[1]范小玉,赵跃锋.2,4-表油菜素内酯对低温胁迫下茄子幼苗生长及生理特性的影响[J].北方园艺,2021,(22):7-13.[doi:10.11937/bfyy.20212172]
　FAN Xiaoyu,ZHAO Yuefeng.Effects of 2，4-Epbrassinolipids on Growth and Physiological Characteristics of Eggplant Seedlings Under Low Temperature Stress[J].Northern Horticulture,2021,(22):7-13.[doi:10.11937/bfyy.20212172]
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2,4-表油菜素内酯对低温胁迫下茄子幼苗生长及生理特性的影响

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2021年22 页码: 7-13 栏目: 出版日期: 2021-11-30
Title:
Effects of 2，4-Epbrassinolipids on Growth and Physiological Characteristics of Eggplant Seedlings Under Low Temperature Stress
作者:
范小玉; 赵跃锋
（商丘市农林科学院，河南 商丘 476000）
Author(s):
FAN Xiaoyu; ZHAO Yuefeng
(Shangqiu College of Agricultural Science，Shangqiu，Henan 476000)
关键词:
2; 4-表油菜素内酯; 低温胁迫; 茄子幼苗
Keywords:
2; 4-epbrassinolipid; low temperature stress; eggplant seedling
DOI:
10.11937/bfyy.20212172
文献标志码:
A
摘要:
以“商茄2号”为试材，采用不同浓度（0、0.01、0.05、0.10、0.50、1.00 mg?L-1）的2,4-表油菜素内酯(EBR)溶液进行低温（15 ℃/5 ℃）处理的方法，研究了EBR对茄子幼苗冷害指数、丙二醛(MDA)含量、抗氧化酶活性及幼苗生长指标的影响，以期为EBR提高茄子耐冷性机制的研究提供参考依据。结果表明：适宜浓度的外源EBR能够有效缓解低温胁迫对茄子幼苗的损伤，并以0.10 mg?L-1 EBR处理效果最佳。其幼苗冷害指数相比低温对照显著降低，有效缓解了幼苗冷害症状；而幼苗株高、茎粗、鲜质量和干质量相比低温对照分别提高了33.35%、77.62%、55.40%和34.73%，增加了茄子幼苗的生物量，为构建壮苗打下基础；同时0.10 mg?L-1 EBR处理能够显著降低茄子幼苗叶片MDA 含量的积累，提高幼苗叶片超氧化物歧化酶（SOD）、过氧化物酶（POD）和过氧化氢酶（CAT）活性。综上所述，在该试验条件下，喷施0.10 mg?L-1 EBR溶液，能有效提高茄子幼苗的低温耐受性，有利于培育茄子壮苗。
Abstract:
Taking ‘Shangqie 2’ eggplant as the test materials，the different concentrations (0，0.01，0.05，0.10，0.50，1.00 mg?L-1) of 2,4-epbrassinolipid (EBR) was used under low temperature (15 ℃/5 ℃)，the effects of EBR on the chilling injury index，malondialdehyde content，antioxidant enzyme activity and seedling growth indexes were studied，in order to provide reference for the study of the mechanism of EBR improving cold tolerance of eggplant.The results showed that the appropriate concentration of exogenous EBR could effectively reduce the damage of eggplant seedlings under low temperature stress.Among them，the treatment of 0.10 mg?L-1 EBR showed the best effect.The chilling injury index of the seedlings was significantly reduced compared with the control under low temperature stress.The plant height，stem diameter，fresh weight and dry weight of seedlings significantly increased by 33.35%,77.62%,55.40%,34.73% respectively.At the same time，the treatment of 0.10 mg?L-1 EBR could significantly reduce the accumulation of MDA in seedling leaves，while it could significantly improve the activities of SOD，POD and CAT in the leaves of eggplant seedling.In conclusion，spraying of 0.10 mg?L-1 EBR could effectively increase the low temperature tolerance of eggplant seedlings，which was beneficial to the cultivation of strong eggplant seedlings.

参考文献/References:

