XU Zhiqing,LI Jie,LIN Ying,et al.Research on Isolation,Indentification and Biological Activity of Endophytic Fungi From Picrasma quassioides (D.Don) Benn.[J].Northern Horticulture,2024,(12):102-108.[doi:10.11937/bfyy.20233707]
苦木内生真菌的分离鉴定及生物活性研究
- Title:
- Research on Isolation,Indentification and Biological Activity of Endophytic Fungi From Picrasma quassioides (D.Don) Benn.
- 文章编号:
- 1001-0009(2024)12-0102-07
- Keywords:
- Picrasma quassioides (D.Don) Benn.; endophytic fungi; isolation and identification; bacteriostatic activity
- 分类号:
- S 567.19
- 文献标志码:
- A
- 摘要:
- 以苦木的根、茎、叶为试材，采用组织分离法从苦木的根、茎、叶中分离得到内生真菌，通过ITS序列分析，对其进行鉴定。采用K-B纸片法和最小抑制浓度法，研究了内生真菌发酵液对大肠杆菌和金黄色葡萄球菌的抑制效果，以期为苦木内生真菌在抗菌方面的研究价值提供参考依据，为寻找新型绿色高效的抗菌活性物质提供新思路。结果表明：苦木植物中蕴含丰富的内生真菌资源，从苦木根、枝、叶中分离得到16株内生真菌，分属于4门6纲8目8科8属，链格孢属（Ilternarsa）和葡萄座腔菌属（Botryosphaeria）为优势菌属。13株苦木内生真菌的发酵液提取物对2株指示菌表现出不同强度的抑菌作用，其中3株内生真菌的发酵液粗提物对大肠杆菌和金黄色葡萄球菌有较强抗菌活性，MIC值介于0.024~3.120 μg·mL-1。综合抑菌的广谱性和效能而言，G1菌株的抗菌效果最好。
- Abstract:
- Taking the roots，stems，and leaves of Picrasma quassioides (D.Don) Benn.as experimental materials，endophytic fungi were isolated from the roots，stems，and leaves of Picrasma quassioides (D.Don) Benn.using tissue isolation method.ITS sequence analysis was used to identify them.The inhibitory effects of endophytic fungi fermentation broth on Escherichia coli and Staphylococcus aureus were studied using K-B paper method and minimum inhibitory concentration method，in order to provide reference for the research value of endophytic fungi in the antibacterial of Picrasma quassioides (D.Don) Benn.and to provide new ideas for finding new green and efficient antibacterial active substances.The results showed that Picrasma quassioides (D.Don) Benn.plants contain abundant endophytic fungal resources.16 endophytic fungi were isolated from the roots，branches，and leaves of Picrasma quassioides (D.Don) Benn.belonging to 4 phyla，6 classes，8 orders，8 families，and 8 genera.The dominant genera were Ilternarsa and Botryospaeria. The fermentation broth extracts of 13 endophytic fungi from Picrasma quassioides (D.Don) Benn.showed varying degrees of antibacterial activity against two indicator bacteria.Among them，the crude extracts of fermentation broth from 3 endophytic fungi showed strong antibacterial activity against Escherichia coli and Staphylococcus aureus，with MIC values ranging from 0.02 to 3.12 μg·mL-1.In terms of comprehensive antibacterial broad-spectrum and efficacy，G1 strain has the best antibacterial effect.
参考文献/References:
［1］国家药典委员会.中华人民共和国药典［M］.北京:中国医药科技出版社,2020.［2］金永哲,谢丹萍,孙虎男.苦木提取物对耐药性肺癌细胞的促凋亡作用［J］.延边大学医学学报,2020,43(4):243-250.［3］刘亚群,张振霞,黄琼露,等.数据挖掘联合网络药理学探讨苦木抗肿瘤活性研究及验证［J］.韩山师范学院学报,2023,44(6):27-39.［4］洪聪慧,阿米乃,刘同,等.苦木组织培养与快速繁殖技术研究［J］.湖北民族大学学报(自然科学版),2023,41(3):281-285,307.［5］臧青民,黄华希,辛桂瑜,等.苦木提取物对肉鸡生长性能、屠宰性能、免疫功能及抗氧化性能的影响［J］.中国饲料,2023(15):76-80.［6］田丹梅，刘起林，高康，等. 中国省际食用菌产业集聚及空间差异化研究［J/OL］. (2023-10-01)［2024-01-23］.http://kns.cnki.net/kcms/detail/23.1247.s.20240119.1451.038.html.［7］王宁宁.三株植物内生真菌次级代谢产物的研究［D］.济南:山东大学,2020.［8］赵文娜,崔迎,白静,等.苦木提取物的体外抗菌活性和抗炎作用研究［J］.西北药学杂志,2019,34(4):505-508.［9］孙志良,卢向阳,刘自逵,等.白毛藤提取液成份定性分析及抑菌效果［J］.中兽医学杂志,2003(2):11-12.［10］林洋,孙梦桐,吕欣然,等.滤纸片法筛选抑制嗜水气单胞菌群体感应的乳酸菌及抑制作用分析［J］.食品科学,2018,39(14):125-131.［11］李旭东,马磊,张婷婷.羽毛针禾内生细菌分离鉴定及系统发育树分析［J］.北方园艺,2020(3):77-84.［12］杜君.植物内生真菌次级代谢产物抗菌活性的研究［D］.长春:吉林大学,2022.［13］蒋圆婷,陈文凤,田婧,等.半红树林植物黄槿内生真菌多样性及其抗菌活性研究［J］.安徽农业大学学报,2022,49(5):838-847.［14］殷娜,宋娜丽,普晓佳,等.民族药马利筋内生真菌生物活性研究［J］.广西植物,2022,42(5):781-789.［15］袁保红,杜青平,邓祖军.小连翘内生真菌种群分布及其抗菌性研究［J］.广东药学院学报,2007,23(3):307-311.［16］杨娟,董醇波,陈万浩,等.不同地区杜仲树皮内生真菌群落组成及生态功能结构的差异分析［J］.中国中药杂志,2019,44(6):1126-1134.
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备注/Memo
第一作者简介：徐芷晴（1996-），女，硕士研究生，研究方向为中药学。E-mail：953289297@qq.com.责任作者：徐凌川（1963-），男，本科，教授，现主要从事山东中医药大学教学与科研等工作。E-mail：xulingchuan 518@sina.com.基金项目：国家中医药管理局全国中药资源普查资助项目（GZY-KJS-2018-004）；国家中医公益性行业科研专项基金资助项目（201407002）；山东省中医药管理局中医药科技发展计划资助项目（2019-0976）。收稿日期：2023-10-28