ZHANG Xiaoshen,ZHANG Zhenhua,CAO Hui,et al.Identification and Expression Pattern Analysis of Transcription Factor TCP Family in Leaves and Flowers of Lonicera japonica[J].Northern Horticulture,2025,(22):19-26.[doi:10.11937/bfyy.20251556]
转录因子TCP家族在金银花叶片和花中的鉴定及表达模式分析
- Title:
- Identification and Expression Pattern Analysis of Transcription Factor TCP Family in Leaves and Flowers of Lonicera japonica
- 文章编号:
- 1001-0009(2025)22-0019-08
- Keywords:
- Lonicera japonica; transcription factor TCP; bioinformatics; differential expression pattern
- 分类号:
- S 567.79
- 文献标志码:
- A
- 摘要:
- 以金银花‘郑银花3号’的花和叶片为试材，构建了2个金银花转录组测序文库，采用生物信息学方法，研究了金银花TCP转录因子的蛋白理化性质、二级结构、保守基序及系统进化树进行分析，进一步对TCP家族在金银花叶片和花的差异表达模式进行分析，以期研究出金银花花和叶中TCP转录因子差异表达。结果表明：金银花TCP家族20个成员中有呈酸性的蛋白7个和呈碱性的蛋白13个；20个TCP蛋白均呈现亲水性；TCP蛋白二级结构的占比呈现为无规则卷曲＞α-螺旋＞延伸链＞β-转角，但Lj8E152T0的TCP蛋白的α-螺旋占比小于延伸链占比。根据基序结果分析发现，出现频率最高的是Motif 1、Motif 2、Motif 4和Motif 5。基因热图结果显示Lj7C10T1在花中的表达量显著高于在叶片中的表达量，其次表达差异较大的就是Lj4C131T10和LjC716T2，同时这3个基因也是在金银花的花中表达量最高的基因。
参考文献/References:
［1］高琪，张德斌，高邵晨，等．叶面喷施亚硒酸钠对金银花有效成分含量及品质的影响［J］．北方园艺，2024(18)：92-99．［2］何海燕，孟盼盼，于白音，等．忍冬入药部位考证及现代研究概况［J］．韶关学院学报，2023，44(3)：68-72．［3］章艳玲．金银花不同部位化学成分的比较研究［J］．农学学报，2019，9(1)：59-61．［4］李文略，柳婷婷，陈常理，等．植物TCP蛋白作用机制研究进展［J］．分子植物育种，2023，21(14)：4650-4658．［5］CUBAS P，LAUTER N，DOEBLEY J，et al．The TCP domain：A motif found in proteins regulating plant growth and development［J］．The Plant Journal，1999，18(2)：215-222．［6］DOEBLEY J，STEC A，HUBBARD L．The evolution of apical dominance in maize［J］．Nature，1997，386(6624)：485-488．［7］LUO D，CARPENTER R，VINCENT C，et al．Origin of floral asymmetry in Antirrhinum［J］．Nature，1996，383(6603)：794-799．［8］KOSUGI S，OHASHI Y．PCF1 and PCF2 specifically bind to Cis elements in the rice proliferating cell nuclear antigen gene［J］．The Plant Cell，1997，9(9)：1607-1619．［9］谭政委，郭水柱，苏小雨，等．全基因组水平金银花TCP基因家族的鉴定及表达模式分析［J］．中草药，2024，55(5)：1665-1676．［10］RESENTINI F，FELIPO-BENAVENT A，COLOMBO L，et al．TCP14 and TCP15 mediate the promotion of seed germination by gibberellins in Arabidopsis thaliana［J］．Molecular Plant，2021，14(10)：1771．［11］张雪莹.番茄叶形发育关键TCP转录因子筛选及SlTCP24功能解析［D］.沈阳：沈阳农业大学,2022.［12］刘俊，李龙，吴耀松，等．杜仲TCP转录因子鉴定及生物信息学分析［J］．中草药，2022，53(12)：3755-3765．［13］杜洋，张智韦，陆爽，等．菊苣TCP基因家族鉴定与分析［J］．草地学报，2024，32(8)：2428-2439．［14］曾家晶，赵怡娇，曾东琳，等．芥蓝TCP家族全基因组鉴定及表达分析［J］．中国农业大学学报，2022，27(8)：76-92．［15］DOEBLEY J，STEC A，GUSTUS C．Teosinte branched1 and the origin of maize：Evidence for epistasis and the evolution of dominance［J］．Genetics，1995，141(1)：333-346．［16］李晓静，李圣龙，岳亮亮．石榴TCP基因家族的生物信息学与表达分析［J］．江苏农业科学，2024，52(1)：41-48．［17］杜姗姗，周绍礼，赵宝林，等．豌豆TCP基因家族的鉴定与分析［J］．植物生理学报，2022，58(8)：1575-1586．［18］齐香玉，李新茹，陈双双，等．茉莉花TCP基因家族全基因组鉴定及其表达分析［J］．华北农学报，2024，39(1)：63-71．［19］吕婷婷．吉林人参TCP转录因子家族的系统分析及PgTCP26-02基因功能研究［D］．长春：吉林农业大学，2024．［20］AGUILAR-MARTNEZ J A，SINHA N.Analysis of the role of Arabidopsis class I TCP genes AtTCP7，AtTCP8，AtTCP22，and AtTCP23 in leaf development［J］.Front Plant Sci,2013,16(4):406.