LIU Ye,MO Shilun,XIANG Liqing,et al.Salt Tolerance Evaluation of Different Germplasms of Andrographis paniculata During Germinating[J].Northern Horticulture,2023,(04):101-110.[doi:10.11937/bfyy.20221653]
不同种质穿心莲萌发期耐盐性评价
- Title:
- Salt Tolerance Evaluation of Different Germplasms of Andrographis paniculata During Germinating
- Keywords:
- Andrographis paniculata; seed germination; salt tolerance
- 文献标志码:
- A
- 摘要:
- 以10份不同产地来源的穿心莲种子为试材，采用培养皿纸上发芽法模拟盐胁迫环境，研究了NaCl胁迫对不同种质穿心莲萌发特性的影响，结合隶属函数法和系统聚类分析法对其耐盐性进行综合评价，以期为穿心莲耐盐种质筛选、种植和推广提供参考依据。结果表明：盐胁迫对各穿心莲种质种子的发芽率、发芽势、相对盐害率、可溶性蛋白质含量、过氧化氢酶（CAT）活性、脯氨酸（Pro）含量、种子活力均有显著影响，但不同种质之间响应情况不同；穿心莲在萌发期表现出一定耐盐性，不同种质穿心莲之间的耐盐性强弱不同，耐盐性综合评价将其分成了耐盐性较强、耐盐性中等、敏感3种类型,种质A（广西玉林）属于强耐盐；种质I（福建漳州）、种质E（广东湛江常规种）等属于中耐盐；种质F（广东湛江大叶种）属于盐敏感。
- Abstract:
- Ten Andrographis paniculata seeds of different origins were used as experimental materials，and the effects of NaCl stress on the germination characteristics of different germplasms of Andrographis paniculata were studied by simulating the salt stress environment using the method of germination on dish paper，and their salt tolerance was comprehensively evaluated by combining the affiliation function method and systematic cluster analysis，in order to provide reference for the screening，planting and promotion.The results showed that salt stress had significant effects on the seed germination rate，germination potential，relative salt damge rate，soluble protein content，catalase (CAT) activity，proline (Pro) content，and seed vigor of different germplasms of Andrographis paniculata，but different germplasms responded differently.Andrographis paniculata exhibited salt tolerance during the germination period.The comprehensive evaluation of salt tolerance classified them into three types,strongly salt-tolerant，moderately salt-tolerant，and sensitive.Germplasm A (Yulin，Guangxi) being strongly salt-tolerant；germplasm I (Zhangzhou，Fujian)，E (Zhanjiang conventional species，Guangdong) et al.being moderately salt-tolerant；germplasm F (Zhanjiang，Guangdong，large-leaved species) being sensitive.
参考文献/References:
[1]国家药典委员会.中华人民共和国药典.一部[S].北京：中国医药科技出版社，2020．[2]KIM N，LERTNIMITPHUN P，JIANG Y，et al.Andrographolide inhibits inflammatory responses in LPS-stimulated macrophages and murine acute colitis through activating AMPK[J].Biochemical Pharmacology,2019,170:113646.[3]LI X，YUAN K，ZHU Q，et al.Andrographolide ameliorates rheumatoid arthritis by regulating the apoptosis-netosis balance of neutrophils[J].International Journal of Molecular Sciences,2019,20(20):5035.