LI Xin,HE Lilong,ZHANG Ye,et al.Preliminary Screening and Analysis of Cadmium (Cd) Low Absorption Accumulation Chinese Cabbage Cultivars[J].Northern Horticulture,2020,44(05):21-27.[doi:10.11937/bfyy.20192729]
镉(Cd)低吸收积累大白菜品种的初步筛选与分析
- Title:
- Preliminary Screening and Analysis of Cadmium (Cd) Low Absorption Accumulation Chinese Cabbage Cultivars
- Keywords:
- Chinese cabbage; cadmium (Cd); accumulation; absorption
- 文献标志码:
- A
- 摘要:
- 以26个大白菜品种为试材,采用原子吸收分光光度法、非损伤微测技术,研究了大白菜对镉(Cd)积累以及吸收数量,以初步筛选Cd低积累低吸收大白菜品种。结果表明:大白菜品种ZBX叶片中镉积累水平较高,而JL-75叶片中镉积累水平较低。高镉积累大白菜品种ZBX根部对镉吸收的能力较高,低镉积累大白菜品种JL-75根部对镉吸收能力较低,说明根对Cd2+吸收的NMT结果与富集特征一致。线性回归分析表明,不同的大白菜品种在吸收过程中,吸收速度并不会随着时间的增加一直上升,且流速到达稳定值的时间点也不相同,由此推断,这也是导致大白菜吸收能力不同的因素之一。该研究结果对进一步挖掘大白菜调控Cd吸收以及叶片积累的作用机制具有重要意义。
- Abstract:
- To exploring the accumulation and absorption of cadmium (Cd) in Chinese cabbage,atomic absorption spectrophotometry and the Noninvasive Microtest Technology (NMT) were carried out using 26 Chinese cabbage cultivars.This study had initially screened the Chinese cabbage cultivar with the low-Cd accumulation and absorption.The results indicated that the Cd content in the leaves of Chinese cabbage ZBX was higher,while the JL-75 was lower.The root of ZBX with higher Cd accumulation showed a stronger ability of Cd absorption;and the root of the JL-75 with lower Cd accumulation showed weaker ability to absorb Cd2+,indicating that the NMT results of Cd2+ absorption were consistent with the Cd accumulation levels in Chinese cabbages.Linear regression analysis showed that,during the absorption processes in different Chinese cabbages,the absorption rate did not increase with time,and the time points was different when the flow rate reached a stable value.It was suggested that this result could also be the reason of the difference of the Cd absorption ability in Chinese cabbages.This study was significant for further exploring the mechanism of the Cd absorptionin Chinese cabbage and the Cd accumulation in the leaves.
参考文献/References:
[1]朱慧君,江婷,郭海川.重金属污染土壤修复技术研究进展[J].生物化工,2019,5(2):105-107.[2]DUZGOREN A N S.Sources and characteristics of lead pollution in the urban environment of Guangzhou[J].Sci Total Environ,2007,385(1-3):182-195.[3]HEBERER T.Occurrence,fate,and removal of pharmaceu-tical residues in the aquatic environment:A review of recent research data[J].Toxicol Lett,2002,131(1-2):5-17.[4]MEENA R A A,SATHISHKUMAR P,AMEEN F,et al.Heavy metal pollution in immobile and mobile components of lentic ecosystems-a review[J].Environ Sci Pollut Res Int,2018,25(5):4134-4148.[5]PASSARIELLO B,GIULIANO V,QUARESIMA S,et al.Evaluation of the environmental contamination at an abandoned mining site[J].Microchemical Journal,2002,73(1):245-250.[6]罗子奕.工业区场地土壤重金属污染及健康风险评价分析[J].广东化工,2019,46(8):156-157.[7]KAPLAN O,INCE M,YAMAN M.Sequential extraction of cadmium in different soil phases and plant parts from a former industrialized area[J].Environmental Chemistry Letters,2011,9(3):397-404.