HUANG Xilun,TANG Tingting,JIN Rui,et al.Study on the Physiological Mechanism of Fruit Coloration of Panax japonicus[J].Northern Horticulture,2024,(6):100-107.[doi:10.11937/bfyy.20233292]
竹节参果实呈色生理机制研究
- Title:
- Study on the Physiological Mechanism of Fruit Coloration of Panax japonicus
- 文章编号:
- 1001-0009(2024)06-0100-08
- Keywords:
- Panax japonicus fruit; pigment content; anthocyanins; flavonoids; total phenol
- 文献标志码:
- A
- 摘要:
- 以5个不同呈色时期竹节参果实为试材，采用酶标仪检测法，研究了竹节参果实生长发育过程中叶绿素、类胡萝卜素、花色苷、类黄酮和总酚含量对果实呈色的影响,以期为揭示竹节参果实呈色生理机制提供参考依据。结果表明：果实、果皮叶绿素含量变化规律随果实转色呈逐渐降低趋势，果实绿果期含量最高1.060 mg·g-1；类胡萝卜素含量变化规律与叶绿素含量变化规律呈相反趋势，果皮成熟期含量最高0.234 mg·g-1；花色苷含量的变化规律与类胡萝卜素含量变化相似呈逐渐上升趋势，果皮成熟期含量最高3.393 mg·g-1。而果实总酚、类黄酮含量合成变化规律呈先升高后降低再升高的趋势，均是成熟时期果皮含量最高，总酚为2.790 mg·g-1，类黄酮为7.256 mg·g-1。因此，竹节参果实从绿果期进入转色期时，果实中的叶绿素逐渐被破坏，类胡萝卜素开始在果皮显现出来，花色苷也开始合成，总酚与类黄酮物质对竹节参果实呈色为正向调控作用。
- Abstract:
- Taking Panax japonicus fruits from 5 different colored periods as test materials,the effects of chlorophyll，carotenoids，anthocyanins，flavonoids，and total phenols content on the color of Panax japonicus fruit during growth and development were studied using an enzyme-linked immunosorbent assay (ELISA)，in order to provide reference for revealing the physiological mechanism of color formation in the fruit of Panax japonicus.The results showed that the chlorophyll content of fruit and peel decreased gradually with the color change of fruit，and the highest content was 1.060 mg·g-1 in the green fruit period.The change rule of carotenoid content was opposite to that of chlorophyll content，and the highest content was 0.234 mg·g-1 at the mature period of peel.The change rule of anthocyanin content was similar to that of carotenoid content，which showed a gradual upward trend，and the highest content was 3.393 mg·g-1 at the mature period of peel.The content of total phenol and flavonoids in the fruit increased first，then decreased and then increased again.The content of peel was the highest in the mature period，the total phenol was 2.790 mg·g-1，and the flavonoid was 7.256 mg·g-1.Therefore，when the fruit of P.japonicus enters the color-changing period from the green fruit period，the chlorophyll in the fruit was gradually destroyed，the carotenoids begin to appear in the peel，and the anthocyanins begin to synthesize.The total phenol and flavonoid substances had a positive regulatory effect on the color of the fruit of P.japonicus.
