LIU Caixia,SUO Xiaoxiong,SUN Nini,et al.Transcriptomic Analysis of Ziziphus jujuba of Two Different Fruit Shapes[J].Northern Horticulture,2025,(18):1-10.[doi:10.11937/bfyy.20250449]
两种不同果形酸枣的转录组比较分析
- Title:
- Transcriptomic Analysis of Ziziphus jujuba of Two Different Fruit Shapes
- 文章编号:
- 1001-0009(2025)18-0001-10
- Keywords:
- Ziziphus jujuba; fruit shape; transcriptome; mechanism; genes
- 分类号:
- S 662.9
- 文献标志码:
- A
- 摘要:
- 以圆形和椭圆形酸枣果肉及种子为试材，采用高通量转录组测序方法，研究了不同果形在基因表达水平的差异，并推测参与黄酮类生物合成的关键基因，以期为黄酮类化合物的生物合成及酸枣的品种选育提供参考依据。结果表明：2种不同果形酸枣果肉之间共存在2 349个差异表达基因（differentially expressed genes），种子之间共存在407个DEGs，在KEGG富集分析中发现，2种不同果形酸枣果肉中有75个参与黄酮类生物合成的DEGs；种子中仅有12个参与其合成的DEGs，基因FLS、F3H、CHS、HCT、CYP73A在椭圆形酸枣果肉中的表达量高于圆形酸枣果肉。
- Abstract:
- Taking pulp and seeds from round and oval Z.jujube fruits as the test materials，high-throughput transcriptome sequencing was used to compare pulp and seeds from round and oval Z.jujube fruits.Differentially expressed genes (DEGs) were analyzed，and key genes involved in flavonoid biosynthesis were identified,in order to provide references for analyses of the biosynthesis of flavonoids and breeding of Z.jujube.The results showed that 2 349 DEGs between the two fruit shapes in the pulp and 407 DEGs in the seeds.In a Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis 75 DEGs were involved in flavonoid biosynthesis in the pulp samples and 12 DEGs were involved in this pathway in the seeds.Expression levels of the candidate genes FLS，F3H，CHS，HCT，and CYP73A were higher in the pulp of oval Z.jujube than in that of round Z.jujube.
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备注/Memo
第一作者简介：刘彩霞（1999-），女，硕士研究生，研究方向为中药资源开发与品质评价。E-mail:1847053381@qq.com.责任作者：裴香萍（1969-），女，硕士，教授，现主要从事中药资源与利用等研究工作。E-mail:peixp69@163.com.基金项目：国家自然科学基金青年科学基金资助项目（81603251）；山西省科技厅基础研究资助项目（202403021221198）；山西省科学技术厅科技创新人才重点团队资助项目（202304051001020）；山西省研究生实践创新资助项目（2024SJ339）。收稿日期：2025-02-17