WANG Peng,GUO Li,LIU Shuang,et al.Synergistic Effect on Antioxidant Activity of Chlorogenic Acid Extract[J].Northern Horticulture,2021,(15):106-111.[doi:10.11937/bfyy.20205387]
绿原酸提取物的抗氧化活性协同增效作用
- Title:
- Synergistic Effect on Antioxidant Activity of Chlorogenic Acid Extract
- 文献标志码:
- A
- 摘要:
- 以蒲公英和葵花籽粕绿原酸提取物为试材，采用FRAP亚铁离子还原能力为考察指标，研究了蒲公英和葵花籽粕来源的绿原酸提取物对抗氧化活性的影响因素，以抗氧化活性的半抑制浓度IC50为复配依据，评价蒲公英绿原酸与葵花籽粕复配品的抗氧化协同增效作用,以期为开发绿色高效的天然抗氧化剂提供参考依据。结果表明：反应体系在pH为4.0、37 ℃条件下，蒲公英和葵花籽粕绿原酸对FRAP亚铁离子还原能力达到最高，分别为1.11、1.09。蒲公英绿原酸可提高葵花籽粕复配品对FRAP亚铁离子的还原能力，具有抗氧化协同增效作用。
- Abstract:
- The chlorogenic acid extract of dandelion and sunflower seed meal were selected as test materials, and antioxidant activities of chlorogenic acid form dandelion and sunflower seed meal were determined using FRAP ferrous ion reducing capacity.The synergistic antioxidant activities of chlorogenic acid from dandelion and sunflower seed meal were evaluated on IC50 values for developing green and efficient natural antioxidants.The results showed that FRAP ferrous ion reducing capacity of chlorogenic acid from dandelion and sunflower seed meal was high level of 1.11 and 1.09 at pH 4.0 and 37 ℃.The compound of chlorogenic acid from dandelion and sunflower seed meal increased in FRAP ferrous ion reducing capacity with synergistic antioxidant effect.
参考文献/References:
[1]李玉山.绿原酸在天然植物中的分布和提取纯化工艺研究进展[J].解放军药学学报,2012,28(4):355-359.[2]宰学明,吴国荣,龚祝南,等.旱生和湿生生境对蒲公英体内抗氧化物质的影响[J].植物研究,2002,22(2):196-200.[3]赵涛.葵花籽粕中绿原酸和蛋白酶解肽的制备及生物活性研究[D].呼和浩特:内蒙古农业大学,2013.[4]李立顺,时维静,方婷,等.多种提取方法比较野生与栽培蒲公英绿原酸含量[J].中兽医医药杂志,2007(6):24-26.[5]宁伟,贾庆飞,朱丹,等.东北地区11种蒲公英咖啡酸和绿原酸的含量测定[J].沈阳农业大学学报,2012,43(5):595-598.[6]VASILIKI K,NIKOLAOS K,MARIA A,et al.Integrated sunflower-based biorefinery for the production of antioxidants,protein isolate and poly (3-hydroxybutyrate)[J].Industrial Crops and Products,2015,71(9):106-113.[7]YOSHIAKI A,MORIO Y,SAORI Y,et al.Isolation of phenolic constituents and characterization of antioxidant markers from sunflower (Helianthus annuus) seed extract[J].Phytochemistry Letters,2013,6(2):302-305.[8]ASTAFIEVA A A,ROGOZHIN E A,ODINTSOVA T I,et al.Discovery of novel antimicrobial peptides with unusual cysteine motifs in dandelion Taraxacum officinale Wigg[J].Flowers Peptides,2012,36(2):266-271.[9]XUEY S,ZHANG S M,DU M,et al.Dandelion extract suppresses reactive oxidative species and inflammasome in intestinal epithelial cells[J].Journal of Functional Foods,2017,29:10-18.[10]CHO S Y,PARK J Y,PARK E M,et al.Alternation of hepatic antioxidant enzyme activities and lipid profile in streptozotocin-induced diabetic rats by supplementation of dandelion water extract[J].Clinica Chimica Acta,2002,317(1-2):109-117.[11]MUNKHTUGS D,HAENG J H,HYE J Y,et al.Taraxacum official (dandelion) leaf extract alleviates high-fat diet-induced nonalcoholic fatty liver original research article[J].Food and Chemical Toxicology,2013,58(8):30-36.[12]ZHU H H,ZHAO H Y,ZHANG L J,et al.Dandelion root extract suppressed gastric cancer cells proliferation and migration through targeting lncRNA-CCAT1[J].Biomedicine & Pharmacotherapy,2017,93:1010-1017.[13]KATRIN M,HARALD S,JENNIFER F,et al.Dandelion (Taraxacum officinale) extracts supply antitumour effects of mistletoe in pediatric cancer cells[J].Phytomedicine,2015,22:25-26.[14]CHIEN J T,CHANG R H,HSIEH C H,et al.Antioxidant property of Taraxacum formosanum Kitam and its antitumor activity in non-small-cell lung cancer cells[J].Phytomedicine,2018,491(10):1-10.[15]DARIUSZ J,BOGDAN K,BERNADETTA L,et al.Evaluation of antioxidant activity of phenolic fractions from the leaves and petals of dandelion in human plasma treated with H2O2 and H2O2 /Fe[J].Chemico-Biological Interactions,2017,262:29-37.[16]XIE P J,HUANG L X,ZHANG C H,et al.Skin-care effects of dandelion leaf extract and stem extract:Antioxidant properties,tyrosinase inhibitory and molecular docking simulations[J].Industrial Crops and Products,2018,111(1):238-246.[17]中华人民共和国国家卫生和计划生育委员会.GB/T 5009.6-2016食品安全标准 食品中脂肪的测定[S].北京:中国标准出版社,2016.[18]HU Q P,XU J G.Profiles of carotenoids,anthocyanins,phenolics,and antioxidant activity of selected color waxy corn grains during maturation[J].Journal of Agricultural and Food Chemistry,2011,59(5):2026-2033.[19]杨剀舟.绿原酸抗氧化活性及其自协同抗氧化机理研究[D].北京:中国农业大学,2016.[20]陆丽琴.栀子不同采收期及干燥技术对绿原酸含量影响的研究[D].福州:福建农林大学,2012.
