YANG Weihong,LIU Ruixia,YANG Xiaopen.Study on Physiological of Heat Resistance of Primula maximowiczii Treated With Calcium Spray[J].Northern Horticulture,2024,(9):47-53.[doi:10.11937/bfyy.20232577]
喷钙处理对胭脂花耐热反应的生理研究
- Title:
- Study on Physiological of Heat Resistance of Primula maximowiczii Treated With Calcium Spray
- 文章编号:
- 1001-0009(2024)09-0047-07
- Keywords:
- Primula maximowiczii; high temperature stress; physiological effect; induction of heat resistance
- 分类号:
- S 792.25
- 文献标志码:
- A
- 摘要:
- 以野生胭脂花（Primula maximowiczii）引种盆栽苗为试材，采用方差分析和广义线性模型，研究了胭脂花叶片生理生化指标，以期确定时间处理与喷钙处理共同作用对胭脂花叶片各个指标的影响程度。结果表明：1）在40 ℃高温胁迫下，随着胁迫时间的递增，胭脂花叶片相对电导率与丙二醛含量均呈增加趋势，但喷钙处理的上升速率均比清水处理缓慢；且3种喷钙处理中以10 mmol·L-1浓度的喷钙处理效果最为显著；2）在4 h高温胁迫下，超氧化物歧化酶（SOD）与过氧化物酶（POD）活性整体呈先增加后下降趋势，3种喷钙处理后的活性始终高于清水处理，并以10 mmol·L-1喷钙处理效果最为显著；3）在高温胁迫的整个过程中，可溶性蛋白质含量整体呈下降趋势，可溶性糖与游离脯氨酸含量整体呈上升趋势，但相比清水处理，喷钙后叶片中所含的渗透调节物质均增加，并以10 mmol·L-1浓度的喷钙处理效果显著。因此，经喷钙处理的胭脂花叶片各种耐热性生理生化指标均有所提高，故喷钙处理可提高胭脂花的耐热能力。
- Abstract:
- Taking wild Primula maximowiczii potted seedlings as experimental materials,variance analysis and generalized linear model were used to study the physiological and biochemical indicators of Primula maximowiczii leaves,in order to determine the degree of influence of time treatment and calcium spray treatment on various indicators of Primula leaves.The results showed that,1) under 40 ℃ high temperature stress，relative conductivity and malondialdehyde content in Primula maximowiczii leaves incrementally increased with prolonged stress.The rate of increase was more gradual with calcium spray treatment，notably with the 10 mmol·L-1 calcium spray，which exhibited the most significant effect.2) Following 4 hours of high temperature stress，SOD and POD activities initially increased，then decreased.These enzyme activities were consistently higher than in the clean water treatment，with the 10 mmol·L-1 calcium spray demonstrating the greatest impact.3) During the high temperature stress period，soluble protein content decreased，while soluble sugar and free proline content increased.Significantly，leaves treated with calcium spray，particularly the 10 mmol·L-1 concentration，showed a pronounced increase in osmotic adjustment substances compared to water treatment.Consequently，calcium spray treatment improved various heat resistant physiological and biochemical parameters in Primula maximowiczii leaves，suggesting enhanced heat tolerance in wild Primula maximowiczii.
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备注/Memo
第一作者简介：杨伟红（1979-），女，硕士，讲师，现主要从事园林植物生理生态等研究工作。E-mail：ywhlily@163.com.责任作者：杨晓盆（1961-），女，硕士，教授，现主要从事园林植物栽培与应用等研究工作。E-mail：yangxp296@sohu.com.基金项目：山西林业职业技术学院科研基金资助项目(20220107)；山西省林业重点研发计划专项资助项目（LYZD20210112）。收稿日期：2023-07-31