[1]阎世江,张继宁,刘洁.不同来源茄子耐低温性的鉴定[J].河北科技师范学院学报,2013,27(1):9-12．[2]王红红,李凯荣,侯华伟.油菜素内酯提高植物抗逆性的研究进展[J].干旱地区农业研究,2005,23(3):214-219.[3]BAJGUZ A,HAYAT S.Effects of brassinosteroids on the plant responses to environmental stresses[J].Plant Physiology and Biochemistry,2009,47:1-8.[4]LIMON-PACHECO J,GONSEBATT M E.The role of antioxidants and antioxidant-related enzymes in protective responses to environmentally induced oxidative stress[J].Mutation Research,2009,674:137-147.[5]KRISHNA P.Brassinosteroid-mediated stress responses[J].Journal of Plant Growth Regulation,2003（22）:289-297.[6]丁丹阳,张璐翔,朱智威,等.叶面喷施2,4-表油菜素内酯对烟草抗旱性的影响[J].中国烟草科学,2018,39(4):50-57.[7]束红梅,郭书巧,沈新莲,等.油菜素内酯对NaCl胁迫下棉花幼苗生理特性的影响[J].江苏农业学报,2011,27(6):1198-1202.[8]陆晓民,杨威.油菜素内酯对氯化钠胁迫下黄瓜幼苗的缓解效应[J].应用生态学报,2013,24(5):1409-1414.[9]张林青.油菜素内酯对盐胁迫下番茄幼苗生理指标的影响[J].北方园艺,2013(1):1-3.[10]张永平,陈幼源,杨少军.高温胁迫下2,4-表油菜素内酯对甜瓜幼苗生理及光合特性的影响[J].植物生理学报,2012,48(7):683-688.[11]FARIDUDDIN Q,YUSUF M,CHALKOO S,et al.28-homobrassinolide improves growth and photosynthesis in Cucumis sativus L.through an enhanced antioxidant system in the presence of chilling stress[J].Photosynthetica,2011,49(1):55-64.[12]张琳,唐敏,王正,等.油菜素内酯对油菜中有机磷农药毒死蜱残留的降解效果[J].安徽农业科学,2012,40(2):805-806.[13]张小贝,祝志欣,南文卓,等.2,4-表油菜素内酯(EBR)对菜用甘薯抗寒生理生化的影响[J].安徽农业大学学报,2017,44(3):525-529.[14]杨萍,李杰.2,4-表油菜素内酯对低温胁迫下辣椒幼苗抗氧化系统的影响[J].北方园艺,2017(21):7-12.[15]徐晓昀,郁继华,颉建明,等.2,4-表油菜素内酯对亚适温弱光下黄瓜幼苗光合特性和抗氧化系统的影响[J].核农学报,2017,31(5):979-986.[16]吴雪霞,杨晓春,查丁石,等.茄子幼苗耐低温性生理机制的研究[J].上海农业学报,2013,29(5):45-49.[17]李合生.植物生理学实验技术指导[M].北京:高等教育出版社,2001.[18]王永健,姜亦巍,曹宛虹,等.低温对不同品种黄瓜种子萌发、过氧化物酶及同功酶的影响[J].华北农学报,1995,10(2):72-76．[19]李光庆,谢祝捷,姚雪琴,等.花椰菜叶绿素荧光参数与耐寒性的关系研究[J].园艺学报,2010,37(12):2001-2006.[20]袁凌云,朱世东,赵冠艳,等.油菜素内酯诱导番茄幼苗抗冷效果的研究[J].中国农学通报,2010,26(5):205-208.[21]范小玉,张显.油菜素内酯对低温弱光胁迫下西瓜幼苗耐冷性的影响[J].北方园艺,2012(7):5-8.[22]杜秀敏,殷文璇,赵彦修,等.植物中活性氧的产生及清除机制[J].生物工程学报,2001,17(2):121-125.[23]杨莲,高欢,吴凤芝.2,4-表油菜素内酯对亚低温胁迫下番茄幼苗生长与钾积累的影响[J].中国蔬菜,2021(1):48-55.[24]LIANG Y C,CHEN Y C,CHEN Q,et al.Exogenous silicon(Si) increases antioxidant enzyme acvtivity and reduce lipid peroxidation in roots of salt-stressed barley(Hordeum vulgare L.)[J].Journal of Plant Physiology,2003,160(10):1157-1164.[25]HU W H,WU Y,ZENG J Z,et al.Chill-induced inhibition of photosynthesis was alleviated by 2，4-epibrassinolide pretreatment in cucumber during chilling and subsequent recovery[J].Photosynthetica,2010,48(4):537-544.[26]POLLE A.Dissecting the superoxide dismutase-ascorbate-glutathione-pathway in chloroplasts by metabolic modeling.Computer simulations as a step towards flux analysis[J].Plant Physiology,2001,126(1):445-462.[27]孙三杰,李建明,宗建伟,等.亚低温与干旱胁迫对番茄幼苗根系形态及叶片结构的影响[J].应用生态学报,2012,23(11):3027-3032.[28]周伟江,吴旺嫔,唐才宝,等.外源油菜素内酯对低温胁迫下水稻幼苗生长及生理特性的影响[J].西北农业学报,2020,29(9):1410-1416.[29]闫慧萍,彭云玲,赵小强,等.外源2,4-表油菜素内酯对逆境胁迫下玉米种子萌发和幼苗生长的影响[J].核农学报,2016,30(5):988-996.[30]吴杨,高慧纯,张必弦,等.2，4-表油菜素内酯对盐碱胁迫下大豆生育、生理及细胞超微结构的影响[J].中国农业科学,2017,50(5):811-821.[31]黄玉辉,黄如葵,陈小凤,等.油菜素内酯对苦瓜抗冷性生理指标的影响[J].南方农业学报,2012,43(5):592-596.[32]惠竹梅,王智真,胡勇,等.2,4-表油菜素内酯对低温胁迫下葡萄幼苗抗氧化系统及渗透调节物质的影响[J].中国农业科学,2013,46(5):1005-1013.

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备注/Memo

第一作者简介：范小玉（1984-），女，硕士，助理研究员，现主要从事茄子育种及栽培等研究工作。E-mail：fxy084627@126.com.责任作者：赵跃锋（1969-），男，本科，副研究员，现主要从事茄子育种及栽培等研究工作。E-mail：zyf9698@163.com.基金项目：商丘市农林科学院科技攻关资助项目（20190211）。收稿日期：2021-05-21

更新日期/Last Update: 2022-02-10