WANG Yun,ZHANG Changqing,TIAN Wen,et al.Research Progress on Antimicrobial Peptides for the Prevention and Control of Plant Diseases[J].Northern Horticulture,2025,(12):126-133.[doi:10.11937/bfyy.20250003]
抗菌肽在防治植物病害中的研究进展
- Title:
- Research Progress on Antimicrobial Peptides for the Prevention and Control of Plant Diseases
- 文章编号:
- 1001-0009(2025)12-0126-08
- 分类号:
- S 471
- 文献标志码:
- A
- 摘要:
- 抗菌肽是新一代对抗耐药性病原体的候选抗菌剂，在医药、食品和农业领域得到广泛应用。该研究综述了抗菌肽的优化与合成以及数据库的计算、挖掘和虚拟筛选方法，重点关注了国内外利用抗菌肽防治植物病害的研究现状和作用机制,以期为抗菌肽在种植领域的应用提供新的有价值的参考依据。
- Abstract:
- Antimicrobial peptides (AMPs) are a new generation of candidate antimicrobial agents against drug-resistant pathogens，and have been widely used in medicine，food and agriculture.In this study，the optimization and synthesis of antimicrobial peptides as well as the methods of database calculation，mining and virtual screening were reviewed，focusing on the research status and mechanism of the use of antimicrobial peptides to control plant diseases，in order to provide a new reference for the application of antimicrobial peptides in the field of planting.
参考文献/References:
［1］刘芹,杨俊杰,杨昱丹,等.2024年湖北省农作物主要病虫害发生趋势［J］.湖北植保,2024(2):65-68.［2］吴思炫,高复云,张锐澎,等.番茄青枯病生物防治的研究进展［J］.应用生态学报,2023,34(9):2585-2592.［3］黄光耀,蒙麒元,林林,等.几种药剂对柑橘溃疡病的田间防治效果［J］.广西植保,2024,37(1):7-9.［4］宋春华,马骞,苏佳萌,等.宁南霉素对水稻纹枯病的防治试验［J］.农药,2024,63(3):217-223.［5］谭海军.中国生物农药的概述与展望［J］.世界农药,2022,44(4):16-27,54.［6］潘群胜.木霉菌在植物病害生物防治中的作用［J］.现代化农业,2023(6):8-10.［7］杨悦,李燕,王小方,等.抗菌肽及其在食物储藏与保鲜中的应用［J］.食品与生物技术学报,2021,40(4):9-16.［8］杨晨远,于子川,秦迪,等.抗菌肽的结构分析、抗菌机制及改造应用的研究进展［J］.微生物学报,2024,64(7):2242-2259.［9］MAKOVITZKI A,AVRAHAMI D,SHAI Y.Ultrashort antibacterial and antifungal lipopeptides［J］.Proceedings of the National Academy of Sciences of the United States of America,2006,103(43):15997-16002.［10］段世佳.食用抗菌肽制备的中试优化及其在食品保鲜中的应用［D］.石家庄:河北科技大学,2012.［11］谢咸升,董建臻,李静,等.抗菌肽控制植物病害研究进展及展望［J］.河北农业科学,2011,15(2):46-49.［12］贾艳丽,韩紫薇,仇燕.新型抗菌肽对番茄灰霉病的防治效果与机理的研究［J］.北方园艺,2023(18):18-27.［13］彭洪海,罗玉萍,李思光.抗菌肽及抗菌肽转基因植物研究进展［J］.生物技术通报,2007,23(1):28-32.［14］冉瑜.新型抗菌肽与磷脂膜的相互作用研究［D］.郑州:郑州大学,2013.［15］NAKATSUJI T,GALLO R L.Antimicrobial peptides:Old molecules with new ideas［J］.Journal of Investigative Dermatology,2012,132(3):887-895.［16］ZHANG Q Y，YAN Z B，MENG Y M，et al.Antimicrobial peptides：Mechanism of action，activity and clinical potential［J］.中国人民解放军军医大学学报（英文版），2022(2)：231-258.［17］YONEYAMA F,IMURA Y,OHNO K,et al.Peptide-lipid huge toroidal pore,a new antimicrobial mechanism mediated by a lactococcal bacteriocin,lacticin Q［J］.Antimicrobial Agents and Chemotherapy,2009,53(8):3211-3217.［18］WEI S,BROOKS C L.Stability and orientation of cecropin P1 on maleimide self-assembled monolayer (SAM) surfaces and suggested functional mutations［J］.Chinese Chemical Letters,2015,26(4):485-490.