LIU Songhu,SUN Haoshuang,ZHANG Yuxue,et al.Effects of Exogenous EBR on Watermelon (Citrullus vulgaris Schrad.) Seed Germination and Seedling Growth Under NaCl Stress[J].Northern Horticulture,2024,(10):39-47.[doi:10.11937/bfyy.20233808]
外源EBR对NaCl胁迫下西瓜种子萌发及幼苗生长的影响
- Title:
- Effects of Exogenous EBR on Watermelon (Citrullus vulgaris Schrad.) Seed Germination and Seedling Growth Under NaCl Stress
- 文章编号:
- 1001-0009(2024)10-0039-09
- Keywords:
- Citrullus vulgaris Schrad.; salt stress; 2; 4-epibrassinolide; phenotypic traits; physiological characteristics
- 分类号:
- S 651
- 文献标志码:
- A
- 摘要:
- 以西瓜优良品种“早佳8424”为试材，用不同浓度的EBR对NaCl胁迫下的西瓜种子进行浸种处理和西瓜幼苗喷施处理，研究在NaCl胁迫下不同浓度2,4-表油菜素内酯（EBR）对西瓜种子萌发、幼苗生长和生理特性的影响，以期筛选出适宜的EBR浓度应用于生产实践。结果表明：在150 mmol·L-1 NaCl胁迫下，适宜浓度的EBR对西瓜种子的发芽势、发芽率具有显著缓解作用，0.05 mg·L-1 EBR处理显著提高盐胁迫下西瓜幼芽生长后的下胚轴长、主根长及鲜质量。在盐胁迫下，西瓜叶面喷施0.05 mg·L-1EBR能显著促进幼苗的根长、株高、叶长与叶宽的增长；EBR处理后的西瓜幼苗丙二醛（MDA）含量与只施加150 mmol·L-1 NaCl的处理相比显著降低，西瓜幼苗根系活力、超氧化物歧化酶（SOD）活性和过氧化物酶（POD）活性、脯氨酸含量在0.05 mg·L-1 EBR浓度处理下显著高于其他EBR浓度处理。综上所述，0.05 mg·L-1 EBR处理能显著促进盐胁迫下西瓜种子萌发和幼苗的生长，可为西瓜生产遇到的盐胁迫问题提供参考依据。
- Abstract:
- Taking the excellent watermelon variety ‘Zaojia 8424’ as the test material,different concentrations of EBR were used to soak and spray watermelon seeds under NaCl stress.The effects of different concentrations of 2,4-epibrassinolide (EBR) on watermelon seed germination,seedling growth,and physiological characteristics under NaCl stress were studied,in order to screen suitable EBR concentrations for production practice.The results showed that under the stress of 150 mmol·L-1 NaCl,the appropriate concentration of EBR significantly alleviated the germination potential and germination rate of watermelon seeds,and 0.05 mg·L-1 EBR treatment significantly increased the hypocotyl length,main root length and fresh weight of watermelon seedlings after growth.Under salt stress,spraying 0.05 mg·L-1 EBR on watermelon leaf surface could significantly promote the growth of root length,plant height,leaf length and leaf width of seedlings.The MDA content of watermelon seedlings treated with EBR was significantly lower than that treated with 150 mmol·L-1 NaCl.The root activity,SOD and POD activities and proline contents of watermelon seedlings treated with 0.05 mg·L-1 EBR were significantly higher than those treated with other EBR concentrations.In conclusion,0.05 mg·L-1 EBR treatment can significantly promote the seed germination and seedling growth of watermelon under salt stress,which can provide reference for salt stress problems in watermelon production.
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备注/Memo
第一作者简介：刘松虎（1977-），男，硕士，副教授，现主要从事蔬菜栽培生理与种质创新等研究工作。E-mail：shliu2012@126.com.责任作者：朱庆松（1976-），男，硕士，副教授，现主要从事设施作物栽培与遗传改良等研究工作。E-mail：357734686@qq.com.基金项目：河南省科技攻关资助项目（222102110443）；河南省高等学校重点科研资助项目（21B210009）。收稿日期：2023-12-08