[1]闫恩庆,李铁梅,杨凡,等.树莓果实糖酸积累规律[J].北方园艺,2026,(10):19-28.[doi:10.11937/bfyy.20252587]
　YAN Enqing,LI Tiemei,YANG Fan,et al.Sugar-acid Accumulation Pattern of Raspberry[J].Northern Horticulture,2026,(10):19-28.[doi:10.11937/bfyy.20252587]

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树莓果实糖酸积累规律

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2026年10 页码: 19-28 栏目: 出版日期: 2026-05-30

Title:: Sugar-acid Accumulation Pattern of Raspberry

文章编号:: 1001-0009(2026)10-0019-10

作者:: 闫恩庆1; 2; 3; 李铁梅1; 2; 3; 杨凡1; 2; 3; 凌雪松1; 2; 3; 文莹莹1; 2; 3; 杨国慧1; 2; 3; （1.东北农业大学园艺园林学院,黑龙江哈尔滨 150030；2.东北农业大学农业农村部东北地区园艺作物生物学与种质创制重点实验室，黑龙江哈尔滨 150030；3.东北农业大学寒地小浆果开发利用国家地方联合工程研究中心，黑龙江哈尔滨 150030）

Author(s):: YAN Enqing1; 2; 3; LI Tiemei1; 2; 3; YANG Fan1; 2; 3; LING Xuesong1; 2; 3; WEN Yingying1; 2; 3; YANG Guohui1; 2; 3; (1.School of Horticulture and Landscape，Northeast Agricultural University，Harbin,Heilongjiang 150030；2.Key Laboratory of Biology and Germplasm Innovation of Horticultural Crops in Northeast China，Ministry of Agriculture and Rural Affairs，Northeast Agricultural University，Harbin,Heilongjiang 150030；3.National and Local Joint Engineering Research Center for Development and Utilization of Small Berries in Cold Regions，Northeast Agricultural University，Harbin,Heilongjiang 150030)

关键词:: 树莓; 果实; 糖; 酸; 积累规律

Keywords:: raspberry; fruit; sugar; acid; accumulation pattern

分类号:: S663.2

DOI:: 10.11937/bfyy.20252587

文献标志码:: A

摘要:: 以夏果型树莓‘卡罗琳’和秋果型树莓‘秋福’为试材，测定了树莓果实不同发育时期的糖、酸指标、关键酶活性及基因表达量以及36个品种的成熟果实的糖酸组成，分析了树莓果实糖、酸的积累规律及调控机制，以期为今后采用分子生物技术手段培育优质树莓品种提供参考依据。结果表明：‘卡罗琳’的葡萄糖、蔗糖、山梨醇和总糖含量呈先升后降趋势，果糖持续增加；‘秋福’的葡萄糖、果糖和总糖持续增加，山梨醇和蔗糖先升后降；2个品种的苹果酸、柠檬酸和总酸含量均先升后降；蔗糖磷酸合成酶（SPS）活性、RiSPS基因表达量均在转色期最大，红果期最低；‘卡罗琳’磷酸果糖激酶(PFK)活性、RiPFK基因表达量持续上升，‘秋福’PFK活性表现为升-降-升，其RiPFK基因表达量持续增加；‘卡罗琳’柠檬酸合酶（CS）活性持续上升，‘秋福’CS活性从青果期到白果期下降，之后持续上升。2个品种NAD-MDH活性持续上升，其RiMDH、RiCS基因表达量与酶活性变化相同。36个品种中，还原糖占总糖的77.5%，柠檬酸占总酸的95.9%，表明树莓果实以还原糖和柠檬酸为主要积累成分。

Abstract:: Taking flori-cane raspberry ‘Caroline’ and primo-cane raspberry ‘Autumn Bliss’ as test materials，sugar and acid components，key enzyme activities，and gene expression levels at different developmental stages of the fruits，as well as the sugar and acid composition of mature fruits from 36 varieties were measured,in order to analyze the accumulation patterns and regulatory mechanisms of sugars and acids in raspberry fruits，thereby providing a reference for future molecular breeding strategies aimed at cultivating high-quality raspberry varieties.The results showed that,in ‘Caroline’，glucose，sucrose，sorbitol，and total sugar contents increased initially and then decreased，while fructose exhibited continuous accumulation；in ‘Autumn Bliss’，glucose，fructose，and total sugar demonstrated sustained increases，whereas sorbitol and sucrose displayed an initial rise followed by decline.Both cultivars exhibited similar trends for malic acid content，citric acid content，and total acid content，characterized by an initial increase and decrease subsequently.Activity of SPS and expression of RiSPS gene peaked at the color-turning stage and reached minimal levels at the red fruit stage in both cultivars.Activity of PFK and expression of RiPFK gene showed a continuous increasing in ‘Caroline’.In ‘Autumn Bliss’，activity of PFK exhibited an increase-decrease-increase pattern，while the expression of RiPFK increased continuously.Activity of CS progressively increased in ‘Caroline’，while in ‘Autumn Bliss’，it decreased from the green fruit to white fruit stage then rising.Activity of NAD-MDH increased steadily in both cultivars，corresponding with the expression profiles of RiMDH and RiCS，aligning with enzyme activity dynamics.Among the 36 varieties，reducing sugars accounted for 77.5% of total sugar，while citric acid accounted for 95.9% of total acid，indicating that reducing sugars and citric acid are the predominant accumulating components in raspberry fruits.

