ZHOU Chenli,GUO Ting,JI Guangyan,et al.Identification and Analysis of the Transcription Factor and Laccase Expression Regulators Based on Genome and Transcriptome Databases in Phlebopus portentosus[J].Northern Horticulture,2023,(16):108-116.[doi:10.11937/bfyy.20224857]
基于基因组和转录组挖掘暗褐网柄牛肝菌转录因子以及漆酶表达调控因子
- Title:
- Identification and Analysis of the Transcription Factor and Laccase Expression Regulators Based on Genome and Transcriptome Databases in Phlebopus portentosus
- 关键词:
- 组学; 转录因子; 漆酶; 加权基因共表达调控网络
- 文献标志码:
- A
- 摘要:
- 以暗褐网柄牛肝菌（Phlebopus portentosus）为试材，采用基因组测序和转录组分析方法，研究转录因子类型、不同发育阶段的表达模式以及对漆酶的调控作用，以期为暗褐网柄牛肝菌子实体发育调控机制研究提供参考依据。结果表明：在暗褐网柄牛肝菌基因组 中共预测得到140个转录因子编码基因，分属于10个不同家族，C2H2型转录因子最多，其次为C3H型，这些转录因子参与调控催化活性、生物活性以及代谢过程。转录因子在菌丝体阶段、原基阶段以及采收阶段的表达模式存在差异性，调控不同的生物过程。通过基因组注释信息以及结构域的解析发现了6个漆酶基因，根据不同发育阶段的表达情况，分为4个不同的家族：始终不表达、菌丝体时期高表达、原基子实体时期高表达、稳定表达。有6个转录因子对4个漆酶基因起到了调控作用，其 中SRF型转录因子控制了菌丝体时期高表达的漆酶基因，具有正相关的关系，Sox1型转录因子控制了原基子实体漆酶的表达，呈负相关。转录因子和漆酶在子实体发育过程 中均起到了一定的调控作用。
- Abstract:
- Phlebopus portentosus was used as the test materials.Genome and transcriptome were conducted to identify transcription factors，expression patterns at different developmental stages，and regulatory effects on laccases，in order to provide reference for the research on the regulation mechanism of the fruiting body development of Phlebopus portentosus.The results showed that a total of 144 transcription factor were predicted in the genome，belonging to 10 different families.The C2H2 transcription factors were the most abundant，followed by C3H transcription factors，which were involved in the regulation of catalytic activity，biological and metabolic process.The expression patterns of transcription factors in mycelium stage，primordial stage and harvest stage were different，regulating different biological processes.Six laccase genes were detected through genome annotation and domain analysis.According to the expression at three different development stages，they were divided into four different families，never expressed，highly expressed at the mycelium stage，highly expressed at the primordia and fruiting body stage and stably expressed.There were 6 transcription factors that regulate 4 laccase genes，of which SRF transcription factors control the highly expressed laccase genes at the mycelium stage with positive correlation，Sox1 transcription factors control the expression of laccase in the primordial fruiting body with negative correlation.Transcription factors and laccase played a certain role in the regulation of fruiting body development.
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备注/Memo
第一作者简介：周陈力（1985-），女，硕士，助理研究员，现主要从事食用菌遗传育种等研究工作。E-mail：794357677@qq.com.责任作者：杨瑞恒（1983-），男，博士，副研究员，现主要从事食用菌遗传育种等研究工作。E-mail：yangruiheng@126.com.基金项目：上海市“科技创新行动计划”资助项目(20310741800)；国家自然科学基金资助项目(32072644)。收稿日期：2022-11-28