GAO Qi,ZHANG Debin,GAO Shaochen,et al.Effects of Foliar Spraying With Sodium Selenite on the Content and Quality of Active Ingredients in Honeysuckle[J].Northern Horticulture,2024,(18):92-99.[doi:10.11937/bfyy.20240652]
叶面喷施亚硒酸钠对金银花有效成分含量及品质的影响
- Title:
- Effects of Foliar Spraying With Sodium Selenite on the Content and Quality of Active Ingredients in Honeysuckle
- 文章编号:
- 1001-0009(2024)18-0092-08
- Keywords:
- honeysuckle; foliar spraying; sodium selenite; active ingredients
- 分类号:
- S 567.79
- 文献标志码:
- A
- 摘要:
- 以金银花为试材，对其进行叶面喷施亚硒酸钠，采用高效液相色谱法和紫外分光光度法，研究了外源硒对金银花叶片有效成分含量的影响，以期促进富硒金银花全草进一步综合利用，为金银花全草开发成食品或药品开拓更广阔的前景。结果表明：当亚硒酸钠浓度在10、20、50、100 mg·L-1时对金银花的生长起到促进作用；当亚硒酸钠浓度为10 mg·L-1时，绿原酸含量高达1.06%、可溶性糖含量高达1.51%、可溶性蛋白质含量为32.51 mg·g-1，较对照增加幅度分别为33.62%、57.29%、27.49%。当亚硒酸钠浓度为50 mg·L-1时，异绿原酸A、异绿原酸C、维生素C含量达到峰值分别为1.36%、0.95%、0.73 mg·g-1，此时浓度较对照分别增加了20.80%、4.58%、58.69%。当亚硒酸钠浓度为100 mg·L-1时，木犀草苷最大值为1.66%，与对照提高了40.19%。综上所述，适宜浓度的亚硒酸钠，对金银花的生长起到一定的促进作用，有助于金银花叶片中有效成分的合成与积累，从而提高金银花药食两用的品质。
- Abstract:
- Taking the honeysuckle as the experimental material，sodium selenite was sprayed on its leaves.High performance liquid chromatography and UV spectrophotometry were used，the effect of exogenous selenium on the content of effective components in honeysuckle leaves was studied,in order to promote the comprehensive utilization of selenium rich honeysuckle and explore broader prospects for the development of honeysuckle into food or medicine.The results showed that when the concentration of sodium selenite was within 10，20，50，and 100 mg·L-1，it promoted the growth of honeysuckle.When the concentration of sodium selenite was 10 mg·L-1，the content of chlorogenic acid was as high as 1.06%；the soluble sugar content was as high as 1.51%；the soluble protein content was 32.51 mg·g-1，with an increase of 33.62%，57.29%，and 27.49% compared to the CK，respectively.When the concentration of sodium selenite was 50 mg·L-1，the contents of isochlorogenic acid A，isochlorogenic acid C，and vitamin C reached their peak values of 1.36%，0.95%，and 0.73 mg·g-1，respectively.At this time，the concentration increased by 20.80%，4.58%，and 58.69% compared to the CK，respectively.When the concentration of sodium selenite was 100 mg·L-1，the maximum value of luteolin was 1.66%，which was 40.19% higher than the CK.In summary，the appropriate concentration of sodium selenite had a certain promoting effect on the growth of honeysuckle，which helped to synthesize and accumulate effective ingredients in honeysuckle leaves，thereby improving the quality of honeysuckle′s medicinal and edible dual-use.
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备注/Memo
第一作者简介：高琪（1998-），女，硕士研究生，研究方向为农产品加工及贮藏工程。E-mail：1932103278@qq.com.责任作者：谷春梅（1976-），女，博士，副教授，现主要从事农产品加工及营养调控等研究工作。 E-mail: jjnong2008@126.com.基金项目：国家自然科学基金资助项目（22007043）。收稿日期：2024-02-13