LI Xiang,XIONG Yanan,JIN Yazhong,et al.Effects of ETH,ABA and EtOH on Softening and Regulatory Enzyme Activities in Postharvest Oriental Melon[J].Northern Horticulture,2021,(21):100-108.[doi:10.11937/bfyy.20211333]
乙烯、脱落酸以及乙醇对采后薄皮甜瓜果实软化及调节酶活性的影响
- Title:
- Effects of ETH,ABA and EtOH on Softening and Regulatory Enzyme Activities in Postharvest Oriental Melon
- Keywords:
- oriental melon; fruit softening; ethylene; ethanol; ABA; enzyme activity
- 文献标志码:
- A
- 摘要:
- 以薄皮甜瓜果实为试材,用外源乙烯、脱落酸以及乙醇对采后薄皮甜瓜果实进行处理后于常温(23±1)℃条件下贮藏,研究贮藏期间各组薄皮甜瓜果实硬度及相关酶活性变化情况,以期为薄皮甜瓜采后果实品质的维持及保鲜技术的完善与应用提供参考依据。结果表明:贮藏期间,薄皮甜瓜果实硬度呈降低趋势;脱落酸(ABA)处理的果实硬度与果实中聚半乳糖醛酸酶(PG)、果胶酯酶(PME)、β-半乳糖苷酶(β-Gal)、β-D-葡聚糖酶(EGase)、淀粉酶活性升高相关,且ABA主要促进了贮藏前期果实中EGase以及淀粉酶活性,加速了原果胶、淀粉的分解,促进了贮藏前期果实的软化;而乙烯(ETH)处理果实硬度的降低与果实中PG、β-Gal活性明显呈负相关,且ABA和ETH处理均能通过提高果实贮藏前期LOX活性和电导率影响了果实硬度,可能存在羟自由基氧化作用对果实硬度的影响。在贮藏后期,ABA和ETH处理对果实软化的作用相似。乙醇(EtOH)则通过抑制PG、PME、β-Gal、EGase、淀粉酶以及LOX活性,延缓了果实可溶性果胶和可溶性糖以及电导率的升高,维持了薄皮甜瓜果实硬度。综上可知,ABA和ETH调控薄皮甜瓜果实软化的酶调系统存在差异,而EtOH能通过降低果胶及淀粉代谢酶活性抑制羟自由基的作用,进而减缓了果实软化进程,维持了果实硬度。
- Abstract:
- The oriental melon fruits were used as experimental materials,and stored at room temperature (23±1)℃ after treated with ethylene (ETH),abscisic acid (ABA) and ethanol (EtOH),the changes of the firmness and the enzyme activity of oriental melon fruits during storage were studied,in order to provide theoretical basis for maintaining the quality of oriental melon fruits,perfect and apply the technique of fresh-keeping technology.The results showed that the fruit firmness of oriental melon showed decreasing trend during storage.Treatments with ABA significantly affected the fruit firmness,which seemed to be related to the activities of PG,PME,β-gal,EGase and amylase.It mainly promoted the activities of EGase and amylase in fruits during the early storage period,accelerated the decomposition of pectin and starch,and promoted the softening of the fruits during the early storage period.However,treatment with ETH decreased the fruit firmness,which were negatively related with the activities of PG and β-Gal in fruits,ABA and ETH treatments could affect fruit firmness by increasing LOX activity and electrical conductivity in the early stage of fruit storage,and there might be an effect of hydroxyl radical oxidation on fruit firmness.In the late storage period,ABA and ETH treatments had similar effects on fruit softening.Treatment with EtOH delayed the increasing of soluble pectin,soluble sugar and electrical conductivity of the fruits,and maintains the firmness of the oriental melon fruit by inhibits the activities of PG,PME,β-Gal,EGase,amylase and LOX.In conclusion,there were differences in the enzyme regulation systems of ABA and ETH regulating the softening of melon fruit,whereas EtOH could inhibit the effect of hydroxyl free radicals by reducing the activity of pectin and starch metabolism enzymes,thereby slowed the fruits softening process and maintaining the fruit firmness.
