XIA Xi,GONG Rui,LENG Hanbing,et al.Comprehensive Evaluation of Chlorophyll Fluorescence Response and Heat Tolerance of Different Azalea Cultivars to High-temperature Stress[J].Northern Horticulture,2025,(6):82-89.[doi:10.11937/bfyy.20243338]
高温胁迫下杜鹃花叶绿素荧光响应及耐热性评价
- Title:
- Comprehensive Evaluation of Chlorophyll Fluorescence Response and Heat Tolerance of Different Azalea Cultivars to High-temperature Stress
- 文章编号:
- 1001-0009(2025)06-0082-08
- 分类号:
- S 685.21
- 文献标志码:
- A
- 摘要:
- 以映山红亚属2个杂交组合的双亲及其子代为试材，采用人工模拟高温的试验方法，研究了高温胁迫(40 ℃)对杜鹃花形态和叶绿素荧光参数的影响，以期为揭示杜鹃花光合机构对高温的响应机制以及耐热品种的筛选提供参考依据。结果表明：高温胁迫致使杜鹃幼苗叶片的潜在光化学活性（Fv/Fo）、最大光化学效率（Fv/Fm）和光下PSⅡ实际光能转换效率（Fv′/Fm′）显著下降，变化幅度与胁迫强度成正比。高温胁迫下，非光化学淬灭系数（NPQ）在耐热品种中上升而在热敏感品种中下降。主成分分析显示，杂交组合1中的双亲和F1代“胭脂”具有很好的耐热性，其在受到高温胁迫后启动了热耗散机制适应环境高温。相关性分析综合评判得出7种杜鹃耐热性强弱顺序为“紫鹤”＞“红苹果”＞“胭脂”＞“白鹤”＞“艳秀”＞“粉红泡泡”＞“玉秀”，与其田间耐热性表现一致。“红苹果”和“胭脂”耐热性较强，可部分替代常规品种“紫鹤”应用于城市绿化。
- Abstract:
- Taking two hybrid combinations of Azalea，along with their parents and offspring as experimental materials,an artificially simulated high-temperature treatment (40 ℃) was employed to investigate the effects on the morphology and chlorophyll fluorescence parameters,in order to provide reference for revealing the response mechanism of photosynthetic apparatus to high temperature and screening of heat-resistant varieties of Azalea.The results showed that the contents of Fv/Fo，Fv/Fm and Fv′/Fm′ of Azalea seedlings were all declined significantly under high temperature stress，and the variation range increased linearly with the intensity of the stress.Under high temperature stress，NPQ increased in heat-resistant varieties and decreased in heat-sensitive varieties.Principal component analysis showed that both parents and progeny ‘Yanzhi’ in hybrid combination 1 had good heat resistance，and the heat dissipation mechanism was activated to adapt to high environmental temperature after being subjected to high temperature stress.The comprehensive evaluation of correlation analysis indicate that the heat tolerance sequence of seven varieties of azaleas was ‘Zihe’＞‘Hongpingguo’＞‘Yanzhi’＞‘Baihe’＞‘Yanxiu’＞‘Fenhongpaopao’＞‘Yuxiu’，in accordance with their behavior in the field.The cultivars with higher heat-tolerance were ‘Hongpingguo’ and ‘Yanzhi’ which could be applied to city landscape and partly substitute conventional cultivar ‘Zihe’.
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备注/Memo
第一作者简介：夏溪（1984-），女，硕士，高级工程师，现主要从事杜鹃花种质资源与遗传育种等研究工作。E-mail：xiaxi@shbg.org.责任作者：张春英（1971-），女，博士，教授级高级工程师，现主要从事园林植物资源与创新利用等研究工作。E-mail：zhangchunying@shbg.org.基金项目：上海市农业农村委员会科技攻关资助项目（2023-02-08-00-12-F04606）。收稿日期：2024-08-20