LYU Huiqing,XU Jiao,HE Hua,et al.Identification and Expression Analysis of Key Genes and Transcription Factors for Flavonoid Biosynthesis in Pseudostellaria heterophylla[J].Northern Horticulture,2026,(9):16-25.[doi:10.11937/bfyy.20253438]
太子参类黄酮合成关键基因及转录因子的鉴定与表达分析
- Title:
- Identification and Expression Analysis of Key Genes and Transcription Factors for Flavonoid Biosynthesis in Pseudostellaria heterophylla
- 文章编号:
- 1001-0009(2026)09-0016-10
- Keywords:
- Pseudostellaria heterophylla; full-length transcriptome; flavonoids; enzyme genes; transcription factors
- 分类号:
- R282
- 文献标志码:
- A
- 摘要:
- 以太子参的叶片、茎、韧皮部和木质部为试材，结合三代全长转录组，参考转录组的二代转录组数据重新拼接分析及各组织总黄酮含量测定，系统分析了太子参类黄酮生物合成的关键酶基因与转录调控网络，以期为太子参类黄酮生物合成的分子机制提供参考依据。结果表明：基于三代全长转录组进行重拼接显著提升从头拼接转录组质量，共获得17 863个Unigenes，注释比例提高30%以上。太子参不同组织中总黄酮含量存在显著差异，地上部分总黄酮含量表现为叶片＞茎，地下部分为韧皮部＞木质部。差异表达分析显示，地上部分共鉴定出2 357个DEGs，主要富集于光合作用等27条通路；地下部分共鉴定出1 617个DEGs，主要富集于苯丙烷类和类黄酮生物合成等5条通路。进一步筛选出47个酶基因及9个转录因子在苯丙烷和类黄酮生物合成途径呈差异表达。其中，CHI基因与9个转录因子均呈显著正相关，表明CHI基因可能是太子参类黄酮生物合成机制的关键酶基因。
- Abstract:
- Taking leaves，stems，phloem，and xylem of Pseudostellaria heterophylla as the test materials,by combining a hybrid assembly strategy (using third-generation full-length transcriptome as reference for second-generation transcriptome reassembly) and tissue-specific total flavonoid content determination，the key enzyme genes and transcriptional regulatory network involved in flavonoid biosynthesis in P.heterophylla were systematically investigated，in order to provide references for elucidating its molecular mechanism.The results showed that reassembly based on the third-generation full-length transcriptome significantly improved transcriptome quality，yielding 17 863 unigenes with annotation rate increased by over 30%.Total flavonoid content varied significantly among different tissues was in above-ground parts，leaves>stems;in below-ground parts，phloem>xylem.Differential expression analysis identified 2 357 differentially expressed genes (DEGs) in above-ground tissues，mainly enriched in 27 pathways such as photosynthesis;and 1 617 DEGs in below-ground tissues，primarily enriched in five pathways including phenylpropanoid and flavonoid biosynthesis.Further screening revealed 47 enzyme genes and nine transcription factors differentially expressed in phenylpropanoid and flavonoid biosynthesis pathways.Among them，the CHI gene showed significant positive correlations with all nine transcription factors，suggesting that CHI may serve as a key enzyme gene in the flavonoid biosynthesis mechanism of P.heterophylla.
