MA Guanxue,ZHONG Wangao,DUAN Xinyu,et al.Rapid Propagation Technique of One-step-seedling Inducing From Young Leaves in Actinidia chinensis cv.‘Chuhong’[J].Northern Horticulture,2019,43(19):28-33.[doi:10.11937/bfyy.20184549]
“楚红”猕猴桃叶片一次成苗诱导的快繁技术
- Title:
- Rapid Propagation Technique of One-step-seedling Inducing From Young Leaves in Actinidia chinensis cv.‘Chuhong’
- 文献标志码:
- A
- 摘要:
- 以无菌萌发的“楚红”猕猴桃幼苗叶片为试材，研究了诱导培养基中植物激素的不同浓度组合对叶片不定芽直接诱导的影响，同时对不定芽所形成幼苗的生根培养基进行了优化，以期为建立猕猴桃的高效组培快繁体系提供参考依据。结果表明：猕猴桃幼嫩叶片在MS+3.0 mg?L-1 6-BA+0.3 mg?L-1 NAA培养基中培养40 d后，可从叶片直接诱导出可用于生根的不定芽，诱导率可达87.5%。不定芽诱导生根最佳培养基为1/2MS+0.7 mg?L-1 IBA+30 g?L-1蔗糖，生根率达100%，平均生根数高达26条。按该繁殖方法，1粒猕猴桃种子，1年后可得到800万株幼苗，且通过切片观察，不定芽发生途径为体细胞胚发生途径。
- Abstract:
- In order to establish an efficient tissue culture and rapid propagation system for kiwifruit,the young leaves of seedling derived from the aseptic germination of kiwifruit seed of Actinidia chinensis cv.‘Chuhong’ was used as material,the effect of the various concentration combination of plant hormone added to inducing medium on the induce of adventitious bud directly were studied;and the rooting medium for plantlet derived adventitious bud was also optimized.The results showed that in the inducing medium,with 3.0 mg?L-1 6-BA+0.3 mg?L-1 NAA as plant hormone combination,the adventitious bud which could be used for rooting from the young leaves could be induced directly within 40 days,and the adventitious bud inducing frequency could arrived 87.5%.And the optimized rooting medium for plantlet derived from adventitious bud was 1/2MS+0.7 mg?L-1 IBA+30 g?L-1 sucrose,the rooting rate could arrived 100% with the average root number up to 26 per plant.One kiwifruit seed could propagated about 8 million shares seedlings in one year basing the rapid propagation technique established in present experiment，and most of the adventitious bud was formed through the somatic embryogenesis pathway via observation of histological sections.
参考文献/References:
[1]吴晓梅.猕猴桃遗传育种及其产业化进展[J].福建林业科技,2010,37(2):174-178.[2]于红梅,赵密珍,钱亚明,等.海沃德猕猴桃带芽茎段的组织培养快繁技术[J].江苏农业科学,2014,42(11):78-79.[3]赵金梅,高贵田,薛敏,等.不同品种猕猴桃果实的品质及抗氧化活性[J].食品科学,2014,35(9):118-122.[4]贺志智,张生浩,刘明星.湖南省永顺县猕猴桃种植的气候条件分析[J].北京农业,2015(9):229-230.[5]严姜黎,张翼,邢梅,等.红肉猕猴桃离体快速繁殖技术研究[J].华中农业大学学报,2008,27(1):101-104.[6]王文平,王明力,周文美.猕猴桃果茶加工工艺的研究[J].贵州工业大学学报（自然科学版）,2005,34(4):31-33.[7]陈红惠.猕猴桃籽成分分析及活性油脂提取工艺的研究[J].文山学院学报,2005,18(4):326-328.[8]罗强,刘建林.攀西地区野生猕猴桃资源及分布概况[J].江苏农业科学,2009(4):373-375.[9]黄宏文,王圣梅,张忠慧,等.猕猴桃高效栽培[M].北京:金盾出版社,2006.[10]杨海健,伊洪伟,韩国辉,等.重庆大巴山区野生猕猴桃资源调查和遗传多样性分析[J].植物遗传资源学报,2018,19(2):187-193.[11]LIU Y,HUANG H.Genetic variation and natural hybridization among sympatric Actinidia,species and the implications for introgression breeding of kiwifruit[J].Tree Genetics & Genomes,2010,6(5):801-813.[12]LI J,HUANG H,TAO S.Molecular phylogeny and in frageneric classification of Actinidia(Actinidiaceae)[J].Systematic Botany,2002,27(2):408-415.[13]CHAT J,JAUREGUI B,PETIT R J,et al.Reticulate evolution in kiwifruit(Actinidia,Actinidiaceae) identified by comparing their maternal and paternal phylogenies[J].American Journal of Botany,2004,91(5):736-747.