参考文献/References:

［1］沈玺龙，李永霞，张群英．树莓的营养及活性成分的研究［J］．安徽农学通报，2019，25(14)：18-19．［2］葛秋来，韩德果，杨国慧，等．不同类型树莓果实生长发育规律研究［J］．湖北农业科学，2012，51(24)：5674-5676．［3］田晓成，祝令成，邹晖，等．果实可溶性糖的积累模式及其调控研究进展［J］．园艺学报，2023，50(4)：885-895．［4］郝瑞鑫，王燕，秦宇，等．不同类型山楂果实发育过程中糖酸积累特性研究［J］．中国果树，2023(8)：30-39．［5］张禹，黄洁帆，乔沈启，等．河北省野生红树莓果实品质差异分析及评价［J］．果树学报，2024，41(4)：712-724．［6］周双，孙兰英，杨光，等.20个品种树莓果实品质评价［J］．食品研究与开发，2022，43(2)：171-176．［7］VILJAKAINEN S，VISTI A,LAAKSO，S.Concentrations of organic acids and soluble sugars in fruits of six Rubus species from Finland［J］Journal of Agricultural and Food Chemistry，2002,50(13):3714-3717.［8］SCHULZ M，CHIM J F．Nutritional and bioactive value of Rubus berries［J］．Food Bioscience，2019（31）：100438．［9］谢娟．红树莓果实生长发育规律及超显微结构研究［D］．哈尔滨：东北农业大学，2011．［10］GRAHAM J，IASI L，MILLAM S．Genotype-specific regeneration from a number of Rubus cultivars［J］．Plant Cell，Tissue and Organ Culture，1997，48(3)：167-173．［11］ERIC V.More attention should be paid on the interpretation of gene expression data［J］.World Journal of Gastroenterology,2012,18(24):3181-3182.［12］VIMOLMANGKANG S，ZHENG H，PENG Q，et al．Assessment of sugar components and genes involved in the regulation of sucrose accumulation in peach fruit［J］．Journal of Agricultural and Food Chemistry，2016，64(35)：6723-6729．［13］REIDEL E J，CHENG L，TURGEON R．(126) morphology and physiology of sugar transport in apple leaves［J］．HortScience，2006，41(4)：1061E-11062．［14］FANG T，CAI Y，YANG Q，et al．Analysis of sorbitol content variation in wild and cultivated apples［J］．Journal of the Science of Food and Agriculture，2020，100(1)：139-144．［15］MIKULIC-PETKOVSEK M，VEBERIC R，HUDINA M，et al．Fruit quality characteristics and biochemical composition of fully ripe blackberries harvested at different times［J］．Foods，2021，10(7)：1581．［16］BASSON C E，GROENEWALD J H，KOSSMANN J，et al．Sugar and acid-related quality attributes and enzyme activities in strawberry fruits：Invertase is the main sucrose hydrolysing enzyme［J］．Food Chemistry，2010，121(4)：1156-1162．［17］杨国慧，辛月岩，麻世琳，等．乙烯处理对树莓果实呼吸速率及乙烯合成代谢的影响［J］．东北农业大学学报，2020，51(1)：33-41，64．［18］于建强，顾凯迪，王传增，等．苹果磷酸果糖激酶基因MdPFPβ调控果实可溶性糖积累的功能［J］．园艺学报，2022，49(10)：2223-2235．［19］YU J Q，GU K D，ZHANG L L，et al.MdbHLH3 modulates apple soluble sugar content by activating phosphofructokinase gene expression［J］.植物学报（英文版），2022，64(4):17.［20］DURN-SORIA S，POTT D M，OSORIO S，et al．Sugar signaling during fruit ripening［J］．Frontiers in Plant Science，2020，11：564917．［21］LI B，ZHU L，YANG N，et al．Transcriptional landscape and dynamics involved in sugar and acid accumulation during apple fruit development［J］．Plant Physiology，2024，195(4)：2772-2786．［22］董书琦，刘海婷，段可，等．不同草莓资源果实有机酸的积累动态及其多样性分析［J］．上海农业学报，2023，39(1)：26-31．［23］陈雷，齐希梁，石彩云，等．园艺作物果实苹果酸代谢与转运及其调控研究进展［J］．果树学报，2023，40(12)：2598-2609．［24］李俊豪，葛晓兰，朱杰，等．桃果实糖酸代谢调控机制研究进展［J］．果树学报，2025，42(6)：1330-1341．［25］ZHANG L，WANG C，JIA R，et al．Malate metabolism mediated by the cytoplasmic malate dehydrogenase gene MdcyMDH affects sucrose synthesis in apple fruit［J］．Horticulture Research，2022（9）：uhac194．［26］YANG M，HOU G，PENG Y，et al．〖STBX〗FaGAPC2/FaPKc2.2〖STBZ〗 and FaPEPCK reveal differential citric acid metabolism regulation in late development of strawberry fruit［J］．Frontiers in Plant Science，2023（14）：1138865．

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备注/Memo

第一作者简介：闫恩庆（1997-），男，硕士研究生，研究方向为果树种质资源。E-mail：yeq82338765@163.com.责任作者：杨国慧(1969-)，女，博士，教授，硕士生导师，现主要从事小浆果种质资源与栽培生理等研究工作。E-mail：guohuiyang@neau.edu.cn.基金项目：寒地特色果树产业关键技术研究与应用示范资助项目（2022YFD1600500）。收稿日期：2025-07-16

更新日期/Last Update: 2026-06-04