DU Bin.Mechanism of Nitric Oxide Signaling Pathway Regulating AsA-GSH Circulation of Pepper Under High Temperature Stress[J].Northern Horticulture,2024,(14):8-18.[doi:10.11937/bfyy.20240238]
高温胁迫下NO信号途径调控辣椒AsA-GSH循环的机制
- Title:
- Mechanism of Nitric Oxide Signaling Pathway Regulating AsA-GSH Circulation of Pepper Under High Temperature Stress
- 文章编号:
- 1001-0009(2024)14-0008-11
- Keywords:
- pepper; high temperature stress; NO; AsA-GSH circulatory
- 分类号:
- S 641.3
- 文献标志码:
- A
- 摘要:
- 以“新22号”尖椒为试材，采用高温（38±1）℃胁迫条件下，喷施不同浓度外源NO供体SNP、NO合成酶抑制剂、硝酸还原酶抑制剂和NO清除剂的方法，研究了各处理辣椒幼苗内源NO含量、活性氧（ROS）代谢、AsA-GSH循环系统的关键物质含量、关键酶活性及基因表达的影响，以期揭示高温胁迫下NO信号途径调控辣椒AsA-GSH 循环代谢机制。结果表明：高温胁迫可显著提高辣椒内源NO含量，NO清除剂和酶抑制剂可显著降低内源NO含量；添加适当浓度的外源NO可有效提高辣椒叶片抗坏血酸过氧化物酶（APX）、单脱氢抗坏血酸还原酶（MDHAR）、脱氢抗坏血酸还原酶（DHAR）和谷胱甘肽还原酶（GR）的活性，上调辣椒MDHAR、DHAR和GR基因的表达，提高AsA、脱氢抗坏血酸（DHA）、GSH、氧化型谷胱甘肽（GSSG）含量以及AsA/DHA和GSH/GSSG的比值，同时降低O·2产生速率和H2O2含量；添加NO清除剂和合成抑制剂则会逆转上述变化。综上，NO信号途径在辣椒响应高温胁迫的过程中起重要作用，NO信号分子可调控辣椒AsA-GSH循环系统关键基因的表达和翻译后修饰，有效提升关键酶的活性，提高AsA-GSH循环中还原态/氧化态的比例，从而维持体内ROS平衡。补充适量外源NO可有效提升辣椒对高温胁迫的抵抗能力，增强其耐热性。
- Abstract:
- Taking ‘Xinjianjiao 22’ pepper as the experimental material，using the method of spraying different concentrations of exogenous NO donor SNP，NO synthase inhibitor，nitrate reductase inhibitor and NO scavenger under high temperature stress (38±1)℃，the effects of endogenous NO content，ROS metabolism，key substances content，key enzyme activity and gene expression in AsA-GSH circulatory system of different treatments were studied, in order to reveal the mechanism by which the NO signaling pathway regulates the AsA-GSH cycle metabolism of pepper under high-temperature stress.The results showed that high temperature stress could significantly increased the endogenous NO content in pepper，while NO scavengers and enzyme inhibitors could significantly reduce the endogenous NO content.Adding appropriate concentration of exogenous NO could effectively increase the enzyme activities of ascorbate peroxidase (APX)，monodehydroascorbate reductase (MDHAR)，dehydroascorbate reductase (DHAR) and glutathione reductase (GR)，up-regulate the expression of MDHAR,DHAR and GR genes，and increase the levels of AsA，dehydroascorbic acid (DHA)，GSH and oxidized glutathione (GSSG).Adding NO scavenger and synthesis inhibitor would reverse the above changes.The results showed that NO signaling pathway played an important role in the response of pepper to high temperature stress，and NO signaling molecules could regulate the expression and post-translation modification of key genes in AsA-GSH circulation system of pepper，effectively enhance the activity of key enzymes，and increase the ratio of reduced state to oxidized state in AsA-GSH circulation，thus maintaining the balance of ROS in vivo.Supplementing appropriate exogenous NO could effectively improve the resistance of pepper to high temperature stress and enhance its heat resistance.
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备注/Memo
作者简介：杜宾（1980-），男，博士，副教授，现主要从事植物保护等研究工作。E-mail：dubin23@126.com.基金项目：山西省科技厅重点研发计划资助项目（201803D221004-2）。收稿日期：2024-01-16