［19］ZHANG S,LUO L,SUN X,et al.Bioactive peptides:A promising alternative to chemical preservatives for food preservation［J］.Journal of Agricultural and Food Chemistry,2021,69(42):12369-12384.［20］ZAI Y,XI X P,YE Z M,et al.Aggregation and its influence on the bioactivities of a novel antimicrobial peptide,temporin-PF,and its analogues［J］.International Journal of Molecular Sciences,2021,22(9):4509.［21］FABBRETTI A,BRANDI L,PETRELLI D,et al.The antibiotic Furvina targets the P-site of 30S ribosomal subunits and inhibits translation initiation displaying start Codon bias［J］.Nucleic Acids Research,2012,40(20):10366-10374.［22］张丽慧,雷茹林,胡美忠.抗菌肽的来源、活性及作用机制最新研究进展［J］.食品安全质量检测学报,2023,14(9):1-8.［23］KUMAR R,ALI S A,SINGH S K,et al.Antimicrobial peptides in farm animals:An updated review on its diversity,function,modes of action and therapeutic prospects［J］.Veterinary Sciences,2020,7(4):206.［24］CHO J,HWANG I S,CHOI H,et al.The novel biological action of antimicrobial peptides via apoptosis induction［J］.Journal of Microbiology and Biotechnology,2012,22(11):1457-1466.［25］FU J,ZONG X,JIN M L,et al.Mechanisms and regulation of defensins in host defense［J］.Signal Transduction and Targeted Therapy,2023,8(1):300.［26］DAS K,DATTA K,KARMAKAR S,et al.Antimicrobial peptides-small but mighty weapons for plants to fight phytopathogens［J］.Protein and Peptide Letters,2019,26(10):720-742.［27］SANTOS-JUNIOR C D,TORRES M D T,DUAN Y Q,et al.Discovery of antimicrobial peptides in the global microbiome with machine learning［J］.Cell,2024,187(14):3761-3778.e16.［28］田源，韩爱萍，徐春明.深度学习与分子模拟相结合:高效筛选宏基因组数据中的抗菌肽［J/OL］.食品科学技术学报，(2024-03-13)［2024-12-12］.http://kns.cnki.net/kcms/detail/10.1151.TS.20240312.1743.003.html.［29］薛凤,封硕,李菁.人工智能方法在抗菌肽筛选领域的应用及展望［J］.中国药科大学学报,2023,54(3):314-322.［30］苏琰,李融.抗菌肽的食品保鲜应用及生物合成研究进展［J］.食品与机械,2024,40(7):208-215.［31］JAISWAL M,SINGH A,KUMAR S.PTPAMP:Prediction tool for plant-derived antimicrobial peptides［J］.Amino Acids,2023,55(1):1-17.［32］YANG B,YANG H Y,LIANG J L,et al.A review on the screening methods for the discovery of natural antimicrobial peptides［J］.Journal of Pharmaceutical Analysis,2025,15(1):101046.［33］LEE E Y,FULAN B M,WONG G C L,et al.Mapping membrane activity in undiscovered peptide sequence space using machine learning［J］.Proceedings of the National Academy of Sciences of the United States of America,2016,113(48):13588-13593.［34］DAS P,SERCU T,WADHAWAN K,et al.Accelerated antimicrobial discovery via deep generative models and molecular dynamics simulations［J］.Nature Biomedical Engineering,2021,5(6):613-623.［35］汪庆,张瑞芬,王亚楠,等.抗菌肽结构改造与人工智能研发策略［J］.微生物学报,2022,62(11):4353-4366.［36］周欣宇,周春才.抗菌肽及类抗菌肽的设计、合成及应用［J］.化学进展,2018,30(7):913-920.［37］魏岱旭,龚海伦,张旭维.抗菌肽的生物合成及医学应用［J］.合成生物学,2022,3(4):709-727.［38］邵长轩,付艳雪,方禹鑫,等.基因工程法在微生物系统表达抗菌肽的研究进展［J］.微生物学报,2024,64(10):3620-3632.