WANG Guichao,WANG Shaozu,LI Zhengzhou,et al.Cloning and Molecular Markers Development of CmFT in Melon（Cucumis melo L.）[J].Northern Horticulture,2020,44(19):16-22.[doi:10.11937/bfyy.20200271]
甜瓜CmFT基因的克隆及其分子标记开发
- Title:
- Cloning and Molecular Markers Development of CmFT in Melon（Cucumis melo L.）
- Keywords:
- melon; flowering; CmFT; homologous clone; molecular markers
- 文献标志码:
- A
- 摘要:
- 以“HM1-1”“MS-5”甜瓜品种为试材，采用同源克隆的方法得到甜瓜FT同源基因，命名为CmFT，通过进化树分析、实时荧光定量方法以及分子标记辅助选择育种等方法初步验证了CmFT在甜瓜花发育过程中的作用。结果表明：该基因全长3 843 bp，CDS区为534 bp，编码178个氨基酸。在24个甜瓜品种中开展分子标记辅助筛选准确率达87.5%。该标记与甜瓜开花性状相关，可用于甜瓜早花育种的辅助选择，研究结果对甜瓜分子育种与开花基因调控提供了参考依据。
- Abstract:
- ‘HM1-1’ and ‘MS-5’ melon varieties were used as materials，and the melon FT homologous gene was cloned by homologous cloning method，named CmFT.Through phylogenetic tree analysis，real-time fluorescence quantitative methods，and molecular marker-assisted selection breeding methods，the role of CmFT in the development of melon flowers was preliminarily verified.The results showed that the gene was 3 843 bp in length and 534 bp in CDS，encoding 178 amino acids.The accuracy of molecular marker-assisted screening in 24 melon varieties reached 87.5%.The marker was related to the flowering traits of melon and could be used for auxiliary selection in early flowering breeding of 〖JP2〗melon.The results provided a theoretical basis for molecular breeding and flowering gene regulation of melon.
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备注/Memo
第一作者简介：王桂超（1995-），男，硕士研究生，研究方向为甜瓜分子育种。E-mail:wangguichaovip@163.com.责任作者：盛云燕（1979-），女，博士，教授，现主要从事甜瓜分子育种等研究工作。E-mail:shengyunyan12345@163.com.基金项目：国家自然科学基金资助项目（31772330）；黑龙江省自然科学基金资助项目(C2017054)；黑龙江八一农垦大学校级重点培育资助项目（XA-2017-01）。收稿日期：2019-11-27