参考文献/References:
［1］国家药典委员会.中华人民共和国药典(一部)［M］.北京：中国医药科技出版社，2015.［2］孟艳,宋雪成,郝润璇,等.土家药复方竹节参片的研究进展［J］.中国民间疗法,2019,27(21):13-14.［3］鲁文杰,袁德培,曾楚华．竹节参作用的研究进展［J］．生物技术世界,2016,13(5):236．［4］马玲,谭德福．竹节参的研究概况［J］．时珍国医国药,2005,16(12):1306-1307．［5］MORITA T,TANAKA O,KOHDA H．Saponin composition of rhizomes of Panax japonicus collected in South Kyushu,Japan,and its significance in oriental traditional medicine［J］．Chemical and Pharmaceutical Bulletin,1985,33(9):3852-3858．［6］陈龙全,郜红利．复方竹节参片对大鼠佐剂性关节炎治疗作用的实验研究［J］．中国实验方剂学杂志,2003,9(3):25-26．［7］陈龙全,袁德培,孟卫星,等．复方竹节参片镇痛、抗炎作用的实验研究［J］．江苏中医,1998,30(9):47-48．［8］鄢丰,杨频．从竹节三七、肉桂、大黄中提纯的天然活性成分的镇痛作用研究［J］．中国新医药,2003，2(5):19-20．［9］陈龙全,陈朝俊．复方竹节参片抗风湿作用的免疫调节机制研究［J］．中国医药学报,2003,18(4):247-248．［11］刘家兰,李德清,刘红．竹节人参提取物对实验性心肌缺血的保护作用［J］．湖北民族学院学报(医学版),1999,16(1):6-8．［12］彭邦瑜,刘毅,邢宏志．中西医结合治疗Ⅱ型糖尿病94例［J］．湖北中医杂志,2001,23(4):17．［13］陈龙全,袁德培,刘红．复方竹节参片治疗类风湿性关节炎30例临床研究［J］．中医杂志,2003,44(5):362-363．［14］林先明,刘海华,郭杰,等．竹节参生物学特性研究［J］．中国野生植物资源,2007,26(1):5-7,38．［15］ZHU F Y,CHEN M X,CHAN W L,et al．SWATH-MS quantitative proteomic investigation of nitrogen starvation in Arabidopsis reveals new aspects of plant nitrogen stress responses［J］．Journal of Proteomics,2018,187:161-170．［16］WANG Y,GAO S,HE X,et al．Response of total phenols,flavonoids,minerals,and amino acids of four edible fern species to four shading treatments［J］．PeerJ,2020（8）:e8354．［17］郭咏梅．观赏辣椒果实呈色机制探析［D］．太谷:山西农业大学,2021．［18］黄春辉,高洁,张晓慧,等．黄肉猕猴桃果实发育期间色素变化及呈色分析［J］．果树学报,2014,31(4):617-623．［19］阮美颖,叶青静,周国治,等．不同颜色樱桃番茄果实成熟过程中色素的变化［J］．浙江农业科学,2013,54(5):526-528．［20］马晓华,陈春青,叶胜忠,等．不同花色紫薇花瓣的花色苷化合物组分及含量比较［J］．亚热带农业研究,2023,19(1):56-63．［21］谢倩,阳晓婷,杨薇,等．新品种紫辣椒和紫豇豆果皮花色苷含量测定［J］．江汉大学学报(自然科学版),2020,48(6):72-77．［22］张晓晓,黄午阳,於虹,等．不同种植地区蓝莓果中花色苷的分布［J］．中国食品学报,2022,22(10):314-324．［23］LI Y,LI H,WANG S,et al．Metabolomic and transcriptomic analyses of the flavonoid biosynthetic pathway in blueberry (Vaccinium spp．)［J］．Frontiers in Plant Science,2023（14）:1082245．［24］KARPPINEN K,LAFFERTY D J,ALBERT N W,et al．MYBA and MYBPA transcription factors co-regulate anthocyanin biosynthesis in blue-coloured berries［J］．The New Phytologist,2021,232(3):1350-1367．［25］周兰．苹果果实发育中类黄酮含量变化及相关基因的研究［D］．北京:中国农业科学院,2013．［26］刘晴晴,李勇,张明霞,等．紫色桑葚和白色桑葚总酚含量、抗氧化能力及代谢指纹图谱差异分析［J］．江苏农业学报,2022,38(3):813-820．［27］王俊青,张彦辉,易婉莹等.基于转录组测序红秋葵果实转色期花青素生物合成相关基因研究［J］.北方园艺，2022(14)：9-16.
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备注/Memo
第一作者简介：黄系伦（1995-），男，硕士研究生，研究方向为竹节参生理生态。E-mail:1938232158@qq.com.责任作者：张来（1977-），男，博士，教授，现主要从事竹节参次生代谢产物调控等研究工作。E-mail：975575681@qq.com.基金项目：国家自然科学基金资助项目(31660252)；贵州省科技厅科技资助项目（黔科合基础-ZK(2023)重点002）。收稿日期：2023-09-24