［21］LI S，ZACHGO S．TCP3 interacts with R2R3-MYB proteins，promotes flavonoid biosynthesis and negatively regulates the auxin response in Arabidopsis thaliana［J］．The Plant Journal，2013，76(6)：901-913．［22］赵媛媛，杨倩茹，郝江波，等．金银花与忍冬藤及叶药理作用差异的研究进展［J］．中国中药杂志，2016，41(13)：2422-2427．［23］CUBAS P，COEN E，ZAPATER J M M．Ancient asymmetries in the evolution of flowers［J］．Current Biology，2001，11(13)：1050-1052．［24］BUSCH A，ZACHGO S．Control of Corolla monosymmetry in the Brassicaceae Iberis amara［J］．Proceedings of the National Academy of Sciences of the United States of America，2007，104(42)：16714-16719．［25］KIEFFER M，MASTER V，WAITES R，et al.TCP14 and TCP15 affect internode length and leaf shape in Arabidopsis［J］.The Plant Journal,2011,68(1):147-158.［26］STEINER E，EFRONI I，GOPALRAJ M，et al．The Arabidopsis O-linked N-acetylglucosamine transferase SPINDLY interacts with class I TCPs to facilitate cytokinin responses in leaves and flowers［J］．The Plant Cell，2012，24(1)：96-108．［27］KOYAMA T，FURUTANI M，TASAKA M，et al．TCP transcription factors control the morphology of shoot lateral organs via negative regulation of the expression of boundary-specific genes in Arabidopsis［J］．The Plant Cell，2007，19(2)：473-484．［28］WEI B，ZHANG J，PANG C，et al．The molecular mechanism of sporocyteless/nozzle in controlling Arabidopsis ovule development［J］．Cell Research，2015，25(1)：121-134．［29］FINLAYSON S A．Arabidopsis Teosinte Branched1-like 1 regulates axillary bud outgrowth and is homologous to monocot Teosinte Branched1［J］．Plant & Cell Physiology，2007，48(5)：667-677．［30］AGUILAR-MARTNEZ J A，POZA-CARRIN C，CUBAS P．Arabidopsis BRANCHED1 acts as an integrator of branching signals within axillary buds［J］．The Plant Cell，2007，19(2)：458-472．［31］HE Z，ZHAO X，KONG F，et al．TCP2 positively regulates HY5/HYH and photomorphogenesis in Arabidopsis［J］．Journal of Experimental Botany，2016，67(3)：775-785．［32］HERV C，DABOS P，BARDET C，et al．In vivo interference with AtTCP20 function induces severe plant growth alterations and deregulates the expression of many genes important for development［J］．Plant Physiology，2009，149(3)：1462-1477．［33］PRUNEDA-PAZ J L，BRETON G，PARA A，et al．A functional genomics approach reveals CHE as a component of the Arabidopsis circadian clock［J］．Science，2009，323(5920)：1481-1485．［34］VIOLA I L，UBERTI MANASSERO N G，RIPOLL R，et al．The Arabidopsis class I TCP transcription factor AtTCP11 is a developmental regulator with distinct DNA-binding properties due to the presence of a threonine residue at position 15 of the TCP domain［J］．Biochemical Journal，2011，435(1)：143-155．
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备注/Memo
第一作者简介：张晓申（1976-），男，博士，副研究员，现主要从事中药材新品种选育及栽培等研究工作。E-mail：nkszyc@126.com.责任作者：牛苏燕（1986-），女，博士，副教授，现主要从事中药材种质资源评价及栽培等研究工作。E-mail：niusuyan1234@163.com.基金项目：河南省科技兴林豫财环资资助项目(［2023］60号,［2025］44号)。收稿日期：2025-04-25