相似文献/References:
[1]李 光,余 霜.应用正交设计优化甘薯叶绿原酸提取工艺研究[J].北方园艺,2014,38(11):119.
LI Guang,YU Shuang.Study on Extraction Technology for Chlorogenic Acid From Sweet Potato Leaves by Orthogonal Design[J].Northern Horticulture,2014,38(15):119.
[2]赵权.六个山葡萄品种葡萄籽中多酚含量比较研究[J].北方园艺,2013,37(01):15.
ZHAO Quan.The Comparative Study on Polyphenol Contents in Seeds of Six Vitis amurensis Varieties[J].Northern Horticulture,2013,37(15):15.
[3]刘丽娟,徐鸿雁,王宏伟.蒲公英有效成分抑菌试验研究[J].北方园艺,2013,37(01):180.
LIU Li-juan,XU Hong-yan,WANG Hong-wei.Study on Effective Antimicrobial Ingredients Experimental of Taraxacum mongolicum Hand.-Mazz[J].Northern Horticulture,2013,37(15):180.
[4]刘灵娣,高雪飞,温春秀,等.“河北香菊”营养器官中活性成分积累规律研究[J].北方园艺,2013,37(19):162.
LIU Ling-di,GAO Xue-fei,WEN Chun-xiu,et al.Study on the Active Element Accumulation of Vegetative Organs in‘Hebei Xiangju’[J].Northern Horticulture,2013,37(15):162.
[5]刘灵娣,谢晓亮,高雪飞,等.河北香菊绿原酸提取工艺研究[J].北方园艺,2012,36(21):152.
LIU Ling-di,XIE Xiao-liang,GAO Xue-fei,et al.Study on the Extraction Technology of Chlorogenic Acid in ‘Hebeixiangju’[J].Northern Horticulture,2012,36(15):152.
[6]陶宏征,田学军,夏芳,等.三叶鬼针草水提液对蒲公英种子萌发和幼苗生长的化感作用[J].北方园艺,2015,39(10):87.[doi:10.11937/bfyy.201510021]
TAO Hong-zheng,TIAN Xue-jun,XIA Fang,et al.Allelopathic Effect of Bidens pilosa Extracts on Germination and Seedling Growth of Taraxacum mongolicum[J].Northern Horticulture,2015,39(15):87.[doi:10.11937/bfyy.201510021]
[7]王 鹏,郭 丽.响应面法优选蒲公英中绿原酸的提取工艺研究[J].北方园艺,2010,34(23):0.[doi:10.11937/bfyy.201023016]
W ANG Peng,GUO Li.Optimization of Chlorogenic Acid Extracting of Dandelion by Response Surface Methodology[J].Northern Horticulture,2010,34(15):0.[doi:10.11937/bfyy.201023016]
[8]关颖丽,蔡黎明,杨荣艳.北五加皮不同时间水提物中绿原酸含量的变化研究[J].北方园艺,2011,35(10):0.[doi:10.11937/bfyy.2011010048]
GUAN Ying-li,CAI Li-ming,YANG Rong-yan.Study on the Content Change of the Chlorogenic Acid in Water Extract of Cortex periplocae at the Different Times[J].Northern Horticulture,2011,35(15):0.[doi:10.11937/bfyy.2011010048]
[9]赵 磊,杨 延 杰,林 多.蒲 公 英 光 合 特 性 的 研 究[J].北方园艺,2008,32(04):0.[doi:10.11937/bfyy.200804010]
ZHAO Lei,YANG Yan -jie,LIN Duo.Studies on the Photosynthetic Characteristics of Taraxacum Mongolicum[J].Northern Horticulture,2008,32(15):0.[doi:10.11937/bfyy.200804010]
[10]韩文清,王建国,何媛媛,等.银翘复方药渣发酵处理试栽毛头鬼伞及主要药物成分分析[J].北方园艺,2017,41(02):145.[doi:10.11937/bfyy.201702035]
HAN Wenqing,WANG Jianguo,HE Yuanyuan,et al.Cultivation of Coprinus comatus Using the Fermented Chinese Medicine Residue From Yinqiao Detoxication Pills Production Waste and Analysis of the Active Pharmaceutical Ingredient[J].Northern Horticulture,2017,41(15):145.[doi:10.11937/bfyy.201702035]
备注/Memo
第一作者简介：王鹏（1981-），男，硕士，教授，现主要从事食品加工技术及食品工程等研究工作。E-mail:ljdrq@sina.com.基金项目：黑龙江省教育厅科研资助项目（2017-KYYWF-019）；黑龙江省省属高等学校基本科研业务费基础研究资助项目（KYYWF10236180301）；绥化学院绿色食品加工保鲜技术研究创新团队建设资助项目（SIT04B008）。收稿日期：2020-12-16