[1]周锦业,崔学强,李秀玲,等.低温胁迫过程中兜兰内源激素的动态变化[J].北方园艺,2020,44(21):50-55.[doi:10.11937/bfyy.20194676]
　ZHOU Jinye,CUI Xueqiang,LI Xiuling,et al.Dynamic Changes of Endogenous Hormones in Paphiopedilum Orchids During Low Temperature Stress[J].Northern Horticulture,2020,44(21):50-55.[doi:10.11937/bfyy.20194676]
点击复制

低温胁迫过程中兜兰内源激素的动态变化

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第44卷 期数: 2020年21 页码: 50-55 栏目: 出版日期: 2021-11-15
Title:
Dynamic Changes of Endogenous Hormones in Paphiopedilum Orchids During Low Temperature Stress
作者:
周锦业; 崔学强; 李秀玲; 范继征; 卜朝阳; 张自斌
（广西农业科学院 花卉研究所，广西 南宁 530007）
Author(s):
ZHOU Jinye; CUI Xueqiang; LI Xiuling; FAN Jizheng; BU Zhaoyang; ZHANG Zibin
(Flower Research Institute,Guangxi Academy of Agricultural Sciences,Nanning，Guangxi 530007)
关键词:
兜兰; 内源激素; 低温胁迫; 生长调节剂
Keywords:
Paphiopedilum; endogenous hormones; low temperature stress; growth regulator
DOI:
10.11937/bfyy.20194676
文献标志码:
A
摘要:
以黄花系肉饼兜兰、红花系肉饼兜兰和杏黄兜兰为试材，采用高效液相色谱法在昼20~25 ℃/夜16~18 ℃条件下持续处理30 d，测定其内吲哚乙酸（IAA）、赤霉素（GA3）、脱落酸（ABA）和玉米素核苷（ZR）含量变化，研究低温处理下兜兰叶片中内源激素的变化情况。结果表明：低温处理下不同兜兰的4种内源激素在植株遭遇低温时均能迅速做出反应，通过内源激素的变化使植株迅速适应环境的变化，其中肉饼类兜兰其内IAA含量持续增加，而杏黄兜兰IAA含量则出现降低的现象；而就GA3和ZR变化情况而言，除了杏黄兜兰GA3出现先增后降再增的情况，其它均为先增后减；3种兜兰内ABA含量变化则各不相同，但是初期均为含量突增。因此结合兜兰不同生长期以及花芽分化不同时期其内源激素的变化规律，从而掌握通过温度控制兜兰花期的方法具有可行性。
Abstract:
To study the changes of endogenous hormones in Paphiopedilum leaves under low temperature treatment,P.armeniacum,yellow flower series and red flower series of complex standard hybrids of Paphiopedilum were used as the experimental materials,with the continuous temperature treatment for 30 days at the conditions of daytime temperature 20-25 ℃ and night temperature 16-18 ℃.The changes of contents of IAA,GA3,ABA and ZR were determined by high performance liquid chromatography.The results showed that the four endogenous hormones of different Paphiopedilum species responded quickly when the plants were exposed to the control temperature,and the plants adapted rapidly to the changes of the environment.Among them,the contents of IAA of the two complex standard hybrids species were increased continuly,and the content of IAA of P.armeniacum was reduced.The content change of GA3 and ZR of the two complex standard hybrids species were increased at first and then were decreased,and ZR content change of P.armeniacum was similar to these.The GA3 content change of P.armeniacum were increased at first and then increased,ABA contents changes of the three Paphiopedilum species were different,but the contents were suddenly increased at the beginning.Therefore,it was feasible to control the flowering period of Paphiopedilum species by combining the changes of endogenous hormones at different development stages.

参考文献/References:

