ZUO Min,LI Guangxin,ZUO Jingjing,et al.Plant Regeneration and Proliferation System Establish of Astragalus membranaceus[J].Northern Horticulture,2024,(6):93-100.[doi:10.11937/bfyy.20233377]
蒙古黄芪植株再生及增殖体系建立
- Title:
- Plant Regeneration and Proliferation System Establish of Astragalus membranaceus
- 文章编号:
- 1001-0009(2024)06-0093-08
- Keywords:
- Astragalus mongholicus; callus induction; regenerated plants; proliferation; germplasm resources
- 分类号:
- S 853.75
- 文献标志码:
- A
- 摘要:
- 以蒙古黄芪种子繁育而来的无菌苗获得的外植体(根、叶片、胚轴)为试材,通过设定不同浓度的生长调控激素6-BA、2,4-D、NAA,研究不同外植体在不同浓度激素的培养条件下对其愈伤组织诱导率、试管苗增殖及生根情况的影响,以期为蒙古黄芪不同组织外植体的愈伤组织诱导、植株分化及增殖培养条件提供参考依据。结果表明:以根、胚轴、叶片为外植体材料均可诱导愈伤组织产生,其中以胚轴为外植体为最优选择,愈伤组织诱导率最高达97.92%,最佳培养基为MS+2,4-D 1.0 mg·L-1+6-BA 0.5 mg·L-1+NAA 0.5 mg· L-1+蔗糖3.0%;试管苗增殖的最佳培养基为1/2MS+6-BA 1.0 mg·L-1+KT 0.5 mg·L-1+蔗糖3.0%,再生植株平均分化率最高为92.67%;生根培养时,最适宜的培养基为1/2MS+NAA 0.5 mg·L-1+白砂糖3.0%。该试验建立了蒙古黄芪植株再生及增殖体系,在黄芪生产实践中具有重要推广价值。
- Abstract:
- The root,leaf and hypocotyl of aseptic seedlings derived from Astragalus membranaceus seed were used as explants,and the growth-regulating hormones 6-BA,2,4-D,NAA were set at different concentrations,the effects of different explants on callus induction rate,in vitro plantlet increment and rooting were studied,in order to provide reference for callus induction,plant differentiation and proliferation of different tissue explants of Astragalus membranaceus.The results showed that root,hypocotyl and leaf were the best explants for callus induction,of which,hypocotyl was the best explants,with the highest callus induction rate of 97.92%,at 2,4-D 1.0 mg· L-1+6-BA 0.5 mg·L-1+NAA 0.5 mg·L-1+sucrose 3.0%,the best medium for plantlet proliferation at 1/2MS+6-BA 1.0 mg·L-1+KT 0.5 mg·L-1+sucrose 3.0%,the highest average differentiation rate of regenerated plantlets at 92.67%,and the optimum medium at 1/2MS+NAA 0.5 mg·L-1+sugar 3.0%.The system of plant regeneration and multiplication of Astragalus mongholicus was established in this experiment,which provided a powerful technical reference for its germplasm resources preservation,rapid seedling breeding and development,which has important popularizing value in the production practice of Astragalus mongholicus.
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备注/Memo
第一作者简介:左敏(1988-),女,硕士,助理研究员,现主要从事中药材栽培及新品种选育等研究工作。E-mail:zuomin8510@163.com.责任作者:李广信(1975-),男,博士,研究员,现主要从事中药材种质资源选育等研究工作。E-mail:nkylgx@163.com.基金项目:山西省基础研究计划青年科学研究资助项目(202303021212106);国家重点研发计划资助项目(2019YFC1710800);山西农业大学学术恢复科研专项资助项目(2020xshf05);山西省现代农业产业技术体系建设专项资金资助项目(2021-11);寿阳县科技重点研发计划资助项目(Y22002);山西省深度贫困县科技精准扶贫专项资助项目(2019FP-03)。收稿日期:2023-10-07