TIAN Jia,WANG Hui,SUN Yu,et al.Effects of High Temperature Stress on Physiological and Biochemical Indexes of Apple Leaves[J].Northern Horticulture,2021,(10):28-34.[doi:10.11937/bfyy.20203286]
高温胁迫对苹果叶片生理生化指标的影响
- Title:
- Effects of High Temperature Stress on Physiological and Biochemical Indexes of Apple Leaves
- 文献标志码:
- A
- 摘要:
- 以四年生“王林”“嘎拉”盆栽苹果树为试材,采用人工模拟高温的方法,研究了高温胁迫对苹果叶片生理生化指标的影响,以期为苹果叶片热害的深入研究提供参考依据。结果表明:12 h时,“王林”新梢叶片的热害率和热害指数分别为27.96%和5.59,高于“嘎拉”新梢叶片的热害率(23.33%)和热害指数(4.67)。高温胁迫下,2个品种叶片叶绿素含量均表现出逐渐降低的趋势;随着高温时间的延长,2个品种叶片的净光合速率均逐渐下降,胞间CO2浓度逐渐升高,气孔导度和蒸腾速率均表现出先升高后降低的趋势,且在3 h时达到峰值。超氧阴离子产生速率、丙二醛含量和相对电导率则持续升高;可溶性蛋白质含量和超氧化物歧化酶、过氧化物酶活性均呈现出先升高后降低的规律,且在高温胁迫6 h达到峰值。
- Abstract:
- The four-year-old ‘Orin’ and ‘Gala’ potted apple trees were used as experimental materials,the method of artificially simulating high temperature was used,the effects of high temperature stress on the physiological and biochemical indexes of apple leaves were studied,in order to provide a reference for the indepth study of the thermal damage of apple leaves.The results showed that the heat damage rate and heat damage index of new shoot leaves of ‘Orin’ new shoot leaves were 27.96% and 5.59 at 12 hours,which were higher than those of ‘Gala’,23.33% and 4.67.Under high temperature stress,the chlorophyll content of the leaves of the two cultivars showed a gradual decrease.With the extension of high temperature time,the net photosynthetic rate of the leaves of the two cultivars gradually decreased,the intercellular CO2 concentration gradually increased,and the stomatal conductance and transpiration rate both increased first and then decreased,and reached the peak at 3 hours.Production rate of superoxide anion,malondialdehyde content and relative conductivity continued to increase.The soluble protein content and the activities of superoxide dismutase and peroxidase all showed a pattern of first increasing and then decreasing,and reached the peak at 6 hours of high temperature stress.
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备注/Memo
第一作者简介:田佳(1995-),女,硕士研究生,研究方向为农艺与种业。E-mail:secrettj@163.com.责任作者:徐继忠(1964-),男,博士,教授,现主要从事果树栽培生理与分子生物学等研究工作。E-mail:xjzhxw@126.com.基金项目:国家苹果产业技术体系保定综合实验站资助项目(CARS-27)。收稿日期:2020-08-04