ZHANG Guoli,ZHANG Mengjie,HE Jindi,et al.Spatiotemporal Expression Pattern Analysis of Tulipa gesneriana L. HKT1 Gene Involved in Salt Stress Response[J].Northern Horticulture,2023,(16):71-77.[doi:10.11937/bfyy.20225133]
郁金香HKT1基因参与盐胁迫响应的时空表达模式分析
- Title:
- Spatiotemporal Expression Pattern Analysis of Tulipa gesneriana L. HKT1 Gene Involved in Salt Stress Response
- 文献标志码:
- A
- 摘要:
- 以郁金香盐敏感品种‘范思哲’为试材，通过水培试验分别设置胁迫处理（0.4 mol·L-1 NaCl）、对照（0 mol·L-1 NaCl），于胁迫后的0、4、8、12、24、48、72 h分别对郁金香的不同组织部位（根、鳞茎、地上茎、叶）进行取样，利用实时荧光定量PCR对4个组织在7个时间的HKT1基因时空差异表达水平进行分析，以期为探究HKT1基因在郁金香体内维持钾钠离子平衡的能力，进一步挖掘郁金香耐盐基因、完善郁金香耐盐机理提供参考依据。结果表明：郁金香在NaCl诱导下，随着盐胁迫的持续，HKT1基因在郁金香各组织 中均有上调，但表达丰度不同，说明HKT1基因在各组织的响应进程存在差异，是郁金香盐胁迫过程 中的差异表达基因。伴随着胁迫时间的增加，HKT1基因表达量在鳞茎、地上茎、叶组织 中均呈现先上升后下降的趋势，而在根 中通过持续高表达来维持体内钾钠离子的平衡，是郁金香响应盐胁迫最为敏感部位。HKT1基因是郁金香抵御盐胁迫相关的转录因子，其参与了郁金香对盐胁迫的调控过程。该研究发现郁金香HKT1基因时空表达模式,表明其在钾钠离子的选择性吸收过程 中发挥着重要的作用，该研究结果可为后续郁金香耐盐机制的研究奠定基础,提供分子依据和有效的基因资源。
- Abstract:
- Tulip salt sensitive variety ‘Versace’ was used as material,the stress group(0.4 mol·L-1 NaCl) and the control group (0 mol·L-1 NaCl) were set up through hydroponic culture test.Different tissue parts (root，bulb，aboveground stem，leaf) of tulip were sampled at 0，4，8，12，24，48，72 hours after stress.Real-time fluorescent quantitative PCR was used to analyze the spatiotemporal differential expression level of HKT1 gene expression in four tissues at seven times，in order to explore the ability of the HKT1 gene to maintain potassium and sodium ion balance in tulip，further excavate tulip salt tolerance gene and improve tulip salt tolerance mechanism.The results showed that under the induction of NaCl factors，with the continuation of salt stress，HKT1 gene was expressed in all tulip tissues，but the expression abundance was different，indicating that there were differences in the response process of HKT1 gene in various tissues，which was a differential expression gene in the process of tulip salt stress.With the increase of stress time，the expression of HKT1 gene increased first and then decreased in bulbs，aboveground stems and leaves，while maintaining the balance of potassium and sodium ions in the root through continuous high expression is the most sensitive part of tulip in response to salt stress.HKT1 gene was a transcription factor related to the resistance of tulip to salt stress，which was involved in the regulation of tulip to salt stress.The temporal and spatial expression pattern of tulip HKT1 gene obtained in this study showed that it played an important role in the selective absorption of potassium and sodium ions.The research conclusion could provide molecular evidence and effective gene resources for the subsequent study of the of the salt tolerance mechanism of tulip.
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备注/Memo
第一作者简介：张国莉（2000-），女，硕士研究生，研究方向为观赏植物栽培生理。E-mail：997635206@qq.com.责任作者：巨秀婷（1987-），女，硕士，副教授，现主要从事园林植物栽培与观赏植物育种等研究工作。E-mail：juxiuting@163.com.基金项目： 中国科学院2021年度“西部之光”人才培养计划资助项目(青科发规[2022]9号)；青海省自然科学基金资助项目（2020-ZJ-937Q）。收稿日期：2022-12-15