005(3):21-22. [2]汪多仁.蓖麻籽与蓖麻油应用进展[J].粮食与油脂,2000(2):12-14. [3]张耀奇,王恩,徐淑荣.以蓖麻籽为原料系列化工品的研究[J].内蒙古石油化工,1999,26:33-34. [4]包红霞,张春华,李金琴,等.国外蓖麻研究及产销概况[J].内蒙古农业科技,2003(4):6-8. [5]曾祥艳,王东雪,马锦林.我国蓖麻良种选育研究现状及发展策略[J].广西热带农业,2010(6):27-29. [6]郑鹭,祁建民,方平平,等.蓖麻品种遗传多样性与亲缘关系的SRAP分析[J].武汉植物研究,2010,28(1):1-6. [7]黄文霞,何觉民,朱宏波.蓖麻种质资源遗传多样性的ISSR分析[J].西北农业学报,2008,17(1):182-184,187. [8]吴昊.我国蓖麻种质资源的综合评价[J].安徽农业科学,2007,35(29):9203-9204，9212. [9]Allan G,Williams A,Rabinowicz P D,et al.Worldwide genotyping of castor bean germplasm (Ricinus communis?L.) using AFLPs and SSRs[J].Genet Resour Crop Ev,2008,55(3):365-378. [10]Pranavi B，Sitaram G，Yamini K N，et al.Development of EST-SSR markers in castor bean (Ricinus communis?L.) and their utilization for genetic purity testing of hybrids[J].Genome,2011,54(8):684-691. [11]Bajay M M，Pinheiro J B，Batista C E A，et al.Development and characterization of microsatellite markers for castor (Ricinus communis?L.)，an important oleaginous species for biodiesel production[J].Conserv Genet Res,2009,1(1):237-239. [12]Gajera B B，Kumar N，Singh A S，et al.Assessment of geneticdiversity in castor (Ricinus communis L.) using RAPD and ISSR markers[J].Ind Crops Prod,2010,32(3):491-498. [13]Qiu LJ，Yang C，Tian B，et al.Exploiting EST databases for the development and characterization of EST-SSR markers in castor bean (Ricinus communis?L.)[J].BMC Plant Biol,2010,10:278. [14]Foster J T，Allan G J，Chan A P，et al.Single nucleotide polymorphisms for assessing genetic diversity in castor bean (Ricinus communis)[J].BMC Plant Biol,2010,10:13. [15]张维强.同工酶与植物遗传育种[M].北京:北京农业大学出版社,1993:52-55. [16]张以忠,张云,邓琳琼,等.荞麦酯酶同工酶研究[J].种子,2011,30(6):38-41. [17]王琳,王林嵩,马剑敏.萝卜过氧化物酶的稳定性研究[J].河南师范大学学报(自然科学版),2004,32(3):81-83. [18]路丹,贺学礼,刘媞,等.九种绢蒿属植物过氧化物酶同工酶分析[J].生物技术通报,2008(3):241-246. [19]张新中,纪瑛.苦参和苦豆子的过氧化物酶同工酶谱分析和比较[J].植物生理学通讯,2009(7):681-683. [20]Veasey E A，Vencovsky R，Martins P S，et al.Germplasm characterization of Sesbania accessions based on isozyme analyses[J].Genet Resour Crop Ev,2002,49(5):449-462. [21]Singh A K，Mishra A，Shukla A.Genetic assessment of traits and genetic relationship in blackgram(Vigna mungo)revealed by isoenzymes[J].Biochem Genet，2009,47(7-8):471-485. [22]李培英,朱昊,孙宗玖,等.偃麦草EST和POD同工酶遗传多样性分析[J].新疆农业科学,2011,48(1):166-171. [23]阎勇,罗兴录,张兴思,等.不同供水条件下玉米耐旱生理特性比较[J].中国农学通报,2007(9):323-326. [24]王贺正,马均,李旭毅,等.水稻开花期一些生理生化特性与品种抗旱性的关系[J].中国农业科学,2007(2):399-404. [25]Wang W B，Kim Y H，Lee H S，et al.Analysis of antioxidant enzyme activity during germination of alfalfa under salt and drought stresses[J].Plant Physiol Biochem,2009,47(7):570-577. [26]Akcay U C，Ercan O，Kavas M，et al.Drought-induced oxidative damage and antioxidant responses in peanut (Arachis?hypogaea?L.) seedlings[J].Plant Growth Regul,2010,61(1):21-28. [27]程东升,潘学仁.食用菌过氧化物酶同工酶不同染色法效果比较[J].中国食用菌,1994,13(3):15-18.
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备注/Memo
第一作者简介：王玉荣（1976-），男，山东莱州人，硕士，副研究员，现主要从事食品生物技术方面的研究工作。E-mail:wangyurong@tust.edu.cn.