参考文献/References:
［1］国家药典委员会.中华人民共和国药典-一部:2020年版［M］.北京:中国医药科技出版社,2020.［2］倪建成,范永飞,叶祖云.太子参化学成分、药理作用和应用的研究进展［J］.中草药,2023,54(6):1963-1977.［3］HU D J,SHAKERIAN F,ZHAO J,et al.Chemistry,pharmacology and analysis of Pseudostellaria heterophylla:A mini-review［J］.Chinese Medicine,2019,14(1):21.［4］朱三明,郑敏敏,田恬,等.植物次生代谢途径与调控研究进展［J］.植物生理学报,2023,59(12):2188-2216.［5］LIU W,FENG Y,YU S,et al.The flavonoid biosynthesis network in plants［J］.International Journal of Molecular Sciences,2021,22(23)：12824.［6］ZHAO Q,YANG J,CUI M Y,et al.The reference genome sequence of Scutellaria baicalensis provides insights into the evolution of wogonin biosynthesis［J］.Molecular Plant,2019,12(7):935-950.［7］CHU S,WANG J,CHENG H,et al.Evolutionary study of the isoflavonoid pathway based on multiple copies analysis in soybean［J］.BMC Genetics,2014,15:76.［8］刘正杰,刘运通,徐绍忠,等.紫色番茄黄烷酮3-羟化酶基因SlF3H-like克隆与表达分析［J］.北方园艺,2022(17):26-33.［9］NODA N,AIDA R,KISHIMOTO S,et al.Genetic engineering of novel bluer-colored chrysanthemums produced by accumulation of delphinidin-based anthocyanins［J］.Plant & Cell Physiology,2013,54(10):1684-1695.［10］WANG Y,CHEN J,HE G,et al.Unlocking the potential of flavonoid biosynthesis through integrated metabolic engineering［J］.Frontiers in Plant Science,2025,16:1597007.［11］DALMAN K,WIND J J,NEMESIO-GORRIZ M,et al.Overexpression of PaNAC03,a stress induced NAC gene family transcription factor in Norway spruce leads to reduced flavonol biosynthesis and aberrant embryo development［J］.BMC Plant Biology,2017,17(1):6.［12］张瑜,徐志超,季爱加,等.bZIP转录因子调控植物次生代谢产物生物合成的研究进展［J］.植物科学学报,2017,35(1):128-137.［13］韦德群.水分胁迫下太子参的响应机制研究［D］.贵阳:贵州中医药大学,2019.［14］LI J,ZHEN W,LONG D,et al.De novo sequencing and assembly analysis of the Pseudostellaria heterophylla transcriptome［J］.PLoS One,2016,11(10):e0164235.［15］HAO S,LI S,WANG J,et al.Transcriptome analysis of phycocyanin-mediated inhibitory functions on non-small cell lung cancer A549 cell growth［J］.Marine Drugs,2018,16(12)：511.［16］LI Y F,CANRIO A V M,POWER D M,et al.Ioxynil and diethylstilbestrol disrupt vascular and heart development in zebrafish［J］.Environment International,2019,124:511-520.［17］MA Q,CHEN C,ZENG Z,et al.Transcriptomic analysis between self-and cross-pollinated pistils of tea plants (Camellia sinensis)［J］.BMC Genomics,2018,19(1):289.［18］DU H,LI X,NING L,et al.RNA-Seq analysis reveals transcript diversity and active genes after common cutworm (Spodoptera litura Fabricius) attack in resistant and susceptible wild soybean lines［J］.BMC Genomics,2019,20(1):237.［19］DEWANTO V,WU X,ADOM K K,et al.Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity［J］.Journal of Agricultural and Food Chemistry,2002,50(10):3010-3014.［20］TANG D,CHEN M,HUANG X,et al.SRplot:A free online platform for data visualization and graphing［J］.PLoS One,2023,18(11):e0294236.［21］潘媛,陈大霞,宋旭红,等.基于转录组测序挖掘国槐花蕾黄酮类物质生物合成相关基因［J］.中国中药杂志,2018,43(13):2682-2689.［22］路艳,马明东,曹慧,等.水杨酸诱导下朱砂根转录组分析及三萜皂苷生物合成途径关键酶基因挖掘［J］.华北农学报,2023,38(2):106-119.［23］YANG M,YAO B,LIN R.Profiles of metabolic genes in Uncaria rhynchophylla and characterization of the critical enzyme involved in the biosynthesis of bioactive compounds-(iso)rhynchophylline［J］.Biomolecules,2022,12(12):1790.［24］王艺萌,王知斌,孙延平,等.苍术属植物中倍半萜类化合物化学结构和生物活性研究进展［J］.中草药,2021,52(1):299-309.［25］张党权,谭晓风,王晓红.查尔酮合酶与查尔酮异构酶基因特征及转基因应用［J］.中南林业科技大学学报,2007,27(2):87-91,108.［26］侯嘉铭,尹彦超,田少凯,等.过表达CHI基因提高甘草毛状根中黄酮类化合物含量的研究［J］.药学学报,2021,56(1):319-327.［27］原晓龙,李子光,陆刚,等.滇牡丹查尔酮异构酶基因的克隆及表达分析［J］.北方园艺,2020(22):79-85.［28］穆红梅,杜秀菊,张秀省,等.植物MYB转录因子调控苯丙烷类生物合成研究［J］.北方园艺,2015(24):171-174.［29］唐彩群,雷秀锦,赵艳,等.香椿R2R3-MYB转录因子基因TsMYB1克隆、表达与功能分析［J］.北方园艺,2025(13):8-17.［30］SUN Q,JIANG S,ZHANG T,et al.Apple NAC transcription factor MdNAC52 regulates biosynthesis of anthocyanin and proanthocyanidin through MdMYB9 and MdMYB11［J］.Plant Science,2019,289:110286.［31］ZHANG J,WANG Y,MAO Z,et al.Transcription factor McWRKY71 induced by ozone stress regulates anthocyanin and proanthocyanidin biosynthesis in Malus crabapple［J］.Ecotoxicology and Environmental Safety,2022,232:113274.［32］AN J P,QU F J,YAO J F,et al.The bZIP transcription factor MdHY5 regulates anthocyanin accumulation and nitrate assimilation in apple［J］.Horticulture Research,2017,4:17023.［33］XU W,DUBOS C,LEPINIEC L.Transcriptional control of flavonoid biosynthesis by MYB-bHLH-WDR complexes［J］.Trends in Plant Science,2015,20(3):176-185.
