LIN Yuyou,GUO Xiuwu,JIANG Chunguang,et al.Study on the Synthetic Change of Aroma 2-phenylethanol in ‘Vidal’ Grape and Its Bud Sport[J].Northern Horticulture,2024,(8):27-33.[doi:10.11937/bfyy.20233488]
‘威代尔’葡萄及其优良芽变果实香气物质
- Title:
- Study on the Synthetic Change of Aroma 2-phenylethanol in ‘Vidal’ Grape and Its Bud Sport
- 文章编号:
- 1001-0009(2024)08-0027-07
- Keywords:
- ‘Vidal’; grape; bud variant; 2-phenylethanol; AADC
- 分类号:
- S 663.1
- 文献标志码:
- A
- 摘要:
- 以‘威代尔’葡萄及其芽变果实为试材，采用氨基酸分析仪和荧光定量分析，研究了‘威代尔’葡萄及其芽变果实中的苯丙氨酸、苯乙胺、苯乙醛和2-苯乙醇动态含量，以及芳香族氨基酸脱羧酶（AADC）的动态相对表达量，以期明确‘威代尔’葡萄优良芽变果实特征香气2-苯乙醇合成变化，为生产提供优良酿造品种（系）原料。结果表明：‘威代尔’葡萄及其芽变果实中不同生育期苯丙氨酸、苯乙胺、苯乙醛和2-苯乙醇含量均呈现规律性变化，芽变果实中苯丙氨酸、苯乙胺、苯乙醛和2-苯乙醇含量均高于同期‘威代尔’果实。荧光定量结果表明，‘威代尔’及其果实中AADC的表达与2-苯乙醇含量均呈正相关。该研究发现的‘威代尔’葡萄芽变有望成为我国酿造优良葡萄酒重要的品种（系）原料。
- Abstract:
- Taking the ‘Vidal’ grape and its bud variant fruit as the test materials，and amino acid analyzer and fluorescence quantitative analysis were used，the dynamic content of phenylalanine，phenylethylamine，phenylacetaldehyde，and 2-phenylethanol in the ‘Vidal’ grape and its bud variant fruit were studied，as well as the dynamic relative expression of AADC enzyme，in order to clarify the synthesis changes the characteristic aroma of 2-phenylethanol in the excellent bud variant of the ‘Vidal’ grape，and the source were provided with raw materials for the production of excellent brewing varieties (series).The results showed that the content of phenylalanine，phenylacetamine，phenylacetaldehyde and 2-phenylethanol of the fruit was changed regularly in ‘Vidal’ grape and bud variant，and the content of phenylalanine，phenacetamine，phenylacetaldehyde and 2-phenylethanol in the ‘Vidal’ grape were higher than bud variant.The fluorescence quantification showed that AADC enzyme expression was positively correlated with 2-phenylethanol content in both ‘Vidal’ and bud variant.The bud variant of ‘Vidal’ was expected to provide an important variety (series) for the production of excellent wine in China.
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备注/Memo
第一作者简介：林玉友（1979-）,男，博士，副研究员，现主要从事葡萄果实品质调控等研究工作。E-mail：linyuyou@163.com.责任作者：郭修武（1959-），男，博士，教授，现主要从事葡萄遗传育种与分子生物学等研究工作。E-mail：guoxw1959@163.com.基金项目：国家现代葡萄产业技术体系岗位专家资助项目（CARS-29-yc-6）。收稿日期：2023-10-13