GU Fengju,LAI Liangmin,DU Minru,et al.Effects of Space Mutagenesis on Biological Characteristics of Volvariella volvacea[J].Northern Horticulture,2022,(17):112-120.[doi:10.11937/bfyy.20220543]
航天诱变处理对草菇生物学特性的影响
- Title:
- Effects of Space Mutagenesis on Biological Characteristics of Volvariella volvacea
- 文献标志码:
- A
- 摘要:
- 以草菇“V106”为试材，采用稳定高效的航天诱变技术处理草菇菌株“V106”，研究了航天诱变对诱变株菌丝和子实体生长、胞内胞外活性物质含量、鲜菇贮藏特性等生物学特性的影响，以期为草菇的诱变育种提供新思路。结果表明：筛选得到7株诱变菌株，生物学特性发生了不同程度的改变。其中，所得草菇诱变株T7菌株丝生长速度提高了9.42%，出菇时间减少至13 d，鲜菇质量提高了1.37%，胞内麦角硫因、胞内外总酚以及胞外类黄酮含量均优于“V106”，保鲜期延长至4 d。菌株T7的成功筛选表明航天诱变可作为草菇选育的有效途径，促进草菇产业的积极发展。
- Abstract:
- Straw mushroom ‘V106’ was used as the test material and stable and efficient aerospace mutagenesis was applied to treat strain ‘V106’.The effects of aerospace mutagenesis on the biological characteristics of the mutant strain,including mycelial and substrate growth,intracellular and extracellular active substance content and storage characteristics of fresh mushrooms were studied,in order to provide new ideas for mutation breeding of Volvariella volvacea.The results showed that seven mutagenic strains were screened and their biological characteristics were changed to various degrees.Among them,the mycelial growth rate of mutant strain T7 was increased by 9.42%,the emergence time was reduced to 13 days,the mushroom quality was increased by 1.37%,the contents of intracellular ergothioin,total intracellular and extracellular phenols and extracellular flavonoids were better than those of ‘V106’,and the shelf life was extended to 4 days.The successful screening of strain T7 showed that space mutation can be used as an effective way for the breeding of Volvariella volvacea and promoted the positive development of Volvariella volvacea industry.
参考文献/References:
[1]DAS D,MONDAL S,ROY S K,et al.A (1→6)-β-glucan from a somatic hybrid of Pleurotus florida and Volvariella volvacea:Isolation,characterization,and study of immunoenhancing properties[J].Carbohydrate Research,2010,345(7):974-978.[2]SUN X,HUANG W,XIAO S,et al.Extracellular expression and efficient purification of a functional recombinant Volvariella volvacea immunomodulatory protein (FIP-vvo) using Pichia pastoris system[J].Protein Expression and Purification,2014,94:95-100.[3]MAITI S,BHUTIA S K,MALLICK S K,et al.Antiproliferative and immunostimulatory protein fraction from edible mushrooms[J].Environmental Toxicology and Pharmacology,2008,26(2):187-191.