相似文献/References:
[1]梁玉勇,廖玲,左北梅,等.太子参不定根诱导及增殖培养研究[J].北方园艺,2013,37(02):147.
LIANG Yu-yong,LIAO Ling,ZUO Bei-mei,et al.Study on Adventitious Roots Induction and Proliferation Culture of Pseudostellaria heterophylla[J].Northern Horticulture,2013,37(9):147.
[2]任永权,杨芩,徐元江,等.太子参水浸液对其种子萌发和幼苗生长的影响[J].北方园艺,2012,36(06):172.
REN Yong-quan,YANG Qin,XU Yuan-jiang,et al.Effect of Aqueous Extracts of Pseudostellaria heterophylla on Its Seed Germination and Seedling Growth[J].Northern Horticulture,2012,36(9):172.
[3]任永权,张国辉,周江菊.土壤热处理对连作太子参产量的影响[J].北方园艺,2016,40(07):140.[doi:10.11937/bfyy.201607035]
REN Yongquan,ZHANG Guohui,ZHOU Jiangju.Effect of Soil Heating on Yield of Radix Pseudostellariae in Continuous Cropping[J].Northern Horticulture,2016,40(9):140.[doi:10.11937/bfyy.201607035]
[4]曾维军,曹国璠,李金玲,等.不同坡向对太子参氮含量、生长及产量的影响[J].北方园艺,2018,42(15):159.[doi:10.11937/bfyy.20180537]
ZENG Weijun,CAO Guofan,LI Jinling,et al.Effect of Different Aspects on Nitrogen Content,Growth and Yield of Pseudostellaria heterophylla[J].Northern Horticulture,2018,42(9):159.[doi:10.11937/bfyy.20180537]
[5]余高,陈芬,王海.有机肥替代化肥对土壤养分、酶活性及太子参产质量的影响[J].北方园艺,2023,(14):79.[doi:10.11937/bfyy.20224622]
YU Gao,CHEN Fen,WANG Hai.Effects of Chemical Fertilizer Replacing Organic Fertilizer on Soil Nutrients，Enzyme Activities and Production and Quality of Radix pseudostellariae[J].Northern Horticulture,2023,(9):79.[doi:10.11937/bfyy.20224622]
[6]刘洋,欧小宏,胡艺镨,等.硝态氮对太子参生长与环肽B含量的影响[J].北方园艺,2024,(7):104.[doi:10.11937/bfyy.20233616]
LIU Yang,OU Xiaohong,HU Yipu,et al.Effects of Nitrate Nitrogen on the Growth and Heterophyllin B of Pseudostellaria heterophylla[J].Northern Horticulture,2024,(9):104.[doi:10.11937/bfyy.20233616]
[7]袁青松,高彦平,郭兰萍,等.太子参枯萎病对土壤微生物组成和功能的影响[J].北方园艺,2024,(23):73.[doi:10.11937/bfyy.20241569]
YUAN Qingsong,GAO Yanping,GUO Lanping,et al.Effects of Fusarium Wilt on Soil Microbial Structure and Function of Pseudostellaria heterophylla[J].Northern Horticulture,2024,(9):73.[doi:10.11937/bfyy.20241569]
[8]马志强,曹国璠,姚雨宜,等.配方施肥对太子参品质及产量的影响[J].北方园艺,2025,(11):77.[doi:10.11937/bfyy.20244711]
MA Zhiqiang,CAO Guofan,YAO Yuyi,et al.Effects of Formula Fertilization on Quality and Yield of Pseudostellariae heterophylla[J].Northern Horticulture,2025,(9):77.[doi:10.11937/bfyy.20244711]
备注/Memo
第一作者简介：吕会卿（1998-），男，硕士研究生，研究方向为道地药材生理调控机制。E-mail：1783165336@qq.com.责任作者：欧小宏（1986-），男，博士，讲师，现主要从事中药高效栽培技术等研究工作。E-mail：ogh1986@163.com.基金项目：国家自然科学基金资助项目（82160716）；贵州省基础研究计划（自然科学）资助项目（黔科合基础-ZK［2022］一般464）。收稿日期：2025-09-28