[14]PRADO M J,GONZALEZ M V,ROMO S,et al.Adventitious plant regeneration on leaf explants from adult male kiwifruit and AFLP analysis of genetic variation[J].Plant Cell Tissue and Organ Culture,2007,88(1):1-10.[15]于海燕,陈刚,建德锋.毛花猕猴桃组培苗增殖扩繁培养基筛选试验[J].北方园艺,2013(4):111-113.[16]文国琴,何震.红阳猕猴桃茎段愈伤组织诱导成苗技术[J].福建林业科技,2004,31(4):78-79.[17]赵许朋,罗克明,周月,等.‘红阳’猕猴桃叶盘高频直接再生体系的建立[J].生物工程学报,2013,29(11):1599-1606.[18]隆前进,吴延军,谢鸣.‘红阳’猕猴桃叶片和带芽茎段的组织培养快繁技术[J].浙江农业学报,2010,22(4):429-432.[19]姜维梅,李凤玉.大籽猕猴桃(Actinidia macrosperma)离体再生系统的建立[J].浙江大学学报(农业与生命科学版),2003,29(3):295-299.[20]刘坤,李勇,吴易雄,等.对萼猕猴桃无菌离体再生体系研究[J].园艺与种苗,2015(10):35-38.[21]OBAID A A.Seeds germination and growth adventitious budding from shoots and callus of kiwifruit(Actinidia deliciosa) in vitro[J].Diyala Agricultural Sciences Journal,2012,4(1):284-295.[22]WU Y J,XIE M,LONG Q J.In vitro organogenesis and plant regeneration from leaves of Actinidia eriantha Benth.cv White(kiwifruit)[J].New Zealand Journal of Crop & Horticultural Science,2011,39(4):231-240.[23]谢志兵,鲁旭东.猕猴桃组织培养中适宜激素组合的筛选[J].北方果树,2003(3):7-8.[24]SEELYE J,MADDOCKS D J,BURGE G K,et al.Shoot regeneration from leaf discs of Limonium perigrinum using thidiazuron[J].New Zealand Journal of Experimental Agriculture,1994,22(1):23-29.[25]陈华,李平,刘晶,等.药蒲公英再生体系的建立和优化[J].生物工程学报,2005,21(2):244-249.[26]THORPE T A.In vitro,organogenesis and somatic embryogenesis:Physiological and biochemical aspects[M]//ROUBELAKIS-ANGELAKIS K A,TRAN T V K.Morphogenesis in plants:Molecular Approaches.New York,Plenum Press,1993.[27]HE D G,YANG Y M,BERTRAM J,et al.The histological development of the regenerative tissue derived from cultured immature embryos of wheat(Triticum aestivum L.)[J].Plant Science,1990,68(1):103-111.[28]OVE〖AKCˇ〗KA M,BOBK M,BLEHOV A,et al.Papaver somniferum regeneration by somatic embryogenesis and shoot organogenesis[J].Biologia Plantarum,1997,40:321-328.[29]CHATURVEDI H C,MITRA G C.A Shift in morphogenetic pattern in citrus callus tissue during prolonged culture[J].Annals of Botany,1975,39(4):683-687.[30]KAMADA H,HARADA H.Studies on the Organogenesis in carrot tissue cultures I:Effects of growth regulators on somatic embryogenesis and root formation[J].Zeitschrift Für Pflanzenphysiologie,1979,91(3):255-266.[31]CHHABRA G,CHAUDHARY D,VARMA M,et al.TDZ-induced direct shoot organogenesis and somatic embryogenesis on cotyledonary node explants of lentil(Lens culinaris Medik.)[J].Physiology & Molecular Biology of Plants An International Journal of Functional Plant Biology,2008,14(4):347-353.[32]GUAN Y,LI S G,FAN X F,et al.Application of somatic embryogenesis in woody plants[J].Frontiers in Plant Science,2016(7):938-949.[33]马有会.猕猴桃体细胞胚状体的诱导及植株再生[J].北方园艺,1993(6):6-8.
备注/Memo
第一作者简介：马关雪（1994-），女，硕士研究生，研究方向为植物资源与植物遗传。E-mail：as13888108455@qq.com.责任作者：张云峰（1964-），男，硕士，教授，硕士生导师，现主要从事植物资源与植物遗传等研究工作。E-mail：zhyunfen001@163.com.基金项目：国家自然科学基金资助项目(30660075，31360263)；云南省科技厅社会发展科技计划基础研究面上资助项目(2009CD052)；云南省基础研究计划资助项目(2011FA016)。收稿日期：2019-03-20