［39］GONG G L,WEI Y,WANG Z Z.Functional expression,purification,and antimicrobial activity of a novel antimicrobial peptide MLH in Escherichia coli［J］.Preparative Biochemistry & Biotechnology,2018,48(1):57-63.［40］JING X L,LUO X G,TIAN W J,et al.High-level expression of the antimicrobial peptide plectasin in Escherichia coli［J］.Current Microbiology,2010,61(3):197-202.［41］LEE J H,MINN I,PARK C B,et al.Acidic peptide-mediated expression of the antimicrobial peptide buforin Ⅱ as tandem repeats in Escherichia coli［J］.Protein Expression and Purification,1998,12(1):53-60.［42］WANG Q,ZHU F F,XIN Y Q,et al.Expression and purification of antimicrobial peptide buforin Ⅱb in Escherichia coli［J］.Biotechnology Letters,2011,33(11):2121-2126.［43］ZHANG Y,WANG Y P,LU J G,et al.High-yield and cost-effective biosynthesis process for producing antimicrobial peptide AA139［J］.Protein Expression and Purification,2024,219:106475.［44］MONTFORT-GARDEAZABAL J M,BALDERAS-RENTERIA I,CASILLAS-VEGA N G,et al.Expression and purification of the antimicrobial peptide Bin1b in Escherichia coli tagged with the fusion proteins CusF3H+ and SmbP［J］.Protein Expression and Purification,2021,178:105784.［45］刘扬科,赵天效,卢晓颖,等.枯草芽孢杆菌YT168-6产抗菌肽sublancin的发酵工艺优化及培养基筛选的研究［J］.中国畜牧兽医,2021,48(9):3232-3241.［46］LUAN C,ZHANG H W,SONG D G,et al.Expressing antimicrobial peptide cathelicidin-BF in Bacillus subtilis using SUMO technology［J］.Applied Microbiology and Biotechnology,2014,98(8):3651-3658.［47］ZHANG L C,LI G Q,ZHAN N,et al.Expression of a Pseudomonas aeruginosa-targeted antimicrobial peptide T9W in Bacillus subtilis using a maltose-inducible vector［J］.Process Biochemistry,2019,81:22-27.［48］LEE B C,TSAI J C,LIN C Y,et al.Using Bacillus subtilis as a host cell to express an antimicrobial peptide from the marine chordate Ciona intestinalis［J］.Marine Drugs,2021,19(2):111.［49］孔熹.利用枯草芽孢杆菌表达系统进行大蒜抗菌基因筛选［D］.武汉:华中农业大学,2019.［50］ZHANG L C,WEI D D,ZHAN N,et al.Heterologous expression of the novel α-helical hybrid peptide PR-FO in Bacillus subtilis［J］.Bioprocess and Biosystems Engineering,2020,43(9):1619-1627.［51］查曼，闵勇，刘晓艳，等.抗菌肽在毕赤酵母中表达的研究进展［J/OL］.食品与发酵工业,(2024-11-29)［2025-01-01］,https://doi.org/10.13995/j.cnki.11-1802/ts.041234.［52］LI M R,ZHOU R N,WANG Y Y,et al.Heterologous expression of frog antimicrobial peptide Odorrana in-C1 in Pichia pastoris:Biological characteristics and its application in food preservation［J］.Journal of Biotechnology,2024,390:50-61.［53］MENG D M,ZHAO J F,LING X,et al.Recombinant expression,purification and antimicrobial activity of a novel antimicrobial peptide PaDef in Pichia pastoris［J］.Protein Expression and Purification,2017,130:90-99.［54］MORIDI K,HEMMATY M,AKBARI EIDGAHI M R,et al.Construction,cloning,and expression of Melittin antimicrobial peptide using Pichia pastoris expression system［J］.Gene Reports,2020,21:100900.