[1]张春雨,李小宇,王晨,等.百合斑驳病毒靖宇分离物全基因组序列测定与进化分析[J].北方园艺,2023,(24):59-64.[doi:10.11937/bfyy.20232243]
　ZHANG Chunyu,LI Xiaoyu,WANG Chen,et al.Sequencing and Analysis of the Genome of Lily mottle virus (LMoV) Jingyu Isolate[J].Northern Horticulture,2023,(24):59-64.[doi:10.11937/bfyy.20232243]
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百合斑驳病毒靖宇分离物全基因组序列测定与进化分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2023年24 页码: 59-64 栏目: 出版日期: 2023-12-30
Title:
Sequencing and Analysis of the Genome of Lily mottle virus (LMoV) Jingyu Isolate
文章编号:
1001-0009(2023)24-0059-06
作者:
张春雨1; 李小宇1; 王晨1; 黄瑞贤2; 黄淑敏2; 王永志1
（1.吉林省农业科学院（中国农业科技东北创新中心） 植物保护研究所，吉林 公主岭 136100；2.靖宇县农业技术推广中心，吉林 白山 135200）
Author(s):
ZHANG Chunyu1; LI Xiaoyu1; WANG Chen1; HUANG Ruixian2; HUANG Shumin2; WANG Yongzhi1
(1.Institute of Plant Protection，Jilin Academy of Agricultural Sciences（Northeast Agricultural Research Center of China），Gongzhuling，Jilin 136100；2.Jingyu Agricultural Technology Extension Center,Baishan，Jilin 135200)
关键词:
百合斑驳病毒; 基因组
Keywords:
Lily mottle virus（LMoV）; genome
分类号:
S 435.29
DOI:
10.11937/bfyy.20232243
文献标志码:
A
摘要:
以采自吉林省白山市靖宇县的野生百合感病叶片为试材，采用小RNA深度测序法检测到1株百合斑驳病毒（Lily mottle virus，LMoV），采用分段克隆方法，对LMoV靖宇分离物（LMoV-JY）的全基因组进行测定并分析，以期对吉林省百合病毒病的检测和防控提供参考依据。结果表明：LMoV-JY基因组大小为9 648个核苷酸（nt）序列（GenBank登录号MT795719），该核苷酸序列在第154~9 444位存在1个大的开放阅读框（ORF），编码1个多聚蛋白（分子量351.46 kD）。LMoV-JY与GenBank中登录的其他LMoV 分离物的全基因组核苷酸序列比较，其一致性为82.82%~97.85%，在氨基酸水平上的一致性为92.96%~98.64%，其中与百合斑驳病毒大连分离物LMoV-DL（HM222521）的一致性在该2种水平上均为最高。对比LMoV-JY外壳蛋白与其他54个分离物的氨基酸序列，发现可将LMoV分离物分为2个类群。
Abstract:
The infected leaves of wild lily were taken from Jingyu county，Baishan city，Jilin Province.Lily mottle virus (LMoV) was detected by small RNA deep sequencing and the whole genome of LMoV Baishan-Jingyu isolate (LMOV-JY) was determined and analyzed by segmentation cloning method，in order to provide reference for detection，prevention and control of lily virus disease in Jilin Province.The results showed that the genome of LMoV-JY consisted of 9 648 nucleotides (nt) (Genbank Accession No.MT795719).A large open reading frame (ORF) located at nt positions from 154 to 9 444 which encoding a polypeptide of 351.46 kD.By compairing with other isolates of LMoV，the nucleotide identity was from 82.82% to 97.85%，and the amino acid identity was from 92.96% to 98.64%，and it shared the highest identity with the Dalian isolate LMoV-DL (HM222521).Amino acid sequences comparison of the CP gene of LMoV-JY with other 54 isolates in GenBank showed that all of the isolates could be divided into two subgroups.

参考文献/References:

［1］KWON J Y，RYU K H，CHOI S H.Reverse transcription polymerase chain reaction-based system for simultaneous detection of multiple lily-infecting viruses［J］.The Plant Pathology Journal，2013,29(3):338-343.［2］李月月，孙健，谭冠林，等.百合斑驳病毒云南分离物全基因组序列分析及CP结构预测［J］.植物病理学报，2018,48(2):187-194.［3］ASJES C J，BLOM B G J，SCHADEWIJK A R.Effect of seasonal detection of Lily symptomless virus and Lily mottle virus on aphid-borne virus spread in Liliumin in the Netherlands［J］.Acta Horticulturae,2002,568：201-207.［4］刘博,明军,刘春,等.喇叭水仙中百合斑驳病毒的RT-PCR检测及序列分析［J］.园艺学报，2008,35(12):1843-1848.［5］杨丹,赵斌安,许燕,等.百合病毒检测技术研究进展［J］.生物学通报,2021,56(7):1-3.［6］徐榕雪,明军,穆鼎,等.百合三种病毒的多重RT-PCR检测［J］.园艺学报，2007,34(2):443-448.［7］袁志豪,李振锋,陆月霞,等.百合病毒的高通量测序鉴定和RT-PCR检测［J］.华中农业大学学报(自然科学版),2022,41(3):157-163.［8］周繇.长白山百合属植物野生资源及其开发利用［J］.中国野生植物资源，2002(3):22-23.［9］崔凯峰,刘丽杰,于长宝,等.长白山区野生百合引种驯化及园艺栽培技术［J］.中国野生植物资源，2013,32(3)：63-66.［10］李文琦,付崇毅,孙平平,等.四种主要草莓病毒研究进展［J］.北方园艺,2022(9):122-129.

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备注/Memo

第一作者简介：张春雨（1988-），女，硕士，助理研究员，现主要从事农业微生物等研究工作。E-mail：wawanice@126.com.责任作者：王永志（1978-），男，博士，研究员，现主要从事植物病理等研究工作。E-mail：yzwang@126.com.基金项目：吉林省农业科技创新工程资助项目（CXGC2021ZY022）。收稿日期：2023-07-04

更新日期/Last Update: 2024-01-09