LI Yanlin,HE Huaipeng,LIU Jing,et al.Analysis of Organic Acid Components in Root Exudates and Their Effects on Soil Nutrients and Enzyme Activities[J].Northern Horticulture,2024,(12):73-79.[doi:10.11937/bfyy.20234429]
不同根系分泌有机酸组分分析及其对土壤养分、酶活性的影响
- Title:
- Analysis of Organic Acid Components in Root Exudates and Their Effects on Soil Nutrients and Enzyme Activities
- 文章编号:
- 1001-0009(2024)12-0073-07
- Keywords:
- root exudates; organic acids; soil nutrients; soil enzyme activity
- 分类号:
- S 812.99
- 文献标志码:
- A
- 摘要:
- 以高寒草甸3种典型优势植物莎草科嵩草（Kobresia myosuroides (Villars) Foiri）、豆科异叶米口袋（Gueldenstaedtia diversifolia）和杂类草草玉梅（Anemone rivularis Buch.-Ham.）为研究对象，采用高效液相色谱分析其中的有机酸分布特征，并将检测到的有机酸组分添加到新鲜原位土壤中培养21 d后，研究有机酸对土壤中主要养分及酶活性的影响，以期了解根系分泌物有机酸调控土壤生产力的作用机制。结果表明：3种植物根系分泌物中共检出4种有机酸种类分别为乳酸、乙酸、柠檬酸和富马酸，乳酸在3种植物根系均有检出，浓度分别为7.02、13.25、4.84 mg·L-1；乙酸在嵩草和草玉梅中检出，浓度分别为19.32、7.61 mg·L-1；富马酸在嵩草和异叶米口袋中检出，浓度分别为0.32、0.39 mg·L-1；柠檬酸仅在嵩草中检出，浓度为5.24 mg·L-1。不同组分的有机酸均显著提高了土壤速效氮和速效磷含量，而且表现出不同的浓度效应（P＜0.05）。土壤脲酶、蔗糖酶和过氧化氢酶活性随乳酸和富马酸浓度的升高而升高，随乙酸浓度的升高而下降，除了高浓度（20 mg·L-1）乙酸对土壤过氧化氢酶活性有抑制作用，其他有机酸对土壤脲酶、蔗糖酶和过氧化氢酶的活性均表现出促进作用。研究表明，根系分泌物有机酸有利于提高土壤中主要养分的含量，对土壤酶活性有积极的促进作用，有利于提高土壤生产力。
- Abstract:
- K.pygmaea,Gueldenstaedtia diversifolia，and A.rivularis of the three typical plants in alpine meadow were selected as the research objects.The distribution characteristics of organic acids in the root exudates were analyzed by high performance liquid chromatography，and the detected organic acid components were added to the soil and cultivated for 21 days，and their effects on the main nutrients and enzyme activities in the soil were studied，in order to explore the organic acid of root exudates regulation mechanism of soil productivity.The results showed that lactic acid，acetic acid，citric acid and fumaric acid were detected in root exudates of three plants.Lactic acid was detected in the roots of three plants，with concentrations of 7.02，13.25,4.84 mg·L-1，respectively.Acetic acid was detected in K.pygmaea and A.rivularis，and the concentrations were 19.32,7.61 mg·L-1，respectively.Fumaric acid was detected in K.pygmaea and Gueldenstaedtia diversifolia，with concentrations of 0.32,0.39 mg·L-1 respectively.Only citric acid was detected in root exudates of K.pygmaea with concentrations of 5.24 mg·L-1.Different organic acids significantly increased the contents of available nitrogen and available phosphorus in soil，and showed different concentration effects (P＜0.05).The activities of soil urease，sucrase and catalase increased with the increase of lactic acid and fumaric acid concentration，and decreased with the increase of acetic acid concentration.Organic acids promoted the activities of soil urease，sucrase and catalase，except that high concentration (20 mg·L-1) of acetic acid inhibited the activity of soil catalase.The results showed that the organic acids in root exudates were beneficial to increase the content of main nutrients in soil，promote soil enzyme activity and improve soil productivity.
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备注/Memo
第一作者简介：李彦林（1990-），女，博士，副教授，现主要从事环境微生物学等研究工作。E-mail：ygzzfyhlyl@163.com.基金项目：国家自然科学基金青年科学基金资助项目(32101394)；重庆市教育委员会科学技术研究资助项目(KJQN202101319)。收稿日期：2023-12-19