参考文献/References:
[1]JIN Y Z,LYU D Q,LIU W W,et al.Ethanol vapor treatment maintains postharvest storage quality and inhibits internal ethylene biosynthesis during storage of oriental sweet melons[J].Postharvest Biology and Technology,2013,86:372-380.[2]POS S,PANIAGUA C,MATAS A,et al.A nanostructural view of the cell wall disassembly process during fruit ripening and postharvest storage by atomic force microscopy[J].Trends in Food Science and Technology,2019,87:47-58.[3]WANG D,YEATS T H,ULUISIK S,et al.Fruit softening:Revisiting the role of pectin[J].Trends in Plant Science,2018,23(4):302-310.[4]HIRSCH M,LANGER S E,MARINA M,et al.Expression profiling of endo-xylanases during ripening of strawberry cultivars with contrasting softening rates.Influence of postharvest and hormonal treatments[J].Journal of the Science of Food and Agriculture,2020,101(9):3676-3684.[5]AIRIANAH O B,VREEBURG R A,FRY S C.Pectic polysaccharides are attackedby hydroxyl radicals in ripening fruit:Evidence from a fluorescent fingerprinting method[J].Annals of Botany,2016,117(3):441-455.[6]魏建梅,齐秀东,张海娥,等.京白梨果实采后PG、糖苷酶和LOX活性变化及其基因表达特性[J].园艺学报,2012,39(1):31-39.[7]YUAN L,BI Y,GE Y H,et al.Postharvest hot water dipping reduces decay by inducing diseaseresistance and maintaining firmness in muskmelon (Cucumis melo L.)fruit[J].Scientia Horticulturae,2013,161:101-110.[8]张敏,王爱玲,杨军,等.甜瓜‘黄醉仙’果实采后软化过程中细胞壁水解酶的变化[J].西北农林科技大学学报,2015,43(4):113-117.[9]再吐娜·买买提,张小梅,马西欣,等.不同保鲜处理对甜瓜采后贮藏性状的影响[J].中国农学通报,2017,33(19):52-56.[10]张强,代文婷,金新文.氯化钙与1-甲基环丙烯对伽师瓜果实软化与果胶酶活性及其基因表达影响[J].食品与发酵工业,2018,45(2):45-49.[11]朱婉彤,冯作山,白羽嘉,等.乙烯和1-MCP 对伯谢克辛甜瓜果实软化及细胞壁降解的影响[J].现代食品科技,2019,35(12):94-101.[12]LI Y,QI H Y,JIN Y Z,et al.Role of ethylene in the biosynthetic pathway of related-aroma volatiles derived from fatty acids in oriental sweet melon[J].Scientia Horticulturae,2016,141(4):327-338.[13]吕双双,李天来,吴志刚,等.外源乙烯对不同成熟度网纹甜瓜果实细胞壁水解酶活性的影响[J].西北农业学报,2009,18(4):344-350.[14]刘廷旭.ABA对桃果实成熟软化和乙烯释放信号转导组分的影响[D].杨凌:西北农林科技大学,2012.[15]陈昆松,李方,张上隆.ABA和IAA对猕猴桃果实成熟进程的调控[J].园艺学报,1999(2):13-18.[16]范婷婷.ABA和乙烯对树莓果实成熟软化的影响[D].哈尔滨:东北农业大学,2017.[17]PANIAGUA C,SANTIAGO-DOMNECH N,KIRBY A R,et al.Structural changes in cell wall pectins during strawberry fruit development[J].Plant Physiology and Biochemistry,2017,118:55-63.[18]曹建康,姜微波.果蔬采后生理生化实验指导[M].北京:中国轻工业出版社,2007.[19]REN Y Y,SUN P P,WANG X X,et al.Degradation of cell wall polysaccharides and change of related enzyme activities with fruit softening in Annona squamosa during storage[J].Postharvest Biology and Technology,2020,166:1-8.[20]ZHAO S,WEN J,WANG H,et al.Changes in lignin content and activity of related enzymes in the endocarp during the walnut shell development period[J].Horticultural Plant Journal,2016,2(3):141-146.