［55］XING L W,TIAN S X,GAO W,et al.Recombinant expression and biological characterization of the antimicrobial peptide fowlicidin-2 in Pichia pastoris［J］.Experimental and Therapeutic Medicine,2016,12(4):2324-2330.［56］宗西翠,张翼,方彭华,等.抗菌肽重组表达的优化策略［J］.中国生物制品学杂志,2022,35(4):493-499,507.［57］韩素冰.两种抗菌肽CATHPb1和As-CATH4在亚心型四爿藻中的串联表达及功能分析［D］.烟台:烟台大学,2024.［58］DONG B,CHENG R Q,LIU Q Y,et al.Multimer of the antimicrobial peptide mytichitin-a expressed in Chlamydomonas reinhardtii exerts a broader antibacterial spectrum and increased potency［J］.Journal of Bioscience and Bioengineering,2018,125(2):175-179.［59］NAZARIAN-FIROUZABADI F,DER TOROSSIAN TORRES M,de LA FUENTE-NUNEZ C.Recombinant production of antimicrobial peptides in plants［J］.Biotechnology Advances,2024,71:108296.［60］HOLASKOVA E,GALUSZKA P,FREBORT I,et al.Antimicrobial peptide production and plant-based expression systems for medical and agricultural biotechnology［J］.Biotechnology Advances,2015,33(6):1005-1023.［61］GHIDEY M,ASHIQUL ISLAM S M,PRUETT G,et al.Making plants into cost-effective bioreactors for highly active antimicrobial peptides［J］.New Biotechnology,2020,56:63-70.［62］IRALU N,WANI S,MEHRAJ I,et al.Antimicrobial peptides from plants and microorganisms for plant disease management［J］.Plant Pathology,2024,73(7):1691-1707.［63］PAUL M,CHOWDHURY T,SAHA S.Antimicrobial peptide:A competent tool for plant disease control in mulberry-a review［J］.Vegetos,2023,36(3):733-742.［64］石伟.蜂毒肽melittin对水稻白叶枯病菌作用机制及抗菌肽溶血性研究［D］.南京:南京师范大学,2015.［65］邢卓.蜂毒肽毒杀作物病原菌机理和植生效应［D］.大连:辽宁师范大学,2005.［66］曹鹏,周金环,王新亮,等.发根农杆菌介导的柑橘黄龙病抗性快速评价体系优化及应用［J］.中国农业科学,2024,57(16):3182-3191.［67］何佳玲.植物源抗菌肽MaSAMP的活性分析及高效表达［D］.武汉:湖北大学,2024.［68］沈辉.刺肩蝽Thanatin抗菌肽基因对烟草青枯病耐受性影响［D］.重庆:西南大学,2018.［69］李广,李晓芬,易兰花.拮抗菌枯草芽孢杆菌1151及其所产抗菌肽对辣椒采后软腐病的控制作用［J］.食品与发酵工业,2023,49(10):78-84.［70］贾士荣,屈贤铭,冯兰香,等.转抗菌肽基因提高马铃薯对青枯病的抗性［J］.中国农业科学,1998,31(3):5-12.［71］NING W M,LUO X W,ZHANG Y,et al.Broad-spectrum nano-bactericide utilizing antimicrobial peptides and bimetallic Cu-Ag nanoparticles anchored onto multiwalled carbon nanotubes for sustained protection against persistent bacterial pathogens in crops［J］.International Journal of Biological Macromolecules,2024,265:131042.［72］MOHAMED G,JI A,CAO X Y,et al.A small antimicrobial peptide derived from a Burkholderia bacterium exhibits a broad-spectrum and high inhibiting activities against crop diseases［J］.Plant Biotechnology Journal,2025,23(2):430-441.［73］PANTHI U,MCCALLUM B C,KOVALCHUK I,et al.Foliar application of plant-derived peptides decreases the severity of leaf rust (Puccinia triticina) infection in bread wheat (Triticum aestivum L.)［J］.Journal of Genetic Engineering and Biotechnology,2024,22(1):100357.［74］王财成.贝莱斯芽孢杆菌抗菌肽对百香果致病性腐皮镰刀菌的生防潜力研究［D］.福州:福建农林大学,2020.［75］刘莎.抗菌肽mastoparan-S，thanatin和ponericin W1对柑橘果实酸腐病的控制效果与机制研究［D］.