YANG Yunying,WANG Tingqin,ZHOU Zihan.Extraction and Stability of Purple Carnation Pigment[J].Northern Horticulture,2021,(02):80-84.[doi:10.11937/bfyy.20201166]
紫色康乃馨色素提取及其稳定性
- Title:
- Extraction and Stability of Purple Carnation Pigment
- Keywords:
- purple Dianthus caryophyllus Linn.; pigment; extraction; stableness
- 文献标志码:
- A
- 摘要:
- 以紫色康乃馨切花为试材，采用单因素预试验获得色素提取的最佳条件后经正交实验，研究了紫色康乃馨色素最佳组合提取条件及各种外界条件对吸光度、溶液颜色、色素稳定性等的影响，以期为紫色素提取提供借鉴。结果表明：紫色康乃馨色素最佳组合提取条件为pH 2、浓度为60%的柠檬酸溶液，按1∶1 500 g?mL-1的料液比，在40 ℃恒温提取180 min；该色素易溶于柠檬酸和水，为水溶性色素，并在可见光区340 nm时有最大吸收峰。而碱性环境和光照环境显著影响其稳定；Cu2+、Fe3+离子对该色素影响较其它金属离子如Al3+、Ca2+、Zn2+等更大；氧化剂对其减色作用显著；但色素耐还原性强；可溶性淀粉及还原剂对其有一定的增色作用。
- Abstract:
- Taking cut-flower purple carnation as the test material，a single-factor pre-test was used to obtain the best conditions for pigment extraction.Through orthogonal experiments，the best combination extraction conditions of purple carnation pigment and the influence of various external conditions on the absorbance，solution color and pigment stability were studied,in order to provide reference for extracting purple pigment.The results showed that the best combination extraction conditions for purple carnation pigments were pH 2，60% citric acid solution，and solid-liquid ratio of the 1∶1 500 g?mL-1 solution was extracted at a constant temperature of 40 ℃ for 180 minutes.The pigment was easily soluble in citric acid and water，was a water-soluble pigment，and had a maximum absorption peak at 340 nm in the visible light region.Alkaline environment and light environment significantly affected its stability.Cu2+ and Fe3+ ions affected the pigment more than other metal ions such as Al3+，Ca2+，Zn2+ and so on.The oxidant had a significant effect on reducing its color，but the pigment had strong reduction resistance.Soluble starch and reducing agents had a certain color-increasing effect on it.
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备注/Memo
第一作者简介：杨运英（1974-），女，博士，副教授，现主要从事观赏园艺植物的生产与栽培生理及基因工程和植物的开发利用等工作。E-mail:yyying2@126.com.基金项目：广东省特色创新类（自然科学）资助项目（2017GKTSCX024）。收稿日期：2020-03-24