WANG Ya-yi,LI Song-ling,CAI Xiao-jian,et al.Isolation and Screening of Phosphorus Solubilizing Bacterial Strains in Tibetan Plateau[J].Northern Horticulture,2012,36(15):161-163.
青藏高原溶磷菌菌株的分离筛选
- Title:
- Isolation and Screening of Phosphorus Solubilizing Bacterial Strains in Tibetan Plateau
- 文章编号:
- 1001-0009(2012)15-0161-03
- Keywords:
- Tibetan plateau; P-solubilizing bacteria; dissolved phosphorus ring; ability of P-solubilizing
- 分类号:
- S 144.9
- 文献标志码:
- A
- 摘要:
- 对青海省主要耕作区农田土壤进行了溶磷菌的分离和筛选，得到具有明显溶磷圈的溶磷菌48株。通过对溶磷圈大小测定，筛选出5-1、5-2、12-7等12株菌，溶磷圈直径（D）与菌落直径（d）比值（D/d）均≥1.5。结果表明：通过摇瓶复筛，获得3株溶磷菌，可使培养液磷含量升高，均超过180 μg/mL，而pH平均降低1个单位。因此，最终确定5-2、5-3和12-7株菌为适合青海农作区推广的高效溶磷菌。
- Abstract:
- By isolation and screening of phosphorus-solubilizing bacteria in cultivated farmland soil in Qinghai Province，48 strains were obtained which had obviously dissolved phosphorus ring.By determination of the ring size，there were 12 strains such as 5-1，5-2 and 12-7 with a ratio of dissolving phosphate zone diameter to culture community zone diameter(D/d) greater than or equal to 1.5.The results showed that three strains were isolated through broth screening increasing the content of available P in bacterial solution more than 180 μg/mL in average and pH down an average of one unit.Therefore，5-2，5-3 and 12-7 were determined as the final high dissolved phosphorus bacteria suitable for Qinghai agricultural district.
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备注/Memo
第一作者简介：王亚艺（1983-），女，硕士，助理研究员，现主要从事农业微生物及肥料研究工作。E-mail：578833756@qq.com.