重庆：西南大学,2019.［76］NAZARI Z,NAZARIAN-FIROUZABADI F,ISMAILI A,et al.Transgenic tobacco plants expressing a chimeric antimicrobial protein show disease resistance to plant diseases［J］.Physiological and Molecular Plant Pathology,2023,127:102083.［77］YANG X M,WANG Y T,JIANG H Y,et al.Antimicrobial peptide CB-M exhibits direct antifungal activity against Botrytis cinerea and induces disease resistance to gray mold in cherry tomato fruit［J］.Postharvest Biology and Technology,2023,196:112184.［78］FAN L,WEI Y Y,CHEN Y,et al.Epinecidin-1,a marine antifungal peptide,inhibits Botrytis cinerea and delays gray mold in postharvest peaches［J］.Food Chemistry,2023,403:134419.［79］FAN L,WEI Y Y,CHEN Y,et al.Transcriptome analysis reveals the mechanism of antifungal peptide epinecidin-1 against Botrytis cinerea by mitochondrial dysfunction and oxidative stress［J］.Pesticide Biochemistry and Physiology,2024,202:105932.［80］郭庆港,刘高鸽,陈秀叶,等.枯草芽胞杆菌HMB19198菌株抑菌物质的鉴定及其对番茄灰霉病的防治［J］.植物病理学报,2022,52(2):247-255.［81］宋雪莹,高圣玥,李平,等.抗菌肽的来源与作用机理及应用［J］.动物医学进展,2024,45(4):112-115.［82］SHABANI S,HADJIGOL S,LI W Y,et al.Synthetic peptide branched polymers for antibacterial and biomedical applications［J］.Nature Reviews Bioengineering,2024,2(4):343-361.［83］LIU H,YU M,ZHOU S D,et al.Unveiling novel anti-viral mechanisms of ε-poly-l-lysine on tobacco mosaic virus-infected Nicotiana tabacum through microRNA and transcriptome sequencing［J］.International Journal of Biological Macromolecules,2024,268:131628.［84］LIU S,WANG W J,DENG L L,et al.Control of sour rot in Citrus fruit by three insect antimicrobial peptides［J］.Postharvest Biology and Technology,2019,149:200-208.［85］李心丹.抗菌肽O3TR,C12O3TR和Jelleine-Ⅰ对柑橘Penicillium digitatum的控制效果及机制研究［D］.重庆:西南大学,2020.［86］THERY T,O’CALLAGHAN Y,O’BRIEN N,et al.Optimisation of the antifungal potency of the amidated peptide H-Orn-Orn-Trp-Trp-NH2 against food contaminants［J］.International Journal of Food Microbiology,2018,265:40-48.［87］LIMA P G,FREITAS C D T,OLIVEIRA J T A,et al.Synthetic antimicrobial peptides control Penicillium digitatum infection in orange fruits［J］.Food Research International,2021,147:110582.［88］HERBEL V,SIEBER-FRANK J,WINK M.The antimicrobial peptide snakin-2 is upregulated in the defense response of tomatoes (Solanum lycopersicum) as part of the jasmonate-dependent signaling pathway［J］.Journal of Plant Physiology,2017,208:1-6.［89］辛艳丽,王静,杨浩杰,等.抗菌肽Hst5抑制串珠镰刀菌生长及作用机制研究［J］.食品安全质量检测学报,2022,13(22):7174-7182.［90］刘亚楠.Fengycin类抗菌脂肽的抗真菌机制研究［D］.南京:南京农业大学,2020.
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备注/Memo
第一作者简介：王耘（1985-），女，博士，副教授，现主要从事园艺产品品质与安全等研究工作。E-mail:wangyun@jit.edu.cn.责任作者：张长青（1974-），男，博士，教授，现主要从事蓝莓高品质种植等研究工作。E-mail:zcq@jit.edu.cn.基金项目：产教融合型一流课程资助项目；校级教育教改研究资助项目（JYJG202306）；学术拔尖人才培养工程资助项目。收稿日期：2025-01-01