LI Yang,NIU Yonghua,ZHAO Lu.Function of Biological Crust in Soil Remediation in Fenhe River Basin[J].Northern Horticulture,2021,(14):100-106.[doi:10.11937/bfyy.20204176]
汾河流域修复中土壤生物结皮的功能研究
- Title:
- Function of Biological Crust in Soil Remediation in Fenhe River Basin
- Keywords:
- biological soil crust; physical and chemical properties of soil; soil enzyme activity; DGGE; dominant genus
- 文献标志码:
- A
- 摘要:
- 以汾河上游混交林系统为研究对象,测定了不同林地有无结皮条件下的土壤理化性质和5种酶活性,采用DGGE试验研究了藓结皮对土壤真菌和细菌的影响,测序比对确定了真菌细菌中的优势菌属,以期掌握生物土壤结皮对汾河上游土壤恢复的作用。结果表明:藓结皮提高了土壤的田间持水量、有机碳、全氮及全磷的含量和土壤蔗糖酶、脲酶、过氧化氢酶、多酚氧化酶和碱性磷酸酶的活性,同时增加了土壤真菌细菌的复杂性,测序比对表明根瘤菌属、硝化螺旋菌属和酸杆菌属是细菌的优势菌属,丝盖伞属和锥毛壳属是真菌的优势菌属。生物土壤结皮在汾河土壤恢复中起到了重要的作用。
- Abstract:
- Taking the regional mixed forest system in the upper reaches of Fenhe River as the research object,the soil physical and chemical properties and five kinds of enzyme activities with or without crusts under different forest conditions were determined.The effects of moss crusts on soil fungi and bacteria were studied by DGGE experiment,and the dominant bacteria were determined by sequencing.The results showed that moss crusts increased the soil water capacity,organic carbon,total nitrogen and total phosphorus contents,and the activities of sucrase,urease,catalase,polyphenol oxidase and alkaline phosphatase,and increased the complexity of soil fungal bacteria Spirochetes and acidobacteria are the dominant genera of bacteria,while fimbria and Chaetomium are the dominant genera of fungi.Biological soil crust plays an important role in soil restoration of Fenhe River.
参考文献/References:
[1]费燕明.汾河流域土壤侵蚀动态变化分析[D].太原:太原理工大学,2012.[2]李新荣,张元明,赵允格.生物土壤结皮研究:进展、前沿与展望[J].地球科学进展,2009,24(1):11-24.[3]迈克·费斯特.土壤生物结皮在干旱半干旱地区退化生态系统恢复中的作用[J].中国水土保持科学,2005,3(4):42-47.[4]FISCHER T,VESTE M,WIEHE W,et al.Water repellency and pore clogging at early successional stages of microbiotic crusts on inland dunes,Brandenburg,NE Germany[J].Catena,2010,80(1):47-52.[5]张培培.黄土丘陵区生物结皮对水分入渗的影响及模拟[D].北京:中国科学院研究生院,2014.[6]郭成久,陈乐,肖波,等.黄土高原苔藓结皮斥水性及其对火烧时间的响应[J].沈阳农业大学学报,2016(2):212-217.[7]赵裕栋,周俊,何璟.土壤微生物总DNA提取方法的优化[J].微生物学报,2012,52(9):1143-1150.[8]栗若兰,郑智慧,张华,等.从土壤中快速提取纯化DNA方法的建立[J].河北师范大学学报(自然科学版),2008,32(1):98-100,122.[9]MUYZER G,de WAAL E C,UITTERLINDEN A G,et al.Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA[J].Applied and Environmental Microbiology,1993,59(3):695-700.[10]KORBIE D J,MATTICK J S.Touchdown PCR for increased specificity and sensitivity in PCR amplification[J].Nature Protocols,2008,3(9):1452-1456.[11]THOMPSON J R,MARCELINO L A,POLZ M F.Heteroduplexes in mixed-template amplifications:Formation,consequence and elimination by ‘reconditioning PCR’[J].Nucleic Acids Reseach,2002,30(9):2083-2088.[12]OROSSICHLER M,GOMES N C,NEUBER G,et al.A new semi-nested PCR protocol to amplify large 18S rRNA gene fragments for PCR-DGGE analysis of soil fungal communities[J].Journal of Microbiological Methods,2006,65(1):63-75.[13]ZHANG J C,ZENG G M,CHEN Y N,et al.Effects of physico-chemical parameters on the bacterial and fungal communities during agricultural waste composting[J].Bioresource Technology,2011,102(3):2950-2956.[14]高丽倩,赵允格,许明祥,等.生物土壤结皮演替对土壤生态化学计量特征的影响[J].生态学报,2018,38(2):678-688.[15]王丽丽.晋陕蒙汾河排土场植物措施对复垦土壤养分和微生物的影响[D].北京:中国科学院大学(中国科学院教育部水土保持与生态环境研究中心),2018.[16]贺龙,李艳琴,李彬春,等.汾河不同植被复垦模式对土壤细菌群落结构的影响[J].环境科学,2017,38(2):752-759.[17]SUBRAHMANYAM G,HU H W,ZHENG Y M,et al.Response of ammonia oxidizing microbes to the stresses of arsenic and copper in two acidic alfisols[J].Applied Soil Ecology,2014,77(1):59-67.[18]SIMS A,GAJARAJ S,HU Z Q.Seasonal population changes of ammonia-oxidizing organisms and their relationship to water quality in a constructed wetland[J].Ecological Engineering,2012,40(3):100-107.[19]WOLSING M,PRIEM A.Observation of high seasonal variation in community structure of denitrifying bacteria in arable soil receiving artificial fertilizer and cattle manure by determining T-RFLP of nir gene fragments[J].Fems Microbiology Ecology,2004,48(2):261-271.[20]SHARMA S,ANEJA M K,MAYER J,et al.Diversity of transcripts of nitrite reductase genes (nir K and nir S) in rhizospheres of grain legumes[J].Applied and Environmental Microbiology,2005,71(4):2001-2007.[21]山西省统计局.2016年山西统计年鉴[M].太原:中国统计出版社,2015.[22]王若仪,李雅忠.山西省生态环境状况及变化趋势分析[J].科技创新与生产力,2016(5):20-22,25.[23]NICOL G W,LEININGER S,SCHLEPER C,et al.The influence of soil pH on the diversity,abundance and transcriptional activity of ammonia oxidizing archaea and bacteria[J].Environmental Microbiology,1979,10(11):2966-2978.[24]王如.汾河流域水资源生态环境的可持续发展研究[D].山西:太原科技大学,2013.[25]王浩,薛龙义.山西经济发展与生态环境协调发展探究[J].山西师范大学学报(自然科学版),2009,23(3):97-100.