[4]YUE G G L，LI L，LEE J K M，et al.Multiple modulatory activities of Andrographis paniculata on immune responses and xenograft growth in esophageal cancer preclinical models[J].Phytomedicine,2019,60:152886.[5]鹿蓓蓓,傅鹤秀,OKRAH E A,等.穿心莲内酯对小鼠乳腺癌的抑制作用和机制[J].中国药理学通报,2022,38(1):26-32.[6]PAEMANEE A，HITAKARUN A，WINTACHAI P，et al.A proteomic analysis of the anti-dengue virus activity of andrographolide[J].Biomedicine & Pharmacotherapy,2019,109:322-332.[7]ISLAM M T.Andrographolide，a new hope in the prevention and treatment of metabolic syndrome[J].Frontiers in Pharmacology,2017(8):571.[8]WONG S K，CHIN K Y，IMA-NIRWANA S.A review on the molecular basis underlying the protective effects of Andrographis paniculata and andrographolide against myocardial injury[J].Drug Design，Development and Therapy,2021(15):4615-4632.[9]邵艳华.穿心莲种质资源及其质量评价研究[D].广州:广州中医药大学,2015.[10]杨真,王宝山.中国盐渍土资源现状及改良利用对策[J].山东农业科学,2015,47(4):125-130.[11]杨劲松.中国盐渍土研究的发展历程与展望[J].土壤学报,2008(5):837-845.[12]陈娟,谷巍,段金廒,等.穿心莲生理特性及盐胁迫对其影响研究[J].中药材,2014,37(8):1322-1327.[13]刘贵河,郭郁频,任永霞,等.PEG胁迫下5种牧草饲料作物种子萌发期的抗旱性研究[J].种子,2013,32(1):15-19.[14]张瑀茜,高山,张锐,等.盐胁迫对不同油菜种子萌发的影响[J].种子,2021,40(1):94-98.[15]张金龙,陈宏亮,王鹏山,等.不同盐胁迫对华蒲公英种子萌发的影响[J].中国野生植物资源,2021,40(2):10-14.[16]童家赟.空气等离子体预处理提高穿心莲种子活力的研究[D].广州:广州中医药大学,2012.[17]海妮肯?山台,吴天忠,布勒马斯?三台,等.NaCl胁迫对三个葡萄品种幼苗的生理响应及其耐盐性综合评价[J].北方园艺,2022(1):16-23.[18]刘雅岚,代飞飞,吴柳,等.5种热带林木种子生活力染色最佳条件[J].海南师范大学学报(自然科学版),2018,31(4):441-447.[19]姚超,杨敏婕,许梅花.盐胁迫下玉米种子萌发及影响[J].农家参谋,2021(8):61-62.[20]董梦宇,徐立人,李彦慧.盐胁迫对糖芥种子萌芽及幼苗生长的影响[J].云南农业大学学报(自然科学版),2021,36(5):745-752.[21]贾海凤,张海艳.外源NO对NaCl胁迫下板蓝根种子萌发和幼苗生理特性的影响[J].中草药,2014,45(1):118-124.[22]齐琪,马书荣,徐维东.盐胁迫对植物生长的影响及耐盐生理机制研究进展[J].分子植物育种,2020,18(8):2741-2746.[23]SHAO Y H，GAO J L，WU X W，et al.Effect of salt treatment on growth，isoenzymes and metabolites of Andrographis paniculata(Burm.f.) Nees[J].Acta Physiol Plant,2015,37:35.[24]巩志勇.香椿种子萌发及幼苗生长对盐碱胁迫的响应[D].泰安:山东农业大学,2021.[25]马琳,张舒娜,张亚玉.东北延胡索种子萌发过程中生理生化变化[J].种子,2018,37(6):40-42.[26]黄雅丽,刘幸红,黄雯佳,等.盐分胁迫对景天三七幼苗生理生化特性的影响[J].中国农学通报,2021,37(10):42-47.[27]王中玉,逯昀,陈昆.NaCl胁迫对西瓜幼苗叶绿素荧光、光合特性、渗透调节及酶活性的影响[J].山西农业科学,2020,48(12):1909-1912.[28]丁小涛,何立中,张红梅,等.不同浓度盐胁迫对甜椒生长及生理特性的影响[J/OL].分子植物育种,(2021-06-11)[2022-03-20].http://kns.cnki.net/kcms/detail/46.1068.S.20210611.0949.002.html.[29]侯思璇,李传哲,刘国华,等.盐胁迫对毛竹实生苗形态生理生化特征的影响[J].江苏农业科学,2021,49(10):136-141.[30]严卓立,李爱,张源一,等.十二种薄荷对NaCl胁迫的生理响应与综合评价[J].植物生理学报,2021,57(1):159-168.
相似文献/References:
[1]傅巧娟,李春楠,陈一,等.我国一串红的研究进展[J].北方园艺,2013,37(04):192.
[2]金 兰,陈 志.青海肋果沙棘和中国沙棘种子萌发特性比较研究[J].北方园艺,2014,38(13):39.