[4]WU J,CHEN C,CHANG W,et al.Anti-cancer effects of protein extracts from Calvatia lilacina,Pleurotus ostreatus and Volvariella volvacea[J].Evidence-based Complementary and Alternative Medicine,2011,2011(2):982368.[5]WU F,ZHOU L,YANG Z,et al.Resource diversity of Chinese macrofungi:Edible,medicinal and poisonous species[J].Fungal Diversity,2019,98(1):1-76.[6]CHEUNG L M,CHEUNG P,OOI V.Antioxidant activity and total phenolics of edible mushroom extracts[J].Food Chemistry,2003,81(2):249-255.[7]DAPENG B,MING G,HUAJUN Z,et al.Sequencing and comparative analysis of the straw mushroom (Volvariella volvacea) genome[J].PLoS One,2013,8(3):e58294.[8]刘昆昂,刘萌,张根伟,等.金针菇遗传育种研究进展[J].江苏农业科学,2019,47(14):18-22.[9]王振福,孟宪志,邓春海,等.食用菌空间诱变育种研究初报[J].空间科学学报,1996(S1):160-161.[10]侯文井,王露露,栗铭鸿,等.4种长白山区食用菌多酚类物质的体外抗氧化活性[J].食品工业,2017,38(1):18-22.[11]杨文慧,孟敏,王晓飞,等.不同食用菌的抗氧化活性及总黄酮含量测定研究[J].时珍国医国药,2020,31(2):300-303.[12]XIAO L,SUN S,LI K,et al.Effects of nanobubble water supplementation on biomass accumulation during mycelium cultivation of Cordyceps militaris and the antioxidant activities of extracted polysaccharides[J].Bioresource Technology Reports,2020,12(2):100600.[13]莫宇丽,李亚欢,王艳,等.响应面法优化超声微波联合提取杏鲍菇中麦角硫因工艺[J].食品工业科技,2020,41(1):143-149.[14]陈艳霞,秦超,张姗姗,等.银丝草菇子实体、菌丝体和发酵液中营养成分比较分析[J].中国食用菌,2021,40(3):71-78.[15]杨佳乐,范桂枝,张李香.秋水仙素诱变对桑黄菌丝生长及活性成分的影响[J].北方园艺,2021(7):110-116.[16]范秀芝,姚芬,王世斌,等.高产多糖和麦角甾醇姬松茸菌株的诱变选育[J].食用菌学报,2021,28(3):55-62.[17]武晓雨,邓百万,陈文强,等.紫外诱变选育耐高温香菇新品种[J].浙江农业学报,2017,29(12):2015-2022.[18]林锋.单孢杂交选育草菇耐低温高产新菌株[D].上海：上海海洋大学,2015.[19]何建华,蒋玮,吕贝贝,等.ARTP诱变筛选草菇优良菌株及RAPD分析[J].核农学报,2014,28(11):1950-1955.[20]赵妍,颜素雅,林锋,等.利用分子标记辅助育种技术选育低温高产草菇菌株[J].分子植物育种,2017,15(8):3153-3159.[21]余昌霞,李正鹏,查磊,等.不同光质对草菇菌丝生长及子实体性状的影响[J].食用菌学报,2021,28(3):72-77.[22]韩业君.草菇耐低温菌株的诱变选育与分子生物学鉴定[D].南京：南京农业大学,2004.[23]谢宝贵,罗联忠.草菇菌柄不同发育时期内源激素变化研究[C].武汉:第六届中韩菌物学研讨会,2004.[24]陈志宏,陶永新,陈炳智,等.与草菇菌柄伸长相关的bZIP转录因子基因克隆与表达分析[J].基因组学与应用生物学,2014,33(3):585-590.[25]王玮,林俊芳,聂斐,等.不同草菇栽培菌株的比较[J].食用菌学报,2012,19(2):59-62.[26]吴振强.适合杏鲍菇菌渣栽培的草菇品种筛选[J].中国食用菌,2021,40(9):22-26.
相似文献/References:
[1]侯祥保,魏 峰,孔繁成.草菇-双孢菇周年轮作高效栽培技术[J].北方园艺,2014,38(10):143.
HOU Xiang-bao,WEI Feng,KONG Fan-cheng.Highly Effective Cultivation Technology of Agaricus Bisporus and Straw Mushroom by Annual Rotation[J].Northern Horticulture,2014,38(17):143.
