LIU Yang,LU Kaizheng,BIAN Shaojie,et al.Analysis of Differentially Expressed Gene Profile in the Root of Walnut Under Natural Temperature Regulation in the Field[J].Northern Horticulture,2020,44(17):30-38.[doi:10.11937/bfyy.20194278]
田间自然温度调控下核桃根系差异表达基因分析
- Title:
- Analysis of Differentially Expressed Gene Profile in the Root of Walnut Under Natural Temperature Regulation in the Field
- Keywords:
- walnut; root; natural temperature regulation; transcriptomes; differentially expressed genes
- 文献标志码:
- A
- 摘要:
- 以十五年生‘绿岭’核桃树为试材，采用高通量转录组测序的方法，研究了不同地温（地温16.4 ℃，W0；地温21.7 ℃，W1；地温27.1 ℃,W2）对核桃根系基因表达量的影响，以期挖掘与温度调控相关的功能基因，探明自然温度调控下核桃根系的应答反应的分子机制。结果表明：W1vsW0、W2vsW0和W2vsW1显著上调基因分别为430、2 110、2 385个，显著下调基因分别为247、2 494、3 015个。GO 富集分析表明，W1vsW0、W2vsW0和W2vsW1的差异表达基因均主要富集在生物学过程和分子功能两大类别，共同显著富集的GO类别有氨基糖分解代谢过程、单体生物合成过程、几丁质分解过程、含氨基葡萄糖化合物分解代谢过程、氧化还原过程和氧化还原酶活性；但W1vsW0的差异基因显著富集的分子功能多与物质的合成代谢相关，而W2vsW1的差异基因显著富集的分子功能多与转录因子相关。KEGG富集分析表明，W1vsW0、W2vsW0和W2vsW1的差异表达基因分别被注释到74、118、117条KEGG 代谢通路，共同被注释到的有71条，其中显著富集的通路有次生代谢产物的生物合成和苯丙烷生物合成等；与W1vsW0相比，W2vsW1的差异基因显著富集的KEGG 代谢通路增加了α-亚麻酸代谢通路。温度升高可促进植物的新陈代谢，有助于碳水化合物的积累，但过高的温度会降低水分的运输速率，α-亚麻酸代谢相关基因大量表达，表明α-亚麻酸可能在核桃根系应对高温胁迫中发挥重要作用。
- Abstract:
- Taking 15-year-old ‘Lyuling’ walnut trees as experimental materials，the effects of different ground temperatures(16.4 ℃，W0；21.7 ℃，W1；27.1 ℃,W2) on gene expression of walnut roots were studied by the high-through transcriptome sequencing technology，in order to explore the functional genes related to temperature regulation and explore the molecular mechanism of response of walnut roots to natural temperature regulation.The up-regulated (down-regulated) genes of W1vsW0，W2vsW0 and W2vsW1 were 430(247)，2 110(2 494) and 2 385(3 015)，respectively.GO enrichment analysis showed that differentially expressed genes of W1vsW0，W2vsW0，W2vsW1 were mainly concentrated in biological processes and molecular functions and the significantly enriched category of the three components were amino sugar catabolic process，single-organism biosynthetic process，chitin catabolic process，glucosamine-containing compound catabolic process，oxidation-reduction processand oxidoreductase activity.But the significantly enriched molecular functions category of W1vsW0 were mostly related to the metabolism of substances，while W2vsW1 were mostly related to the transcription factor.KEGG enrichment analysis illustrated that differentially expressed genes of W1vsW0，W2vsW0，W2vsW1 were annotated 74，118 and 117 KEGG pathways and there were 71 biological pathways jointly annotated，among which，the significantly enriched pathways were biosynthesis of secondary metabolites and phenylpropanoid biosynthesis.Compared with W1vsW0,the significantly enriched pathways of the differentially expressed genes of W2vsW1 increased pathway of alpha-Linolenic acid metabolism.Increased temperature could promote plant metabolism and contribute to carbohydrate accumulation.However，excessive temperature decreases the rate of water transport and a large number of genes related to the alpha-Linolenic acid metabolism acid are expressed，which suggested that alpha-Linolenic acid may play an important role in the response of walnut roots to high temperature stress.
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备注/Memo
第一作者简介：刘洋（1991-），男，河北高阳人，博士，助理研究员，现主要从事经济林栽培生理等研究工作。E-mail:1203110893@qq.com.责任作者：齐国辉（1969-），女，河北遵化人，博士，教授，现主要从事经济林栽培教学及科研等工作。E-mail:bdqgh@sina.com.基金项目：河北省科技计划资助项目（20536807D）；林业公益性行业科研专项经费资助项目（201504408）。收稿日期：2019-11-11