[8]NAWROT T,PLUSQUIN M,HOGERVORST J,et al.Environmental exposure to cadmium and risk of cancer:A prospective population-based study[J].Lancet Oncol,2006,7(2):119-126.[9]张静,赵秀侠,汪翔,等.重金属镉(Cd)胁迫对水芹生长及生理特性的影响[J].植物生理学报,2015,51(11):1969-1974.[10]HORIGUCHI H,TERANISHI H,NIIYA K,et al.Hypoproduction of erythropoietin contributes to anemia in chronic cadmium intoxication:Clinical study on Itai-itai disease in Japan[J].Arch Toxicol,1994,68(10):632-636.[11]李贵,童方平,刘振华.衡阳水口山铅锌矿区重金属污染现状的分析[J].中南林业科技大学学报,2012,32(7):105-109.[12]李国庆.从广东“镉大米”事件看我国粮食质量安全的监管[J].河南工业大学学报(社会科学版),2013,9(3):20-23.[13]李慧,刘艳,卢海威,等.湖南镉污染农田土壤钝化后两个品种水稻的生长效应[J].安全与环境学报,2016,16(6):298-302.[14]李书幻,温祝桂,陈亚茹,等.我国蔬菜重金属污染现状与对策[J].江苏农业科学,2016,44(8):231-235.[15]张帆,谢玲玲,弥宝彬,等.蔬菜对重金属镉富集研究进展[J].湖南农业科学,2017(7):127-130.[16]江海,余海娟,李亮,等.水稻核心种质的耐镉性鉴定[J].植物生理学报,2015,51(10):1617-1624.[17]龚振平.大白菜抗病和晚抽薹性状的GWAS分析及其优异资源发掘[D].北京:中国农业科学院,2016.[18]龚振平,于拴仓,张凤兰,等.大白菜感官品质评价及其与营养成分含量的关系[J].河南农业科学,2015,44(11):104-108.[19]王国泽.果蔬产品中的功能性成分[J].内蒙古农业大学学报(自然科学版),2008(1):253-256.[20]杨帅.近十年南京市郊区大白菜部分营养组分情况调查及影响因素分析[D].广州:仲恺农业工程学院,2016.[21]HE L,JING Y,SHEN J,et al.Mitochondrial pyruvate carriers prevent cadmium toxicity by sustaining the TCA cycle and glutathione synthesis[J].Plant Physiol,2019,180(1):198-211.[22]奚天雪,杨磊,袁玫,等.NaCl胁迫对甘蓝、白菜和油菜种子萌发的影响[J].种子,2016,35(6):32-35.[23]马捷.水稻(Oryza sativa)细胞壁上有机、无机硅抑制镉离子吸收的化学机制[D].武汉:华中农业大学,2016.[24]王少杰,赵楠,申泽丹,等.镉胁迫下NO对胡杨细胞Cd2+吸收调控机制的研究[J].北京林业大学学报,2015,37(6):11-16.[25]MCLAUGHLIN M J,PARKER D R,CLARKE J M.Metals and micronutrients-food safety issues[J].Field Crops Research,1999,60(1):143-163.[26]WAALKES M P.Cadmium carcinogenesis in review[J].J Inorg Biochem,2000,79(1-4):241-244.[27]YAMAGUCHI H,FUKUOKA H,ARAO T,et al.Gene expression analysis in cadmium stressed roots of a low cadmium-accumulating solanaceous plant,Solanum torvum[J].J Exp Bot,2010,61(2):423-437.[28]迟克宇,范洪黎.不同积累型苋菜(Amaranthus mangostanus L.)镉吸收转运特征差异性研究[J].植物营养与肥料学报,2016,22(6):1612-1619.[29]QUASTEL J H,STEWART C P,TUNNICLIFFE H E.On Glutathione.IV.Constitution[J].Biochem J,1923,17(4-5):586-592.[30]STEWART C P,TUNNICLIFFE H E.Glutathione:Synthesis[J].Biochem J,1925,19(2):207-217.[31]HIGGINS C F.ABC transporters:From microorganisms to man[J].Annu Rev Cell Biol,1992(8):67-113.[32]MOREL M,CROUZET J,GRAVOT A,et al.AtHMA3,a P1B-ATPase allowing Cd/Zn/Co/Pb vacuolar storage in Arabidopsis[J].Plant Physiol,2009,149(2):894-904.[33]CELLIER M,PRIVE G,BELOCHI A,et al.Nramp defines a family of membrane proteins[J].Proc Natl Acad Sci USA,1995,92(22):10089-10093.
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备注/Memo
第一作者简介:李鑫(1995-),女,山东济南人,硕士研究生,研究方向为蔬菜重金属污染防控及分子机制。E-mail:15621598391@163.com.责任作者:高建伟(1968-),男,山东汶上人,博士,研究员,现主要从事蔬菜遗传育种及栽培等研究工作。E-mail:jianweigao3@qq.com.基金项目:国家重点研发计划资助项目(2017YFD0101801);山东省农业科学院农业科技创新工程资助项目(CAAS-XTCX2018021);国家大宗蔬菜现代农业产业技术体系济南试验站建设资助项目(CARS-25-G-24);山东省现代农业产业技术体系蔬菜创新团队建设资助项目(SDAIT-02-022-04)。收稿日期:2019-09-29