[2]刘主,蔡爱群,刘静华,等.基于ITS区序列的疑似野生“南华”草菇菌株的分子鉴定[J].北方园艺,2016,40(01):126.[doi:10.11937/bfyy.201601033]
LIU Zhu,CAI Aiqun,LIU Jinghua,et al.Molecular Identification of Suspected Wild ‘Nanhua’ Volvariella volvacea Strain Based on Internal Transcribed Spacer Sequence[J].Northern Horticulture,2016,40(17):126.[doi:10.11937/bfyy.201601033]
[3]金 卫 根.草菇菌丝体液体培养条件的优化研究[J].北方园艺,2011,35(07):0.[doi:10.11937/bfyy.201107055]
JIN Wei-gen.Optimization of Straw Mushroom Mycelium Culture Conditions[J].Northern Horticulture,2011,35(17):0.[doi:10.11937/bfyy.201107055]
[4]李 贺 年,王 桂 英,柴 建 明,等.草 菇 高 产 栽 培 技 术[J].北方园艺,2009,33(06):0.[doi:10.11937/bfyy.200906087]
LI He - nian,W ANG Gui- ying,CHAI Jian - ming,et al.Probe on High Production Cultivation T echnique of Volvariella voluacea[J].Northern Horticulture,2009,33(17):0.[doi:10.11937/bfyy.200906087]
[5]温 海 祥,肖 洪 东,黄 剑 波,等.草菇种内原生质体融合育种研究[J].北方园艺,2000,24(03):0.[doi:10.11937/bfyy.200003037]
[J].Northern Horticulture,2000,24(17):0.[doi:10.11937/bfyy.200003037]
[6]陆珠,李玉,姚允武.赞比亚引种草菇的发酵料栽培及其效益分析[J].北方园艺,2017,41(06):148.[doi:10.11937/bfyy.201706035]
LU Zhu,LI Yu,YAO Yunwu.Fermentation Materials Cultivation and Benefit Analysis of Introducing Volvariella volvacea in Zambia[J].Northern Horticulture,2017,41(17):148.[doi:10.11937/bfyy.201706035]
[7]孟 丽,张兰增,周和云,等.数字基因表达谱解析草菇子实体的生长发育[J].北方园艺,2018,42(08):1.[doi:10.11937/bfyy.20173248]
MENG Li,ZHANG Lanzeng,ZHOU Heyun,et al.Digital Gene Expression Profiling Reveals Volvariella volvacea Fruiting Body Development[J].Northern Horticulture,2018,42(17):1.[doi:10.11937/bfyy.20173248]
[8]王闯,曹娜,刁宁宁,等.日光温室番茄-草菇-小西瓜一年三茬高效栽培模式[J].北方园艺,2019,43(05):200.[doi:10.11937/bfyy.20181641]
[J].Northern Horticulture,2019,43(17):200.[doi:10.11937/bfyy.20181641]
[9]查磊,仝宗军,李正鹏,等.隧道发酵技术在草菇工厂化[J].北方园艺,2022,(22):116.[doi:10.11937/bfyy.20220609]
ZHA Lei,TONG Zongjun,LI Zhengpeng,et al.Application of Tunnel Fermentation Technology in the Preparation of Volvariella volvacea Industrialized Cultivation Materials[J].Northern Horticulture,2022,(17):116.[doi:10.11937/bfyy.20220609]
[10]郎红,赵云昊,姜明亮.航天诱变育种在园艺作物中的应用进展[J].北方园艺,2024,(14):135.[doi:10.11937/bfyy.20240216]
LANG Hong,ZHAO Yunhao,JIANG Mingliang.Research Progress on Space Mutation Breeding Application of Horticultural Crops[J].Northern Horticulture,2024,(17):135.[doi:10.11937/bfyy.20240216]
备注/Memo
第一作者简介：谷凤举（1993-），男，硕士研究生，研究方向为食用菌遗传育种与贮藏保鲜。E-mail：1425111675@qq.com.责任作者：王杰（1978-），女，博士，教授，博士生导师，现主要从事食药用真菌生物工程等研究工作。E-mail：wjcasey@scau.edu.cn.基金项目：广东省重点领域研发计划资助项目（2018B020206001）；广东省科技专项资金资助项目（200714156270599）。收稿日期：2022-02-16