YANG Lijuan,GAO Wei,ZHANG Chunxiang,et al.Sensitivity of Sclerotinia sclerotiorum From Vegetable to Six Fungicides in Tianjin[J].Northern Horticulture,2020,44(09):66-72.[doi:10.11937/bfyy.20193035]
天津地区蔬菜菌核病菌对六种杀菌剂的敏感性检测
- Title:
- Sensitivity of Sclerotinia sclerotiorum From Vegetable to Six Fungicides in Tianjin
- Keywords:
- Tianjin; Sclerotinia sclerotiorum; fungicides; sensitivity
- 文献标志码:
- A
- 摘要:
- 以天津地区蔬菜菌核病菌为试材，采用菌丝生长速率法，研究了天津地区蔬菜菌核病菌对6种杀菌剂的敏感性，以期为天津地区蔬菜菌核病的防治提供指导。结果表明：氟啶胺、咪鲜胺、腐霉利、啶酰菌胺、异菌脲和多菌灵对10个菌株的EC50值分别为0.003 0~0.035 3、0.001 7~0.107 3、0.015 4~0.192 9、0.016 1~2.055 4、0.004 2~0.178 9、0.009 8~0.131 9 μg·mL-1。其中菌株XK1对氟啶胺和XK2对腐霉利的敏感性均最低，而DQZ1对这2种杀菌剂最敏感；XK2对啶酰菌胺和DQZ2对异菌脲的敏感性均最低，而XK1对这2种杀菌剂最敏感；WZ2对咪鲜胺和XK3对多菌灵的敏感性均最低，而对咪鲜胺和多菌灵最敏感的菌株分别是YLQ和WZ1。试验表明，不同菌株之间对6种杀菌剂的敏感性存在差异，部分菌株对杀菌剂的敏感性存在地区间差异。
- Abstract:
- Taking Sclerotinia sclerotiorum strains from vegetable in Tianjin as experimental materials,the sensitivity of Sclerotinia sclerotiorum to 6 fungicides were determined according to the rates of the mycelia growth of Sclerotinia sclerotiorum in media containing the fungicides,to provide theoretical guidance for controlling Sclerotinia sclerotiorum from vegetable in Tianjin.The results showed EC50 values of the 10 strains for fluazinam,prochloraz,procymidone,boscalid,iprodione and carbendazim rangedin 0.003 0-0.035 3,0.001 7-0.107 3,0.015 4-0.192 9,0.016 1-2.055 4,0.004 2-0.178 9,0.009 8-0.131 9 μg·mL-1,respectively.The sensitivity of strain XK1 to fluazinamand XK2 to procymidone were both the lowest,the strain DQZ1 were both the most sensitive to these two fungicides;the sensitivity of strain XK2 to boscalid and strain DQZ2 to iprodione were both the lowest,the strain XK1 were both the most sensitive to these two fungicides;the sensitivity of strain WZ2 to prochloraz and strain XK3 to carbendazim were both the lowest,and the most sensitive strains to prochloraz and carbendazim were strain YLQ and WZ1,respectively.These results indicated the sensitivity of 10 strains to 6 fungicides differed among strains,and that significantly difference of sensitivity of some strains were found among different areas.