[1]李秀玲,王晓国,李春牛,等.基于灰色关联分析方法评价13种野生兜兰的迁地保护适应性[J].植物科学学报，2015,33(3):326-335.[2]曾宋君,陈砚,吴坤林,等.国外兜兰属植物的引种和栽培初报[J].中国野生植物资源，2013,32(5):62-68.[3]王贞,丛磊,刘燕.兜兰属植物研究现状[J].林业科学，2006,42(7):113-119.[4]胡琦敏,李勇毅,黄云峰,等.海伦兜兰的无菌播种与快速繁殖[J].植物生理学报，2016,52(9):1443-1448.[5]李秀玲,黄昌艳,宋倩,等.同色兜兰的非共生萌发与快速繁殖研究[J].植物科学学报，2016,34(1):127-134.[6]NG C Y,SALEH N M.In vitro propagation of Paphiopedilum orchid through formation of protocorm-like bodies[J].Plant Cell Tissue and Organ Culture,2011,105(2):193-202.[7]VU Q L,NGUYEN P H,NGUYEN B N,et al.Ex vitro and in vitro Paphiopedilum delenatii Guillaumin stem elongation under light-emitting diodes and shoot regeneration via stem node culture[J].Acta Physiologiae Plantarum,2015,37(7):136.[8]曾宋君,陈之林,吴坤林,等.兜兰无菌播种和组织培养研究进展[J].园艺学报,2007,34(3):793-796.[9]张雪,张毓,陈红岩.杏黄兜兰和硬叶兜兰在北京的催花比较试验[J].中国园艺文摘,2012,28(6):18-19+29.[10]张娟娟,严宁,胡虹.三种兜兰属植物种子发育过程及其与无菌萌发的关系[J].植物分类与资源学报,2013,35(1):33-40.[11]张英杰,刘学庆,张京伟,等.兜兰叶片结构与抗旱性关联的研究[J].安徽农业大学学报,2017,44(6):1104-1111.[12]陈业,石建明,沈文华,等.23种中国兜兰属植物亲缘关系的ISSR分析[J].西南大学学报(自然科学版)，2013,35(6):15-21.[13]LEE Y,YAP W J,IZAN S,et al.Satellite DNA in Paphiopedilum subgenus Parvisepalum as revealed by high-throughput sequencing and fluorescent in situ hybridization[J].BMC Genomics,2018,19(1):578-589.[14]HOU N,WANG G,ZHU Y,et al.The complete chloroplast genome of the rare and endangered herb Paphiopedilum dianthum (Asparagales:Orchidaceae)[J].Conservation Genetics Resources,2017,10(4):1-4.[15]李宗艳,管名媛,李静,等.基于ISSR的硬叶兜兰居群遗传多样性研究[J].西北植物学报,2016,36(7):1351-1356.[16]朱亚艳,王港,侯娜,等.贵州南部野生兜兰SRAP遗传多样性分析[J].西南林业大学学报,2017,37(1):10-14.[17]LINCOLN T,EDUARDO Z.植物生理学[M].4版.北京:科学出版社,2009.[18]黄国林,肖远志,李卫东,等.兰花花期调控研究进展[J].湖南农业科学,2015(5):146-148，151.[19]邵莉楣,孟小雄,植物生长调节剂应用手册[M].北京:金盾出版社,2010.[20]田小霞,孟林,毛培春,等.低温条件下不同抗寒性薰衣草内源激素的变化[J].植物生理学报,2014,50(11):1669-1674.[21]王兴,于晶,杨阳,等.低温条件下不同抗寒性冬小麦内源激素的变化[J].麦类作物学报,2009,29(5):827-831.[22]孙红梅,廖浩斌,刘盼盼,等.不同成花量金花茶花果期果枝叶内源激素的变化[J].广西植物,2017,37(12):1537-1544.[23]吴尚,马履一,段劼,等.文冠果花期和果期内源激素的动态变化规律[J].西北农林科技大学学报(自然科学版),2017,45(4):111-118.[24]刘海卿,方园,武军艳,等.低温胁迫下内源ABA、GA及比值对白菜型和甘蓝型冬油菜抗寒性的响应[J].中国生态农业学报,2016,24(11):1529-1538.[25]陶兴林,朱惠霞,胡立敏,等.花椰菜温敏雄性不育系GS-19花蕾发育过程中内源激素的动态变化分析[J].核农学报,2017,31(8):1626-1632.[26]陈睿,鲜小林.不同温度和光强对高山杜鹃催花期内源激素的影响[J].西南农业学报,2016,29(10):2341-2345.[27]刘学庆,孙纪霞,丁朋松,等.低温胁迫对蝴蝶兰内源激素的影响[J].江西农业大学学报,2012,34(3):464-469.[28]倪俊霞,李冠真,史莹华,等.温度对不同秋眠型紫花苜蓿光敏色素和内源激素的影响[J].草地学报,2013,21(4):708-713.[29]周越,范幸龙,周子方,等.夜温对不同品种烟苗顶芽主要内源激素含量的影响[J].中国农业气象,2015,36(4):489-496.

