ZHAO Ying,MA Xiaoying,CHEN Xun,et al.Correlation Analysis of Nutrient Components and Enzyme Activity of Cordyceps militaris Cultivated in Different Substrates[J].Northern Horticulture,2021,(08):99-105.[doi:10.11937/bfyy.20202527]
不同基质栽培蛹虫草营养成分与酶活的相关性分析
- Title:
- Correlation Analysis of Nutrient Components and Enzyme Activity of Cordyceps militaris Cultivated in Different Substrates
- 文献标志码:
- A
- 摘要:
- 以小麦、大米和柞蚕蛹为培养基质的子实体为试材，对比其多糖和蛋白质含量，并测定漆酶、纤维素酶、淀粉酶、蛋白酶、滤纸酶、多酚氧化酶活性，以此分析各胞内酶活性与多糖和蛋白质含量的相关性，以期为获得高蛋白和高多糖的蛹虫草子实体栽培提供参考依据。结果表明：以柞蚕蛹为栽培基质的蛹虫草中的多糖和蛋白质含量最高。利用双重检验分析发现蛹虫草子实体中多糖的累积与纤维素酶活性呈极显著正相关，与蛋白酶活性呈显著负相关。蛹虫草子实体中蛋白质的累积与淀粉酶活性呈极显著正相关，与多酚氧化酶活性呈极显著负相关。
- Abstract:
- Cordyceps militaris fruiting body was used to compare the polysaccharide and protein content of Cordyceps militaris fruiting body in wheat,rice and silkworm chrysalis as the medium,and the enzyme activity indexes such as laccase,cellulase,amylase,protease,filter paper enzyme and polyphenol oxidase,etc,so as to analyze the correlation between intracellular enzyme activity and polysaccharide and protein content,in order to provide reference for the cultivation of Cordyceps militaris fruiting body with high protein and polysaccharide.The results showed that polysaccharide and protein content in Cordyceps militaris were all higher than those in wheat and rice matrix.Also,as the double test analysis showed,the accumulation of polysaccharides in Cordyceps militaris fruiting body was significantly positively correlated with cellulase activity,and negatively correlated with protease activity.The accumulation of protein in Cordyceps militaris fruiting body was significantly positively correlated with amylase activity and negatively correlated with polyphenol oxidase activity.
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备注/Memo
第一作者简介：肇莹（1981-），女，硕士，副研究员，现主要从事蛹虫草遗传育种和栽培等研究工作。E-mail:zhaoying_226@163.com.责任作者:肖军（1974-），女，硕士，研究员，现主要从事食用菌育种和栽培等研究工作。E-mail:xiaojun20002@163.com.基金项目：辽宁省科技计划资助项目（2019JH5/10100009，2020JH5/10400085）；沈阳市科技计划资助项目（19-302-3-08）；辽宁省重点研发计划资助项目（2020JH2/10200013）。收稿日期：2020-06-17