[1]殷亚蕊,武军凯,王海静,等.桃果实PpTIFY10b基因的克隆与表达分析[J].北方园艺,2020,44(22):23-30.[doi:10.11937/bfyy.20200189]
　YIN Yarui,WU Junkai,WANG Haijing,et al.Cloning and Expression Analysis of [STBX]PpTIFY10b[STBZ] Gene in Peach Fruit[J].Northern Horticulture,2020,44(22):23-30.[doi:10.11937/bfyy.20200189]
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桃果实PpTIFY10b基因的克隆与表达分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第44卷 期数: 2020年22 页码: 23-30 栏目: 出版日期: 2020-11-30
Title:
Cloning and Expression Analysis of [STBX]PpTIFY10b[STBZ] Gene in Peach Fruit
作者:
殷亚蕊; 武军凯; 王海静; 宋立琴; 肖啸; 张立彬
（河北科技师范学院 园艺科技学院，河北 秦皇岛 066600）
Author(s):
YIN Yarui; WU Junkai; WANG Haijing; SONG Liqin; XIAO Xiao; ZHANG Libin
(College of Horticulture Science and Technology，Hebei Normal University of Science and Technology，Qinhuangdao,Hebei 066600)
关键词:
桃; [STBX]PpTIFY10b[STBZ]基因; 克隆; 组织表达
Keywords:
Prumus persica; [STBX]PpTIFY10b[STBZ] gene; cloning; tissue expression
DOI:
10.11937/bfyy.20200189
文献标志码:
A
摘要:
采用同源克隆方法从‘京红’桃及其晚熟芽变果实中克隆出[STBX]PpTIFY10b[STBZ]基因，初步分析了该基因的理化性质，并利用荧光定量PCR（RT-PCR）分析了该基因在‘京红’桃以及其突变体中的表达情况，以期揭示桃果实成熟机制提供基本数据支持。结果表明：该基因开放阅读框为639 bp，编码213个氨基酸，等电点为9.04，为以丝氨酸为主的亲水性不稳定蛋白。同源性比对和结构分析表明，该基因与其它植物TIFY10b蛋白具有较高的相似性，其中与梅的一致性最高，均含有1个TIFY功能结构域和1个CCT基序。荧光定量PCR结果表明，该基因在桃及其晚熟芽变果实的生理成熟期表达量最高，但二者的表达规律有显著差异，即晚熟芽变在S1、S4时期的基因表达量上调，且明显高于‘京红’，表明[STBX]PpTIFY10b[STBZ]基因参与了果实成熟调控。该研究为了解桃果实成熟期调控机制提供了新颖的理论数据。
Abstract:
In order to further clarify the resistance mechanism of peach,determine the resistance-related gene [STBX]PpTIFY10b[STBZ]，preliminarily explore the function of this gene in peach growth，[STBX]PpTIFY10b[STBZ] gene was cloned from the fruits of ‘Jinghong’ peach and its mutants by homologous cloning technology，and its quantitative expression and bioinformatics analysis were carried out.Flourescence quantitative PCR was used to anylyze the expression of the gene in ‘Jinghong’ peach and its mutants and provide data support for further revealing the resistance mechanism of peach.The results showed that the open reading frame of the gene was 639 bp，encoding 213 amino acids，and theoretical pI was 9.04.It was a hydrophilic unstable protein mainly composed of serine.Homology comparison and structural analysis revealed that TIFY10b protein had high similarity with other plant proteins，and had the highest consistency with Prunus mume.It contained a TIFY functional domain and a CCT motif.The results of quantitative fluorescence PCR showed that the expression of the gene was the higherin the physiological maturity stage of the two peaches，but there was a significant difference between them.That meant the gene expression of bud mutant lines was up-regulated at the S1 and S4 stages，and was significantly higher than that of ‘Jinghong’ peaches.The results of this study provided biological information support for understanding the research of peach in abiotic stress.

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备注/Memo

第一作者简介：殷亚蕊(1993-)，女，硕士研究生，研究方向为果树遗传育种与分子生物学。E-mail:1174932343@qq.com.责任作者：张立彬（1962-），男，硕士，教授，硕士生导师，现主要从事果树遗传育种、果树种质资源与分子生物学等研究工作。E-mail：zhanglibin9364@163.com.基金项目：国家自然科学基金资助项目（31572093）；河北省自然科学基金资助项目（C2016407110）；河北省重点研发计划基金资助项目（16226313D）。收稿日期：2020-01-12

更新日期/Last Update: 2021-02-02