相似文献/References:
[1]王 菲,王建宇,王幼奇.宁夏荒地压砂年限与土壤理化性质研究[J].北方园艺,2014,38(13):181.
WANG Fei,WANG Jian-yu,WANG You-qi.Study on Sand-mulching Years and Soil Physicochemical Properties of Wasteland in Ningxia[J].Northern Horticulture,2014,38(14):181.
[2]苏德喜,贾倩民.牧草品种与施肥对干旱区弃耕地土壤理化性质的影响[J].北方园艺,2014,38(11):153.
SU De-xi,JIA Qian-min.Effects of Different Forage Species and Fertilizer on Soil Physical and Chemical Properties in Abandoned Land of Arid Area[J].Northern Horticulture,2014,38(14):153.
[3]袁 勤,崔向新,乔 荣.砒砂岩区不同人工林对土壤理化性质的影响[J].北方园艺,2013,37(18):52.
YUAN Qin,CUI Xiang-xin,QIAO Rong.Effect of Different Artificial Forest Plantations on Soil Physical and Chemical Properties of Sandstone Areas[J].Northern Horticulture,2013,37(14):52.
[4]焦迎春,卢素锦.青海大通周边地区不同土壤结构微生物种群研究[J].北方园艺,2013,37(08):178.
JIAO Ying-chun,LU Su-jin.Study on Microbial Population in Different Soil Structures in Surrounding Areas of Datong of Qinghai[J].Northern Horticulture,2013,37(14):178.
[5]李旭青.乌兰县秸秆腐熟剂应用效果[J].北方园艺,2012,36(11):162.
LIXu-qing.ApplicationExperimentofStrawDecomposingInoculantsinWulan[J].Northern Horticulture,2012,36(14):162.
[6]郝宇,张艳馥,刘丽杰,等.扎龙湿地土壤重金属含量与土壤理化性质的相关性研究[J].北方园艺,2013,37(24):167.
HAO Yu,ZHANG Yan-fu,LIU Li-jie,et al.Study on Correlation Between Soil Properties and Heavy Metals Contents Isolated from Soils of Zhalong Wetlands[J].Northern Horticulture,2013,37(14):167.
[7]王亮,王夏楠,周正立,等.塔里木河中游典型样地土壤主要理化性质比较研究[J].北方园艺,2014,38(23):148.
WANG Liang,WANG Xia-nan,ZHOU Zheng-li,et al.Comparative Study on Soil Physicochemical Properties in the Middle Reaches of Tarim River[J].Northern Horticulture,2014,38(14):148.
[8]李海玲,曹忻,哈斯其美格,等.种植转基因番茄对温室土壤理化性质及养分的影响[J].北方园艺,2015,39(20):159.[doi:10.11937/bfyy.201520040]
LI Hailing,CAO Xin,HA Siqimeige,et al.Effect of Planting Transgenic Tomatoes on Soil Physicochemical Properties and Nutrient in Greenhouse[J].Northern Horticulture,2015,39(14):159.[doi:10.11937/bfyy.201520040]
[9]王立河,贾云超,王俊忠,等.营养平衡调节对缓解大蒜连作障碍的研究[J].北方园艺,2015,39(21):167.[doi:10.11937/bfyy.201521044]
WANG Lihe,JIA Yunchao,WANG Junzhong,et al.Study on Alleviating Continuous Cropping Obstacle of Garlic by Nutrient Balance[J].Northern Horticulture,2015,39(14):167.[doi:10.11937/bfyy.201521044]
[10]卢兴霞,刘艳,杨静慧,等.种植国槐和刺槐对滨海盐碱地理化性质的影响[J].北方园艺,2016,40(04):64.[doi:10.11937/bfyy.201604017]
LU Xingxia,LIU Yan,YANG Jinghui,et al.Effect of the Plantations of Sophoro joponica and Robinia pseudoacacia on Physical and Chemical Properties in Coastal Saline-alkali Soil[J].Northern Horticulture,2016,40(14):64.[doi:10.11937/bfyy.201604017]
备注/Memo
第一作者简介:李扬(1985-),女,博士,副教授,现主要从事生态环境修复等研究工作。E-mail:376227985@qq.com.责任作者:赵璐(1986-),女,博士,副教授,现主要从事水土资源与环境等研究工作。E-mail:xxjswlh@qq.com.基金项目:国家重点研发计划资助项目(2016YFC0400206);国家自然科学基金资助项目(51779161);2020年山西省重大科研资助项目。收稿日期:2020-09-26