[1]欧雪连,化青珠,庄泽岳,等.CaASR3基因沉默对辣椒抗旱性的影响[J].北方园艺,2023,(22):16-23.[doi:10.11937/bfyy.20231716]
　OU Xuelian,HUA Qingzhu,ZHUANG Zeyue,et al.Effects of CaASR3 Gene Silencing on Drought Resistance in Pepper[J].Northern Horticulture,2023,(22):16-23.[doi:10.11937/bfyy.20231716]
点击复制

CaASR3基因沉默对辣椒抗旱性的影响

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2023年22 页码: 16-23 栏目: 出版日期: 2023-11-30
Title:
Effects of CaASR3 Gene Silencing on Drought Resistance in Pepper
文章编号:
1001-0009(2023)22-0016-08
作者:
欧雪连1; 化青珠1; 庄泽岳1; 徐小万2; 吴智明1
（1.仲恺农业工程学院 园艺园林学院，广东 广州 510225；2.广东省农业科学院 蔬菜研究所，广东 广州 510640）
Author(s):
OU Xuelian1; HUA Qingzhu1; ZHUANG Zeyue1; XU Xiaowan2; WU Zhiming1
(1.College of Horticulture and Landscape Architecture,Zhongkai University of Agriculture and Engineering,Guangzhou,Guangdong 510225；2.Vegetable Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou,Guangdong 510640)
关键词:
辣椒; CaASR3; VIGS; 干旱
Keywords:
pepper; CaASR3; VIGS; drought
分类号:
S 641.3
DOI:
10.11937/bfyy.20231716
文献标志码:
A
摘要:
以‘遵辣1号’辣椒为试材，利用VIGS技术获得CaASR3基因沉默后植株，研究了CaASR3基因在干旱胁迫响应中的作用，以期为辣椒抗旱机理的研究提供分子基础。结果表明：基因沉默后，沉默植株CaASR3基因表达量在0、3、6、12 h显著低于对照植株；干旱处理后，CaASR3基因沉默植株叶片比对照植株失水萎蔫严重，过氧化物酶活性、脯氨酸含量、可溶性糖含量以及相对电导率显著上升，相对含水量显著降低，说明沉默CaASR3基因导致辣椒耐旱能力减弱。因此，CaASR3基因参与了辣椒抗旱调节途径。
Abstract:
Taking ‘Zunla-1’ pepper as test materials，the silencing of CaASR3 pepper plants was approached by using virus-induced gene silencing (VIGS) and the role of this gene in regulating responses to drought was investigated，in order to provide reference for further study of drought resistance mechanism in pepper.The results showed that the expression level of CaASR3 was significantly decreased following CaASR3 silencing at 0，3，6,12 hours.After drought treatment，in contrast to control plants，the leaves of CaASR3-silenced plants showed more water loss，peroxidase activity，proline content，soluble sugar content and relative conductivity increased significantly，and the relative water content decreased significantly，indicated that the CaASR3 silencing resulted in the attenuation of drought tolerance in pepper.Thus，the CaASR3 gene was involved in the regulation of drought resistance pathway in pepper.This study lay a foundation for improving drought tolerance of pepper in molecular breeding.

参考文献/References:

［1］邹学校,胡博文,熊程,等.中国辣椒育种60年回顾与展望［J］.园艺学报,2022,49(10):2099-2118.［2］邹学校,朱凡.辣椒的起源、进化与栽培历史［J］.园艺学报,2022,49(6):1371-1381.［3］蔡小桃,张颖,韦丽丽,等.辣椒ASR基因的克隆与表达分析［J］.南方农业学报,2021,52(11):3049-3058.［4］任伟.梭梭HaASR2在植物适应干旱生境中的作用初探［D］.兰州：兰州大学,2022.［5］孙聪,任鹏达,李连国，等.干旱胁迫下欧李叶片叶绿体光合参数与超弱光子辐射的关系［J］.北方园艺,2021(9):25-31.［6］许向阳,周博岩,刘文红,等.SLPIN6基因沉默对番茄抗旱性的影响［J］.东北农业大学学报,2022,53(1):16-23.［7］VIRLOUVET L，JACQUEMOT M P，GERENTES D，et al.The ZmASR1 protein influences branched-chain amio acid biosynthesis and maintains kernel yield in maize under water-limited conditions［J］.Plant Physiology，2011，157(2)：917-936.［8］JOO J，LEE Y H，KIM Y K，et al.Abiotic stress responsive rice ASR1 and ASR3 exhibit different tissue-dependent sugar and hormone-sensitivities［J］.Molecules and Cells，2013(35)：421-435.［9］ROM S，GILAD A，KALIFA Y，et al.Mapping the DNA-and zinc-binding domains of ASR1 (abscisic acid stress ripening)，an abiotic-stress regulated plant specific protein［J］.Biochimie，2006，88(6)：621-628.［10］HUANG K，ZHONG Y，LI Y，et al.Genome-wide identification and expression analysis of the apple ASR gene family in response to Alternaria alternata f.sp.mali［J］.Genome，2016，59(10)：866-878.［11］AKIR B，AGASSE A，GAILLARD C，et al.A grape ASR protein involved in sugar and abscisic acid signaling［J］.The Plant Cell，2003，15(9)：2165-2180.［12］邱鼎.小麦TaASR基因家族的鉴定与〖STBX〗TaASR1-D〖STBZ〗基因的抗逆功能研究［D］.武汉：华中科技大学,2021.［13］ZHANG L，YI X，GUO J，et al.Expression patterns of the ZmASR family during simulated drought stress in maize［J］.Agronomy Journal，2021，113(2)：894-905.［14］GAO H，LYU X，REN W，et al.〖STBX〗HaASR1 〖STBZ〗gene cloned from a desert shrub，Haloxylon ammodendron，confers drought tolerance in transgenic Arabidopsis thaliana［J］.Environmental and Experimental Botany，2020，180：104251.［15］YOOH J S，KIM J Y，LEE M B，et al.Over-expression of the Brachypodium ASR gene，BdASR4，enhances drought tolerance in Brachypodium distachyon［J］.Plant Cell Reports，2019，38：1109-1125.［16］史光珍,高鑫,朱新霞.〖STBX〗GbCDPK83〖STBZ〗基因在干旱胁迫下的功能鉴定［J］.西北农业学报,2022,31(4):441-447.［17］HU W，HUANG C，DENG X，et al.〖STBX〗TaASR1〖STBZ〗，a transcription factor gene in wheat，confers drought stress tolerance in transgenic tobacco［J］.Plant，Cell & Environment，2013，36(8):1449-1464.［18］IUSEM N D，BARTHOLOMEW D M，HITZ W D，et al.Tomato (Lycopersicon esculentum) transcript induced by water deficit and ripening［J］.Plant Physiology，1993，102(4)：1353.