JIN Lan,CHEN Zhi.Comparative Study on Germination Characteristics of Hippophae neurocarpa and Hippophae rhamnoides Seeds[J].Northern Horticulture,2014,38(04):39.
[3]石秋梅,邓翻云,吴敏言,等.罗布麻和大叶白麻种子萌发及幼苗生长耐盐性研究[J].北方园艺,2014,38(12):128.
SHI Qiu-mei,DENG Fan-yun,WU Min-yan,et al. Study on Salt Tolerance of Apocynum venetum Linn.and Poacynum hendersonii(Hook.f.) Woodson at Stages of Seed Germination and Seedlings Growth[J].Northern Horticulture,2014,38(04):128.
[4]梁 芳,衣采洁.不同浸种处理对小冠花及蛇鞭菊种子萌发的影响[J].北方园艺,2014,38(11):62.
LIANG Fang,YI Cai-jie.Infulence of Different Processing Methods on the Seed Germination of Coronilla varia and Liatris spieata [J].Northern Horticulture,2014,38(04):62.
[5]崔兴国.不同浓度碳酸氢钠胁迫对圆叶牵牛种子发芽的影响[J].北方园艺,2014,38(11):74.
CUI Xing-guo.Effect of Different Concentration of NaHCO3 Stress on Seed Germination of Pharbitis purpurea(L.)Voigt[J].Northern Horticulture,2014,38(04):74.
[6]常青山,张利霞,郑轶琦,等.盐胁迫下钙离子对苜蓿种子萌发的影响[J].北方园艺,2014,38(10):64.
CHANG Qing-shan,ZHANG Li-xia,ZHENG Yi-qi,et al.Effects of Ca2+?on Seed Germination Characteristics of Medicago sativa Under Salt Stress[J].Northern Horticulture,2014,38(04):64.
[7]张 娇,张大治,马 艳,等.氯化钠模拟盐胁迫对沙冬青种子萌发和幼苗生长的影响[J].北方园艺,2014,38(14):65.
ZHANG Jiao,ZHANG Da-zhi,MA Yan,et al.Effect of NaCl Simulation of Salt Stress on Seed Germination and Seedling Growth of Ammopiptanthus mongolicus[J].Northern Horticulture,2014,38(04):65.
[8]杨 飞,郭海波,吴 菊,等.NaCl胁迫对白菜种子萌发及幼苗生长的影响[J].北方园艺,2014,38(01):26.
YANG Fei,GUO Hai-bo,WU Ju,et al.Effect of NaCl Stress on the Seed Germination and Seedling Growth of Chinese Cabbage Cultivars[J].Northern Horticulture,2014,38(04):26.
[9]张雷,李永洁,王雅茹,等.海水胁迫对半枝莲种子萌发的影响[J].北方园艺,2013,37(13):69.
ZHANG Lei,LI Yong-jie,WANG Ya-ru,et al.Effect of Seawater Stress on Scutellaria barbata Seed Germination[J].Northern Horticulture,2013,37(04):69.
[10]何会流.外源水杨酸对盐胁迫下凤仙花种子萌发特性的影响[J].北方园艺,2013,37(13):75.
HE Hui-liu.Effect of Exogenous Salicylic Acid on the Seeds Germination Characters of Impatiens balsamina L.Under NaCl Stress[J].Northern Horticulture,2013,37(04):75.
[11]黎韵琪,李明,唐堃,等.穿心莲营养体的化感自毒作用研究[J].北方园艺,2014,38(21):157.
LI Yun-qi,LI Ming,TANG Kun,et al.Study on Autotoxicity of Andrographis paniculata(Burm.F.)Nees Vegetative[J].Northern Horticulture,2014,38(04):157.
备注/Memo
第一作者简介：刘叶（1996-），女，硕士研究生，研究方向为中药资源开发及品质评价。E-mail:m18797011789@163.com.责任作者：邵艳华（1987-），女，博士，讲师，现主要从事中药资源开发及品质评价等研究工作。E-mail：shaoyh_gz@126.com.基金项目：广东省重点领域研发计划“现代种业”重大专项资助项目（2020B20221002）。收稿日期：2022-04-22