MA Qingqing,JIAO Ziwei,SHEN Hongming,et al.Establishment of a Rapid Propagation System for in vitro Stem Segments of Lagerstroemia indica ‘Wuye’[J].Northern Horticulture,2024,(12):52-58.[doi:10.11937/bfyy.20233914]
‘午夜’紫薇离体茎段快繁体系的建立
- Title:
- Establishment of a Rapid Propagation System for in vitro Stem Segments of Lagerstroemia indica ‘Wuye’
- 文章编号:
- 1001-0009(2024)12-0052-07
- Keywords:
- Lagerstroemia indica ‘Wuye’; stem segments; axillary bud induction; proliferation; rooting
- 分类号:
- S 723.1
- 文献标志码:
- A
- 摘要:
- 以紫薇新品种‘午夜’带腋芽的幼嫩茎段为外植体，采用正交实验法，研究了NaClO与C2H5OH不同组合的消毒时间对外植体污染及萌发的影响,探讨WPM、1/2MS及MS 3种基本培养基和6-BA、NAA不同浓度组合对‘午夜’紫薇嫩茎腋芽诱导和增殖的影响以及在3种基本培养基上和不同IBA浓度组合对‘午夜’紫薇生根的影响，以期为‘午夜’紫薇大量繁殖提供参考依据。结果表明：75% C2H5OH消毒时间为30 s，5% NaClO消毒时间为6 min时，污染率较低，为18.6%；WPM培养基适合‘午夜’紫薇的茎段腋芽诱导与增殖，当6-BA、NAA浓度分别为1.0、0.5 mg·L-1时诱导率最高，达93.28%；当6-BA浓度为1.0 mg·L-1，NAA浓度为0.1 mg·L-1时的增殖系数最高，为5.83；IBA浓度变化对‘午夜’紫薇生根影响显著，在1/2MS培养基中添加0.5 mg·L-1 IBA时生根率最高，为92.99%，且无菌苗生长情况较好；炼苗后将组培苗移栽到泥炭土∶珍珠岩∶蛭石为3∶1∶2的基质上，成活率高达91%。
- Abstract:
- Taking the young stem segments with axillary buds of a new variety of Lagerstroemia indica ‘Wuye’ as explants，the orthogonal experimental method was used to study the effects of disinfection time of different combinations of NaClO and C2H5OH on the contamination and germination of the explants.The effects of three basic media，WPM，1/2MS，and MS，as well as different concentration combinations of 6-BA and NAA，on the induction and proliferation of axillary buds from tender stems of L.indica ‘Wuye’ were studied，as well as the effects of three basic media and different concentration combinations of IBA on the rooting of L.indica ‘Wuye’ were studied，in order to provide reference for mass propagation of Lagerstroemia indica ‘Wuye’.The results showed that,when the disinfection time of 75% C2H5OH was 30 seconds and the disinfection time of 5% NaClO was 6 minutes，the pollution rate was lower，at 18.6%.The WPM medium was suitable for the induction and proliferation of axillary buds in stem segments of L.indica ‘Wuye’.The highest induction rate was achieved when the concentrations of 6-BA and NAA were 1.0 mg·L-1 and 0.5 mg·L-1，respectively，reaching 93.28%.When the concentration of 6-BA was 1.0 mg·L-1 and the concentration of NAA was 0.1 mg·L-1，the highest proliferation coefficient was 5.83.The concentration change of IBA had a significant impact on the rooting of L.indica ‘Wuye’.Adding 0.5 mg·L-1 IBA to the 1/2MS had the highest rooting rate of 92.99%，and the growth of sterile seedlings was good.After refining the seedlings，transplanted the tissue culture seedlings onto a substrate of peat soil∶perlite∶vermiculite in a ratio of 3∶1∶2，with a survival rate of up to 91%.
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备注/Memo
第一作者简介：马清清（1999-），女，硕士研究生，研究方向为园林植物与观赏园艺。E-mail：maqingqing@stu.zafu.edu.cn.责任作者：顾翠花（1981-），女，博士，教授，现主要从事园林植物种质创新、遗传育种以及应用等研究工作。E-mail：gucuihua@zafu.edu.cn.基金项目：浙江省教育厅科研资助项目(Y202250160)；浙江省自然科学基金资助项目(LY21C160001)；浙江省农业新品种重大专项花卉育种专项资助项目(2021C02071-4)。收稿日期：2023-11-14