XU Yan,LI Yudie,CHEN Siqi,et al.Pathogen Identification and In Vitro Inhibiton of Root Rot Disease From Hippeastrum rutilum[J].Northern Horticulture,2025,(18):95-101.[doi:10.11937/bfyy.20250616]
朱顶红根腐病病原菌的鉴定及体外抑制作用研究
- Title:
- Pathogen Identification and In Vitro Inhibiton of Root Rot Disease From Hippeastrum rutilum
- 文章编号:
- 1001-0009(2025)18-0095-07
- 分类号:
- S 436.8
- 文献标志码:
- A
- 摘要:
- 以不同品种的朱顶红(Hippeastrum rutilum)发病组织为试材，采用组织分离法对病原菌进行分离，并对分离出的病原菌进行了形态学观察和ITS序列分析鉴定，并探究了3种不同浓度(0.01、0.02、0.04 g·mL-1)的5种农药(百菌清、多菌灵、代森锰锌、甲基硫菌灵、菌核净)对分离出的病原菌的体外抑制作用，以期为朱顶红根腐病病原菌的分离鉴定及防治提供参考依据。结果表明：从朱顶红发病样品中共分离出了25个单菌株，根据菌落性状分为3类菌，ITS序列分析发现25个单菌株实际上只有3种基因序列，通过BLAST初步鉴定均属于镰刀菌属(Fusarium)。此外，5种农药对3种朱顶红根腐病病原菌的体外抑制作用不同，其中有7个处理组具有不同程度的显著性抑制作用。
- Abstract:
- Taking different varieties of Hippeasterum rutilum diseased tissues as the test materials,the pathogenic bacteria were isolated by tissue isolation method,and the isolated pathogenic bacteria were observed morphologically and identified by ITS sequence analysis.The in vitro inhibitory effects of five pesticides (hlorothalonil,carbendazim,mancozed,thiophanate-methyl,and dimetachlone) at three different concentrations (0.01,0.02,0.04 g·mL-1) on the isolated pathogenic bacteria were explored,in order to provide reference for the isolation,identification,and prevention of root rot pathogens in Hippeasterum rutilum.The results showed that a total of 25 single bacterial strains were isolated from the samples of the Hippeastrum rutilum diseased plant，which were classified into three types of bacteria based on their colony characteristics.ITS sequence analysis revealed that the 25 single bacterial strains actually only had three gene sequences，and were preliminarily identified by BLAST as belonging to the genus Fusarium.In addition，the in vitro inhibitory effects of five pesticides on three types strains of root rot disease from Hippeastrum rutilum were different，with seven treatments showing significant inhibitory effects to varying degrees.
参考文献/References:
［1］于波，孙映波，刘小飞，等.香味朱顶红新品种豆蔻的选育［J］.热带农业科学，2024，44(8)：42-47.［2］金晨莺,张海珍,沈笑,等.朱顶红促成栽培技术研究进展与园林应用［J］.浙江农业科学,2020,61(1):72-75.［3］杨柳燕,李青竹,蔡友铭,等.二十四个朱顶红品种观赏性状分析及杂交育种研究［J］.北方园艺,2019(1):109-114.［4］蔡梦真，张宇，刘海涛.朱顶红新品种‘华丹’［J］.园艺学报，2023，50(S2)：131-132.［5］黄宝华,蔡家珍,张梓浩,等.朱顶红转录组De novo数据及功能注释分析［J］.分子植物育种,2021,19(2):432-440.［6］杨维，刘心怡，曾晶珏，等.朱顶红曼谷玫瑰愈伤组织途径再生体系的建立［J］.热带作物学报，2023，44(5)：977-985.［7］吕文涛，娄文睿，娄晓鸣，等.基于EST-SSR标记的朱顶红种质资源鉴定［J］.分子植物育种，2024，22(4)：1123-1132.［8］XIONG M,WANG Y,CHEN D,et al.Assessment of genetic diversity and identification of core germplasm in single-flowered Amaryllis (Hippeastrum hybridum) using SRAP markers［J］.Biotechnology & Biotechnological Equipment,2020,34(1):966-974.［9］JAMIL M K,RAHMAN M M,HOSSAIN M M,et al.Influence of sucrose and aluminium sulphate vase life of cut Hippeastrum flower (Hippeastrum hybridum Hort.) as influenced［J］.Bangladesh Journal of Agricultural Research,2016,41(2):221-234.［10］WANG S,LIU X,LIU X,et al.The red/blue light ratios from light-emitting diodes affect growth and flower quality of Hippeastrum hybridum ‘Red Lion’［J］.Frontiers in Plant Science,2022,13:1048770.［11］VAZQUEZ C,REED S T,DUNN C.Nitrogen fertilization as ammonium or nitrate-N on Hippeastrum hybridum bulb growth［J］.Agricultural Sciences,2015,6(12):1547-1554.［12］WANG Y,CHEN D,HE X,et al.Revealing the complex genetic structure of cultivated Amaryllis (Hippeastrum hybridum) using transcriptome-derived microsatellite markers［J］.Scientific Reports,2018,8:10645.［13］孟庆忠,李孝鉴,易先达,等.朱顶红根腐病病原菌的鉴定［J］.湖北农业科学,2020,59(24):99-100,116.［14］鲁宪.朱顶红根腐病的防治［J］.中国花卉盆景,1992(2):12.［15］吕英民，王有江.朱顶红［M］.北京：中国林业大学出版社，2014.［16］李云芳.菌核净在土壤、番茄和水稻中的残留行为及其代谢物筛查［D］.贵阳:贵州大学,2023.［17］杨永青,高婧,杨志刚,等.番茄疫霉根腐病病原菌鉴定及药剂敏感性分析［J］.山西农业大学学报(自然科学版),2024,44(5):11-19.［18］陈少萍.朱顶红栽培与病虫害防治［J］.中国花卉园艺,2014(16):32-34.［19］吴琪.大连市草坪病害调查及草坪草黑孢霉叶斑病的初步研究［D］.长春:吉林农业大学,2008.［20］龚国华.高效液相色谱法同时检测百菌清和环丙唑醇［J］.浙江农业科学，2024，65(8)：1923-1928.［21］何长春，郝梓瑶，张海立，等.几种抑菌剂对大白杜鹃组培苗内生菌的抑菌效果［J/OL］.分子植物育种,（2025-07-01）［2025-08-01］.https://link.cnki.net/urlid/46.1068.S.20241206.1615.018.［22］陈晨，廖佳兴，王琦.葡萄炭疽病菌防治技术研究［J］.中国南方果树，2023，52(5)：157-161.［23］施心成,李涛,张传清.山核桃干腐病菌对甲基硫菌灵等4种杀菌剂的抗性［J］.农药学学报,2023,25(6):1288-1294.
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备注/Memo
第一作者简介：徐艳（1981-），女，博士，副教授，现主要从事病原与寄主互作等研究工作。E-mail:xuyan2018@jcut.edu.cn.责任作者：易庆平（1981-），男，博士，教授，现主要从事特色花卉等研究工作。E-mail:yiqingping@jcut.edu.cn.基金项目：湖北省荆门市引导性科学研究计划资助项目（2023YDKY064）；湖北省高等学校优秀中青年科技创新团队计划资助项目（T2023032）；湖北省中央引导地方科技发展专项资助项目（2022BGE262）；荆楚理工学院大学生创新创业资助项目（S202411336026）。收稿日期：2025-02-24