参考文献/References:
[1]SAHARAN G S,MEHTA N.Sclerotinia diseases of crop plants:Biology,ecology and disease management[M].London:Springer,2008:484.[2]郑建秋,师迎春,胡铁军.蔬菜菌核病的识别与防治[J].中国蔬菜,2005,1(2):53-53.[3]崔文清,金红云,牛木森,等.保护地蔬菜菌核病的发生调查与防治[J].中国植保导刊,2009,29(2):24-26.[4]LIANG H J,DI Y L,LI J L,et al.Baseline sensitivity and control efficacy of fluazinam against Sclerotinia sclerotiorum[J].European Journal of Plant Pathology,2015,142(4):691-699.[5]WANG Y,DUAN Y B,ZHOU M G.Baseline sensitivity and efficacy of fluazinam in controlling Sclerotinia stem rot of rapeseed[J].European Journal of Plant Pathology,2016,144(2):337-343.[6]MAO X W,LI J S,CHEN Y L,et al.Resistance risk assessment for fluazinam in Sclerotinia sclerotiorum[J].Pesticide Biochemisty and Physiology,2017,144:27-35.[7]张冉.咪鲜胺对核盘菌生长和致病影响的研究[D].武汉:华中农业大学,2018.[8]曹敏娟.油菜菌核病菌对腐霉利的抗性及其机制[D].扬州:扬州大学,2008.[9]陈方新,齐永霞,高智谋.油菜菌核病菌对速克灵的抗药性风险研究[J].激光生物学报,2012,21(2):145-149.[10]潘以楼,朱桂梅,郭建,等.油菜菌核病菌对啶酰菌胺的敏感性及对不同杀菌剂敏感性的相关分析[J].西南农业学报,2012,25(2):507-512.[11]HU S,ZHANG J,ZHANG Y,et al.Baseline sensitivity and toxic actions of boscalid against,Sclerotinia sclerotiorum[J].Crop Protection,2018,110:83-90.[12]LIU X,YIN Y,YAN L,et al.Sensitivity to iprodione and boscalid of Sclerotinia sclerotiorum isolates collected from rapeseed in China[J].Pesticide Biochemistry and Physiology,2009,95(2):106-112.[13]乔昕,余洋,陈杰,等.川渝地区油菜菌核病菌对多菌灵的抗性检测及抗性机理研究[J].西南大学学报(自然科学版),2016,38(5):26-30.[14]WANG Y,HOU Y P,CHEN C J,et al.Detection of resistance in Sclerotinia sclerotiorumto carbendazim and dimethachlon in Jiangsu Province of China[J].Australasian Plant Pathology,2014,43(3):307-312.[15]徐文杰.油菜菌核病菌对多菌灵的抗药性研究[D].武汉:华中农业大学,2011.[16]乔广行,李兴红,周莹,等.北京地区不同寄主蔬菜菌核病菌抗药性比较[J].江苏农业科学,2014,42(12):155-157．[17]谭清,高熹,吴毅歆,等.氟啶胺灌药浓度和时间对大白菜根肿病防效的影响[J].农业灾害研究,2014,4(8):1-2.[18]邢益范,陈坚.22%多抗·咪鲜胺微乳剂对黄瓜白粉病的室内毒力及田间药效评价[J].现代农药,2012,11(5):54-56.[19]石凤梅.80%腐霉利可湿性粉剂对番茄灰霉病的防效研究[J].黑龙江农业科学,2014(7):68-69.[20]唐明,晋知文,谢学文,等.啶酰菌胺对黄瓜灰霉病防治效果的综合评价[J].中国蔬菜,2016(2):51-55.[21]黄海,张鑫,邹杭,等.番茄灰霉病菌室内毒力测定及药效试验[J].西北农林科技大学学报(自然科学版),2015,43(2):184-190.[22]于海龙,古瑜,韩启厚,等.多菌灵对豇豆枯萎病病原菌抑菌效果及包衣后对种子生活力的影响[J].北方园艺,2013(12):113-115.
相似文献/References:
[1]王燕,王春伟,高洁,等.不同杀菌剂及其配比对人参菌核病菌的毒力测定[J].北方园艺,2014,38(07):115.
WANG Yan,WANG Chun-wei,GAO Jie,et al.Toxicity Test of Different Fungicides and Its Mixed Preparations Against Sclerotinia schinseng[J].Northern Horticulture,2014,38(09):115.
[2]华明艳,宋兰芳,崔少杰,等.天津引种“京香”系列草莓新品种的灰色关联度分析[J].北方园艺,2017,41(16):71.[doi:10.11937/bfyy.20165178]
HUA Mingyan,SONG Lanfang,CUI Shaojie,et al.Grey Relational Analysis of ‘Jingxiang’ Series Strawberry New Varieties in Tianjin Area[J].Northern Horticulture,2017,41(09):71.[doi:10.11937/bfyy.20165178]
备注/Memo
第一作者简介：杨利娟（1988-），女，博士，助理研究员，现主要从事种苗病害检测及处理技术等研究工作。E-mail:yanglijuan1121@163.com.责任作者：王勇（1971-），女，硕士，研究员，现主要从事植物病理学等研究工作。E-mail:wangyongwb@126.com.基金项目：国家重点研发计划资助项目（2017YFD02016）；天津市科技重大专项与工程计划资助项目（16ZXHLNC00070）；天津市蔬菜现代农业产业技术体系资助项目（ITTVRS2017010）。收稿日期：2019-11-15