CHEN Bin,SUN Qiufang.Study on Bacteriostasis of Submerged Fermentation Products of Ganoderma applanatum[J].Northern Horticulture,2024,(10):99-105.[doi:10.11937/bfyy.20240352]
树舌灵芝深层发酵产物抑菌作用的研究
- Title:
- Study on Bacteriostasis of Submerged Fermentation Products of Ganoderma applanatum
- 文章编号:
- 1001-0009(2024)10-0099-07
- Keywords:
- Ganoderma applanatum; submerged fermentation; extract; bacteriostasis; minimum inhibitory concentration
- 分类号:
- S 567.31
- 文献标志码:
- A
- 摘要:
- 以分离自广西乐业天坑群的树舌灵芝的深层发酵产物（菌丝体、发酵液）为试材，采用滤纸片法、二倍稀释法，研究了树舌灵芝发酵产物提取物的抑菌作用，以期为开发防腐、食品保鲜产品提供参考依据。结果表明：树舌灵芝菌丝体提取物和发酵液提取物中均含有抗菌活性物质，发酵液乙酸乙酯提取物的抑菌能力最强，对10种供试菌均有较好的抑菌效果；发酵液乙酸乙酯提取物梯度洗脱、分离纯化后得到的30%乙酸乙酯组分（B5）的抑菌能力最高，对10种供试菌的抑菌圈直径基本上均最大；B5对藤黄微球菌的最低抑菌浓度（MIC）值为25 mg·mL-1，对大肠杆菌等5种菌的MIC值为50 mg·mL-1，对酿酒酵母的MIC值为200 mg·mL-1，对黑曲霉、米曲霉和绿色木霉的MIC值为400 mg·mL-1。
- Abstract:
- The antimicrobial activity of Ganoderma lucidum fermentation products (mycelium and fermentation broth) isolated from Leye Tiankeng Group in Guangxi was studied by using filter paper and double dilution method,in order to provide reference for developing anti-corrosion and food preservation products.The results showed that both mycelium extract and fermentation broth extract contained antibacterial active substances,and ethyl acetate extract of fermentation broth had the strongest antibacterial activity against all 10 tested microbes.The 30% ethyl acetate component (B5) of ethyl acetate extract from fermentation broth had the highest antibacterial activity,and the diameters of antibacterial zone were basically the largest.The minimum inhibitory concentration (MIC) value of B5 against Micrococcus luteus was 25 mg·mL-1,the MIC value for Escherichia coli,Bacillus subtilis,Proteus vulgaris,Staphylococcus aureus and Candida albicans were 50 mg·mL-1,the MIC value for Saccharomyces cerevisiae was 200 mg·mL-1,and the MIC value for Aspergillus niger,Aspergillus oryzae and Trichoderma viride was 400 mg·mL-1.
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备注/Memo
第一作者简介：谌斌（1967-），男，博士，研究员，现主要从事真菌资源的开发利用等研究工作。E-mail：binchen1967cn@126.com.基金项目：国家自然科学基金资助项目（41967019）；广西自然科学基金面上资助项目（2011GXNSFA018080）。收稿日期：2024-01-23