WEI Liqin,CHONG Peifang,BAO Xinguang.Flavonoids in Roses of Different Colors and Their Relationship With Flower Color[J].Northern Horticulture,2023,(09):37-46.[doi:10.11937/bfyy.20223855]
不同花色玫瑰类黄酮成分及其与花色关系
- Title:
- Flavonoids in Roses of Different Colors and Their Relationship With Flower Color
- 关键词:
- 花色; 类黄酮; 玫瑰; 超高效液相色谱-四极杆-飞行时间质谱(UPLC-Q-TOF-MS)
- Keywords:
- flower color; flavonoids; Rosa rugosa Thunb; ultra-performance liquid chromatography quadrupole-time-of-flight mass spectrometry
- 文献标志码:
- A
- 摘要:
- 以‘苦水玫瑰’‘墨红玫瑰’和‘保加利亚白玫瑰’3个不同花色的玫瑰为试材，采用CIE L*a*b*表色系法测量玫瑰花色，用超高效液相色谱-四级杆-飞行时间质谱(UPLC-Q-TOF-MS)联用技术定性定量分析玫瑰花中类黄酮化合物成分与含量，运用多元线性回归方法，研究玫瑰花瓣中类黄酮成分对其花色的影响，以期为玫瑰花色形成的物质基础提供参考依据。结果表明：3种不同花色玫瑰中共检测到58种类黄酮成分，其中花青苷只有1种，为矢车菊-3-O葡萄糖苷，约占30.45%；黄酮类有24种，约占18.85%；黄酮醇类9种，约占31.89%；黄烷醇类有8种，二氢黄酮类有6种，异黄酮类有3种，查尔酮有2种，其它及多酚类物质有5种；3种不同花色玫瑰中的黄酮和黄酮醇类化合物的含量不同，‘保加利亚白玫瑰’花瓣中的占其类黄酮总量的93.85%，而‘苦水玫瑰’花瓣和‘墨红玫瑰’花瓣中的分别占其类黄酮总量的29.33%和44.08%；多元线性回归分析显示儿茶素和乔松素是影响花色的最主要因素，其含量积累会影响参数L*、a*、C*值和h°，含量越高花色越偏向于红色。因此，矢车菊素是‘墨红玫瑰’和‘苦水玫瑰’呈色的主要物质，儿茶素和乔松素是影响‘苦水玫瑰’和‘墨红玫瑰’花色的色素成分，而黄酮和黄酮醇类化合物对‘保加利亚白玫瑰’的花色影响很大，其中的木犀草素和槲皮素-3-O葡萄糖苷是影响‘保加利亚白玫瑰’花色的色素成分。
- Abstract:
- Three kinds of roses with different flower colors，Rosa rugosa ‘Kushui’，Rosa ‘Crimson Glory’ and Rosa alba were used as experimental materials,the flower color of roses was measured by CIE L* a* b* color system method.The composition and content of flavonoids in roses were qualitatively and quantitatively analyzed by ultra-high performance liquid chromatography-quadrupole-time of flight mass spectrometry (UPLC-Q-TOF-MS).The effects of flavonoids in rose petals on their color were studied by multiple linear regression method，in order to provide reference for the material basis of rose color formation.The results showed that a total of 58 type of flavonoid components were detected in three different color roses，of which only one anthocyanin was cyanidin-3-O-glucoside，accounting for 30.45%.There were 24 kinds of flavonoids,accounting for 18.85%,9 kinds of flavonols，accounting for about 31.89%;there were 8 kinds of flavanols,6 kinds of dihydroflavones,3 kinds of isoflavones,2 kinds of chalcones,and 5 kinds of other and polyphenols.The contents of flavonoids and flavonols in three different color roses were different.The content of flavonoids in Rosa alba petals accounted for 93.85% of the total flavonoids,while the content of flavonoids in Rosa rugosa ‘Kushui’ petals and Rosa ‘Crimson Glory’ petals accounted for 29.33% and 44.08% of the total flavonoids，respectively.Multiple linear regression analysis showed that catechin and pinocembrin were the most important factors affecting flower color，and their contents accumulation would affect the parameters L*,a*,C* and h°.The higher the content，the more inclined to red color.In conclusion,cyanidin may be the main substance for the coloration of Rosa ‘Crimson Glory’ and Rosa rugosa ‘Kushui’.Catechin and pinocembrin were the pigment components that affect the color of Rosa ‘Crimson Glory’ and Rosa rugosa ‘Kushui’.Flavonoids and flavonols had a great influence on the color of Rosa alba.Luteolin and quercetin-3-O glucoside were the pigment components that affect the color of Rosa alba.
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备注/Memo
第一作者简介：魏丽琴（1996-），女，硕士研究生，研究方向为园林植物与应用。E-mail：2570766505@qq.com.责任作者：种培芳（1977-），女，博士，教授，现主要从事园林植物及荒漠植物生理生态等研究工作。E-mail：zhongpf@gsau.edu.cn.基金项目：甘肃省自然科学基金资助项目（20JR5RA035）；2023年高校研究生“创新之星”资助项目（2023CXZX-643）。收稿日期：2022-09-19