YANG Yang,WANG Yajuan,YIN Fating,et al.Effects of Saline-Alkali Stress on Physiology and Photosynthetic Characteristics of Rape Seedlings[J].Northern Horticulture,2020,44(15):1-8.[doi:10.11937/bfyy.20194532]
盐碱胁迫对油菜苗期生理及光合特性的影响
- Title:
- Effects of Saline-Alkali Stress on Physiology and Photosynthetic Characteristics of Rape Seedlings
- Keywords:
- rapeseed seedling stage; saline-alkali stress; osmotic adjustment; protective enzyme; photosynthetic gas exchange parameters
- 文献标志码:
- A
- 摘要:
- 以“华油杂62号”油菜为试材，采用盆栽方法，研究了复合盐碱胁迫对“华油杂62号”油菜苗期生理及光合特性的影响，以期为油菜在盐碱地的种植提供参考依据。结果表明：复合盐碱胁迫下，油菜幼苗叶片中可溶性糖、脯氨酸含量在重度处理时显著低于轻度处理，且苗后期含量高于苗前期；其可溶性蛋白质含量在苗前期中、重度处理显著高于轻度处理，且苗后期低于苗前期。油菜幼苗叶片中保护酶活性有不同程度的降低趋势，但各处理间差异不显著，且超氧化物歧化酶（SOD）、过氧化物酶（POD）活性在苗后期低于苗前期，过氧化氢酶（CAT）活性在苗后期高于苗前期。随着盐碱程度增大，油菜幼苗净光合速率(Pn)、蒸腾速率(Tr)、胞间CO2浓度(Ci)、气孔导度(Gs)均呈现出先降低后升高的趋势。复合盐碱胁迫对油菜苗期渗透调节物质（可溶性糖、脯氨酸、可溶性蛋白质）含量、酶（SOD、POD、CAT）活性以及光合参数（净光合速率(Pn)、蒸腾速率(Tr)、胞间CO2浓度(Ci)、气孔导度(Gs)）具有抑制作用，且随着盐碱程度的增大，抑制作用增强；油菜苗前期抑制程度大于苗后期，在苗后期油菜光合能力减弱，体内酶活性降低，但其可通过合成渗透调节物质来适应逆境。
- Abstract:
- Huayouza 62’ was used as the test material，and the potting method was used to study the effect of compound saline-alkali stress on the seedling physiology and photosynthetic characteristics of ‘Huayouza 62’ rape，in order to provide a theoretical basis for the cultivation of rape in saline-alkali land.The results showed that under the compound saline-alkali stress, the soluble sugar and proline content in the leaves of rape seedlings were significantly lower in the severe treatment than in the mild treatment, and the content in the late seedling stage was higher than that in the early stage; the soluble protein content was moderate to severe in the early stage The treatment was significantly higher than the light treatment, and the late seedling stage was lower than the early seedling stage. The protective enzyme activities in the leaves of rape seedlings decreased to varying degrees, but the differences were not significant among the treatments.The activities of SOD and POD were lower than those of the pre-emergence stage in the late seedling stage，and the activity of CAT was higher than that of the pre-emergence stage in the later seedling stage.As the salinity increased，the net photosynthetic rate (Pn)，transpiration rate (Tr)，intercellular CO2 concentration (Ci)，and stomatal conductance (Gs) of rapeseed seedlings all showed a trend of decreasing first and then increasing.Compound saline-alkali stress on the content of osmotic adjustment substances (soluble sugar，proline，soluble protein)，enzyme activity (SOD，POD，CAT) and photosynthetic parameters (net photosynthetic rate (Pn)，transpiration rate (Tr)，intercellular CO2 concentration (Ci)，stomatal conductance (Gs)) had an inhibitory effect，and as the degree of salinity increases，the inhibitory effect was enhanced;the degree of inhibition in the early stage of rapeseed was greater than that in the late stage of seedling，and the photosynthetic capacity of rapeseed was weakened in the late stage of seedling，enzyme activity in the body was reduced，but it could adapt to adversity by synthesizing osmotic adjustment substances.