相似文献/References:

[1]胡凤霞,唐艳领,刘金,等.辣椒砧木的筛选及其耐盐性研究[J].北方园艺,2013,37(04):24.
[2]陈 潇,盛萍萍,刘润进,等.西瓜与辣椒间作体系接种AM真菌对连作西瓜植株[J].北方园艺,2014,38(12):6.
　CHEN Xiao,SHENG Ping-ping,LIU Run-jin,et al.Effect of Intercropping System of Pepper-Watermelon Crops Inoculating Arbuscular Mycorrhizal Fungus on Growth and Defence Enzymes Activity of the Continuous Cropping Watermelon[J].Northern Horticulture,2014,38(22):6.
[3]张婷玉,林 多,杨延杰.辣椒根系分泌物的收集方法研究[J].北方园艺,2014,38(12):14.
　ZHANG Ting-yu,LIN Duo,YANG Yan-jie.Study on Collection Method of Root Exudates on Pepper[J].Northern Horticulture,2014,38(22):14.
[4]叶 林,李春江,张光弟,等.不同育苗穴数、基质配比和施肥量对温室辣椒幼苗生长及其秧苗质量的影响[J].北方园艺,2014,38(13):50.
　YE Lin,LI Chun-jiang,ZHANG Guang-di,et al.Effects of Different Seedling Number of Holes，Substrates and Fertilization on the Growth and Quality of Greenhouse Pepper Seedlings[J].Northern Horticulture,2014,38(22):50.
[5]孔小平,苗增建.等离子体处理辣椒种子对其性状及产量的影响[J].北方园艺,2013,37(13):34.
　KONG Xiao-ping,MIAO Zeng-jian.Effect of Plasma Treatment on Characters and Output of Pepper Seeds[J].Northern Horticulture,2013,37(22):34.
[6]高万里.白城地区辣椒产业发展对策研究[J].北方园艺,2014,38(04):159.
　GAO Wan-li.Study on the Development Countermeasure of Pepper Industry in Baicheng Area[J].Northern Horticulture,2014,38(22):159.
[7]岳振平.不同种植密度对早春大棚辣椒产量和产值的影响[J].北方园艺,2014,38(05):51.
　YUE Zhen-ping.Effect of Different Planting Densty on Fruit Yield and Output Value of Pepper in Early Spring[J].Northern Horticulture,2014,38(22):51.
[8]郭亚华,谢立波,王雪,等.辣椒经地面模拟诱变后的RAPD鉴定[J].北方园艺,2014,38(05):95.
　GUO Ya-hua,XIE Li-bo,WANG Xue,et al.The RAPD Detection of Capsicum annuum L. After Ground Simulation[J].Northern Horticulture,2014,38(22):95.
[9]杨飞,高艳明,李建设.宁夏设施沙培辣椒引种试验[J].北方园艺,2013,37(10):44.
　YANG Fei,GAO Yan-ming,LI Jian-she.Introduction Test of Sand Culture Facilities Pepper in Ningxia[J].Northern Horticulture,2013,37(22):44.
[10]马寿宾,孙艳,王琛,等.分子标记辅助选择辣椒抗疫病新种质研究[J].北方园艺,2013,37(10):107.
　MA Shou-bin,SUN Yan,WANG Chen,et al.Molecular Marker Assisted Selection of Pepper Materials with Resistance to Phytophthora Blight[J].Northern Horticulture,2013,37(22):107.

备注/Memo

第一作者简介：欧雪连（1997-），女，硕士研究生，研究方向为蔬菜遗传育种与生物技术。E-mail:1819965487@qq.com.责任作者：吴智明（1981-），男，博士，教授，硕士生导师，现主要从事蔬菜遗传育种与生物技术等研究工作。E-mail:18307763230@163.com.基金项目：国家自然科学基金资助项目（3207181037）;广东省重点建设学科科研能力提升资助项目（2021ZGJS004）。收稿日期：2023-05-18

更新日期/Last Update: 2023-12-14