[21]HUANG L N,WU G B,ZHANG S,et al.The identification and functional verification of the cinnamate 4-hydroxylase gene from wax apple fruit and its role in lignin biosynthesis during nitric oxide-delayed postharvest cottony softening[J].Postharvest Biology and Technology,2019,158:1-9.[22]DING Q Q,YANG X X,PI Y,et al.Genome-wide identification and expression analysis of extensin genes in tomato[J].Genomics,2020,112(6):4348-4360.[23]冯新,赖瑞联,高敏霞,等.Adβgal-1和Adβgal-2克隆及其在猕猴桃果实软化中的作用[J].中国农业科学,2019,52(2):312-326.[24]XUE C,GUAN S C,CHEN J Q,et al.Genome wide identification and functional characterization of strawberry pectin methylesterases related to fruit softening[J].BMC Plant Biology,2020,20(1):13-30.[26]王琛,高雅,陶烨,等.60Co-γ 辐照对冷藏蓝莓果实软化相关指标的影响[J].食品安全质量检测学报,2020,11(22):8540-8546.[27]董晓庆,刘洪滩,朱守亮,等.1-MCP 结合自发气调包装对‘空心李’果实软化和细胞壁代谢的影响[J].中国农学通报,2020,36(22):129-135.[28]何俊瑜,任艳芳,陈元有,等.一氧化氮对常温贮藏下芒果果实软化和细胞壁代谢的影响[J].食品工业科技,2018,39(17):269-275.[29]MCATEE P,KARIM S,SCHAFFER R,et al.A dynamic interplay between phytohormones is required for fruit development,maturation,and ripening[J].Fronterin Plant Science,2013(4):1-7.[30]KUMAR R,KHURANA A,SHARMA A K.Role of plant hormones and their interplay in development and ripening of fleshy fruits[J].Journal of Experimental Botany,2014,65(16):4561-4575.[31]ZHANG M,YUAN B,LENG P.The role of ABA in triggering ethylene biosynthesis and ripening of tomato fruit[J].Journal of Experimental Botany,2009,60(6):1579-1588.[32]刘廷旭,罗川,赵彩平,等.脱落酸对桃果实成熟软化和乙烯生物合成的影响[J].北方园艺,2012(2):134-137.[33]LI X W,CAO S F,ZHENG Y H,et al.1-MCP suppresses ethylene biosynthesis and delays softening of ‘Hami’ melon during storage at ambient temperature[J].Journal of the Science of Food and Agriculture,2011,91(14):2684-2688.[34]LINDO-GARCA V,MUOZ P,LARRIGAUDIRE C,et al.Interplay between hormones and assimilates during pear development and ripening and its relationship with the fruit postharvest behavior[J].Plant Science,2020,291:1-10.[35]高维亚,吴光斌,陈发河.NO处理对采后莲雾果实絮状绵软进程的影响[J].食品科学,2016,37(18):208-215.[36]PANIAGUA C,RIC-VARAS P,GARCA-GAGO J A,et al.Elucidating the role of polygalacturonase genes in strawberry fruit softening[J].Journal of Experimental Biology,2020,71(22):7103-7117.[37]王刚,贾展慧,张计育,等.‘槜李’果实软化相关基因PsPG的克隆与表达分析[J].植物资源与环境学报,2017,26(4):60-66.[38]JIN Y Z,ZHANG C,LIU W,et al.The alcohol dehydrogenase gene family in melon (Cucumis melo L.):Bioinformatic analysis and expression patterns[J].Frontiers in Plant Science,2016(7):670-688.
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备注/Memo
第一作者简介:李响(1986-),男,硕士,助理研究员,现主要从事设施园艺与配套栽培技术、果实品质调控等研究工作。E-mail:6880506@163.com.责任作者:靳亚忠(1975-),男,博士,副教授,硕士生导师,现主要从事设施蔬菜栽培生理学、采后果蔬品质分子生物学以及木霉菌开发与应用等研究工作。E-mail:jyz_hsp@126.com.基金项目:校级重点培育课题资助项目(XA2016-01);现代农业产业技术体系国家西甜瓜产业技术体系资助项目(CARS-25);博士科研启动基金资助项目(XDB2015-01)。收稿日期:2021-03-28