相似文献/References:

[1]程 华,李琳玲,袁红慧,等.内源激素含量变化与板栗花芽分化关系研究[J].北方园艺,2013,37(22):5.
　CHENG Hua,LI Lin-ling,YUAN Hong-hui,et al.Study on the Relationship of Changes of the Endogenous Hormone Content in Castanea mollissima and Bud Differentiation[J].Northern Horticulture,2013,37(21):5.
[2]李琳玲,程 华,吴聪华,等.SPE-HPLC法同时分离测定板栗花芽中四种内源激素的研究[J].北方园艺,2014,38(01):22.
　LI Lin-ling,CHENG Hua,WU Cong-hua,et al.Simultaneous Determination of 4 Endogenous Hormones in Chinese Chestnut Floral Bud by Solid Phase Extraction-High Performance Liquid Chromatography[J].Northern Horticulture,2014,38(21):22.
[3]陈忠,董延龙,李岩.鹿蹄草在自然越冬过程中内源激素的变化研究[J].北方园艺,2013,37(13):181.
　CHEN Zhong,DONG Yan-long,LI Yan.Study on Changes of Reendogenous Hormones During Natural Winter of ?Pylora rotundifolia[J].Northern Horticulture,2013,37(21):181.
[4]隗晓雯,郭静,王菲,等.不同矮化砧木叶片酶活性与内源激素含量的差异[J].北方园艺,2013,37(14):16.
　WEI Xiao-wen,GUO Jing,WANG Fei,et al.Difference of Enzyme Activity and the Content of Endogenous Hormones in the Leaves of Different Apple Dwarfing Rootstocks[J].Northern Horticulture,2013,37(21):16.
[5]马艳萍,吕新刚,马惠玲,等.低温对鲜核桃贮藏效果及胚芽内源激素变化的影响[J].北方园艺,2013,37(05):134.
　MA Yan-ping,LV Xin-gang,MA Hui-ling,et al.Effect of Low-temperature on Preservation and Variation of Endogenous Hormones in Embryo of Fresh Walnut[J].Northern Horticulture,2013,37(21):134.
[6]孙伟,高佳缘,焦奎宝,等.“绥李3号”花芽萌发过程中内源激素含量变化研究[J].北方园艺,2013,37(19):33.
　SUN Wei,GAO Jia-yuan,JIAO Kui-bao,et al.Study on the Changes of Endogenous Hormones Content in Flower Buds of ‘Suili 3’[J].Northern Horticulture,2013,37(21):33.
[7]焦竹青,王振兴,许培磊,等.山葡萄雄株花蕾性别转换的形态观察及其内源激素的变化[J].北方园艺,2012,36(16):5.
　JIAO Zhu-qing,WANG Zhen-xing,XU Pei-lei,et al.Morphological and Endogenous Hormones Changes on Sex Conversion of Male Vitis amurensis [J].Northern Horticulture,2012,36(21):5.
[8]覃文渊,覃文更,覃国乐,等.河池市兜兰属植物资源及其保护利用初探[J].北方园艺,2012,36(15):103.
　QIN Wen-yuan,QIN Wen-geng,QIN Guo-le,et al.A Preliminary Study on Strategies of Protection and Utilizationof Paphiopedilum Genus in Hechi[J].Northern Horticulture,2012,36(21):103.
[9]步佳佳,胡玉林,魏长斌,等.利用HPLC法对香蕉根中四种内源激素测定方法的优化[J].北方园艺,2012,36(08):30.
　BU Jia-jia,HU Yu-lin,WEI Chang-bin,et al.The Optimum of HPLC Conditions of Four Kinds of Endogenous Hormones in the Roots of Banana[J].Northern Horticulture,2012,36(21):30.
[10]杨光穗,黄素荣,王存,等.黄花美冠兰花芽分化过程中假鳞茎内源激素含量变化的研究[J].北方园艺,2012,36(23):75.
　YANG Guang-sui,HUANG Su-rong,WANG Cun,et al.Study on Changes of Endogenous Hormone Contents in Pseudobulbs During the Floral Bud Differentiation of Eulophia flava[J].Northern Horticulture,2012,36(21):75.

备注/Memo

第一作者简介：周锦业（1987-），男，硕士，高级工程师，现主要从事园林植物生物技术和品种选育等研究工作。E-mail：ahzhoujy@163.com.责任作者：李秀玲（1987-），女，硕士，副研究员，现主要从事花卉遗传育种等研究工作。E-mail：congzxiao@163.com.基金项目：广西重点研发计划资助项目（桂科AB16380089,桂科AB16380061）；南宁市技术创新引导专项资助项目（20175031-5,20185067-2）；广西农业科学院科技发展基金资助项目（桂农科2016JM04,桂农科2020YM123）；广西创新驱动发展专项资金资助项目（桂科AA17204046-1,桂科AA17204045-6）。收稿日期：2019-12-11

更新日期/Last Update: 2021-01-21