CUI Huliang,JIA Jing,CAO Ruji.Variance Analysis of Petal Color Phenotypic and Flavonoids in Different Daylily Varieties[J].Northern Horticulture,2023,(23):68-74.[doi:10.11937/bfyy.20230095]
不同萱草品种花色表观特征与类黄酮物质的差异分析
- Title:
- Variance Analysis of Petal Color Phenotypic and Flavonoids in Different Daylily Varieties
- 文章编号:
- 1001-0009(2023)23-0068-07
- Keywords:
- Hemerocallis; petal color; anthocyanin; flavonoids; petal limb
- 分类号:
- S 682.2
- 文献标志码:
- A
- 摘要:
- 以萱草（Hemerocallis）5个色系的19个萱草品种为试材，采用色差仪测定花瓣檐部的Lab数值，采用HPLC测定其类黄酮物质，然后比较分析不同色系之间的花色差异，以期揭示不同萱草品种花色差异的物质基础。结果表明:黄色品种明度（L*）和黄度（b*）最高，紫色品种明度（L*）和黄度（b*）最低，红色品种的红度（a*）最高但明度（L*）和黄度（b*）较低。萱草主要的黄酮类物质为矢车菊素衍生物、槲皮素衍生物、异鼠李素 3-芸香糖苷和芦丁等物质；其中，黄色和粉色品种槲皮素衍生物、异鼠李素 3-芸香糖苷和芦丁等物质相对含量较高，橙色、红色和紫色品种的矢车菊素 3，5-葡萄糖苷、矢车菊素 3-芸香糖苷、槲皮素 7-葡萄糖苷、槲皮素 3-阿拉伯糖苷的相对含量较高。综上所述，萱草不同色系品种在明度（L*）、红度（a*）和黄度（b*）方面存在显著差异；黄色和粉色品种的槲皮素衍生物、异鼠李素 3-芸香糖苷和芦丁等黄酮类物质相对含量较高而矢车菊素较低，橙色、红色和紫色品种的矢车菊素衍生物和槲皮素衍生物相对含量较高。
- Abstract:
- A total of 19 Hemerocallis cultivars of 5 different color systems were chosen as experimental materials，and determined CIELab via colorimeter and flavonoids via HPLC in petal limb，in order to reveal the differences of the main color substances among different daylily cultivars.The results showed that，yellow varieties had the highest L* and b*，at the same time，purple cultivars had the lowest L* and b*.However，red cultivars had the highest a* but lower value of L* and b*，respectively.The main flavonoids were cyanidin derivatives，quercetin derivatives，isorhamnetin 3-rutoside and rutin.The relative contents of quercetin derivatives，isorhamnetin 3-rutoside and rutin in yellow and pink varieties were relatively higher than others；meanwhile the relative contents of cyanidin 3,5-glucoside，cyanidin 3-rutinoside，quercetin 7-glucoside and quercetin 3-arabinoside in orange，red and purple varieties were relatively higher.In conclusion，it showed significant difference in aspect of L*，a* and b* among different varieties；the yellow and pink varieties had the higher relative content flavonoids such as quercetin derivatives，isorhamnetin 3-rutoside and rutin，however，the main flavonoids in orange，red and purple varieties were cyanidin derivatives and quercetin derivatives.
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备注/Memo
第一作者简介：崔虎亮（1986-），男，博士，讲师，现主要从事园林植物应用等研究工作。E-mail：cuihuliang2005@126.com.基金项目：山西省基础研究计划项目青年科学研究资助项目（202103021223117）；山西省高等院校科技创新资助项目（2021L016）；太原理工大学校级基金面上资助项目（2022MS004）。收稿日期：2023-01-09