参考文献/References:
[1]PAZ R C,REINOSO H,ESPASABDIN F D，et al.Akaline，saline and mixed saline alkaline induce physiologicaland morphoanatomical changes in Lotus tenuis shoots[J].Plant Biol，2014，16(6):1042-1049.[2]YANG J Y，ZHENG W，TIAN Y，et al.Effects of various mixed salt-alkaline stresses on growth，photosynthesis，and photosynthetic pigment contrations of Medicago ruthenica seedlings[J].Photosynthetica，2011，49(2):275-284.[3]孙璐,周宇飞,李丰先,等.盐胁迫对高粱幼苗光合作用和荧光特性的影响[J].中国农业科学,2012,45(16):3265-3272.[4]李子英,丛日春,杨庆山,等.盐碱复合胁迫对柳树幼苗生长和渗透调节物质含量的影响[J].生态学报,2017,37(24):1-8.[5]张哲,殷艳,刘芳,等.我国油菜多功能开发利用现状及发展对策[J].中国油料作物学报,2018,40(5):618-623.[6]黎咏蜀.饲用油菜栽培技术及营养价值研究[D].重庆:西南大学,2014.[7]郭丛阳,王天河,杨文元,等.河西地区麦后复种饲用(绿肥)油菜栽培技术及效益分析[J].草业科学,2008,25(3):90-92.[8]KAISER M，ELLERBROCK R H.Functionalcharacterization of soilorganic matter fractions different in solubility originating from a long term field experiment[J].Geoderma,2005,127(3-4):196-206.[9]刘奕媺,于洋,方军.盐碱胁迫及植物耐盐碱分子机制研究[J].土壤与作物,2018,7(2):201-211.[10]毛恋,芦建国,江海燕.植物响应盐碱胁迫的机制[J].分子植物育种,2020，18(10):3441-3448.[11]石连旋.松嫩不同盐碱化羊草草甸草原羊草光合及逆境生理生态特性研究[D].长春:东北师范大学,2007.[12]佚名.油菜品种华油杂62[J].湖北农业科学,2010,49(11):2770.[13]中国科学院上海植物生理研究所.现代植物生理学实验指南[M].北京:科学技术出版社,1999.[14]孔祥生,易现峰.植物生理学实验技术[M].北京:中国农业出版社,2008.[15]王学奎.植物生理生化实验原理和技术[M].北京:高等教育出版社,2006.[16]王志强,吴翠云,杨哲,等.盐碱胁迫对酸枣幼苗生长及生理生化特性的影响[J].干旱地区农业研究,2018,36(2):153-160.[17]董静,魏福,友刑锦城,等.马齿苋幼苗对盐碱胁迫的生理响应[J].江苏农业科学,2019(13):1002-1302.[18]CAPULA-RODRGUEZ R,VALDEZ-AGUILAR L A,CARTMILL D L,et al.Supplementary calcium and potassium improve the response of tomato (Solanum lycopersicum L.) to simultaneous alkalinity，salinity，and boron stress,communications in soil[J].Science and Plant Analysis,2016,47(4):505-507.[19]SINGH N,BHATLA S C.Nitric oxide and iron modulate heme oxygenase activity as a long distance signaling response to saltstress in sunflower seedling cotyledons[J].Nitric Oxide,2016,53:54-64.[20]徐田军,董志强,兰洪亮,等.低温胁迫下聚糠萘合剂对玉米幼苗光合作用和抗氧化酶活性的影响[J].作物学报,2012,38(2):352-359.[21]刘铎,丛日春,高卫东,等.盐碱胁迫对柳树抗氧化酶的影响[J].水土保持通报,2017,37(5):53-57.[22]李兵兵,魏小红,徐严.麻花秦艽种子休眠机制及破除方法[J].生态学报,2013,33(15):4631-4638.[23]乔枫,耿贵工.盐碱胁迫对沙棘种子萌发及幼苗抗氧化酶活性的影响[J].东北林业大学学报,2012,40(2):17-19.[24]陈涛,王贵美,沈伟伟,等.盐胁迫对红麻幼苗生长及抗氧化酶活性的影响[J].植物科学学报,2011,29(4):493-501.[25]SULTANA N,IKEDA T,ITOH R.Effect of NaCl salinity on photosynthesis and dry matter accumulation in developing rice grains[J].Environmental and Experimental Botany,1999,42(3):211-220.[26]WEI Y,XU X,TAO H,et al.Growth performance and physiological response in the halophyte Lycium barbarum grown at salt-affected soil[J].Annals of Applied Biology,2006,149(3):263-269.[27]YANG C W，XU H H，WANG L L，et al.Comparative effects of salt-stress and alkali-stress on the growth，photosynthesis,soluteaccumulation,and ion balance of barley plants[J].Photosynthetica，2009，47(1):79-86.[28]李辛,赵文智.荒漠区植物雾冰藜光合特性对混合盐碱胁迫的响应[J].生态学报,2018,38(4):1183-1193.[29]FARQUHAR G D,SHARKEY T D.Stomatal conductance and photosynthesis[J].Annual Review of Plant Physiology,1982,33:172-345.[30]郭燕枝,杨雅伦,孙君茂.我国油菜产业发展的现状及对策[J].农业经济,2016(7):44-46.
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备注/Memo
第一作者简介：杨洋（1992-），女，硕士研究生，研究方向为绿洲农业生态。E-mail:1321349533@qq.com.责任作者：张凤华（1970-），女，博士，教授，现主要从事干旱区绿洲农业生态等研究工作。E-mail:zfh2000@126.com.基金项目：兵团重大计划资助项目（2018AA005）；兵团科技合作计划资助项目（2020BC001）。收稿日期：2019-12-02