XIE Zhongli,LI Dan,YU Xiangjun,et al.Building and Optimizing the Tissue Culture System of Sequoia sempervirens[J].Northern Horticulture,2019,43(06):96-101.[doi:10.11937/bfyy.20183728]
北美红杉组培快繁体系的建立与优化
- Title:
- Building and Optimizing the Tissue Culture System of Sequoia sempervirens
- 文献标志码:
- A
- 摘要:
- 以北美红杉1年生带芽茎段为外植体，通过基本培养基筛选和不同植物生长调节剂水平对比试验得到北美红杉组织培养的不定芽诱导、增殖、生根等生长阶段的优化配比培养基，以期建立北美红杉高效的快繁体系。结果表明：确定最佳消毒方法为75%乙醇消毒30 s，无菌水冲洗2次，再用0.1%氯化汞消毒处理10 min，无菌水冲洗4次；最佳不定芽诱导培养基配方为MS+6-BA 1.0 mg?L-1+NAA 0.10 mg?L-1+蔗糖30 g?L-1+琼脂6 g?L-1，不定芽诱导效果最好，诱导芽率高达2 153.3%，且芽生长健壮；最佳不定芽增殖培养基配方为MS+6-BA 1.0 mg?L-1+NAA 0.10 mg?L-1+蔗糖30 g?L-1+琼脂6 g?L-1，增殖系数高达15.8；最佳生根培养基配方为1/2MS+KT 1.5 mg?L-1+IBA 1.0 mg?L-1+蔗糖30 g?L-1+琼脂6 g?L-1，其生根率达90.0%，根多且粗长，植株高大健壮；最佳移栽基质为V炉渣∶V原土∶V腐殖土=1∶1∶1，植株成活率达91.7%。
- Abstract:
- One-year-old Sequoia sempervirens stem with buds were chosen as explants,by the basic medium and different plant growth regulator levels of contrast test to optimize the ratio of tissue culture of S.sempervirens callus induction,obtaining optimized culture medium of adventitious buds induction,proliferation,rooting and other several growth stages of the S.sempervirens tissue culture.The establishment of S.sempervirens tissue culture system was to improve the reproduction capacity and increase the S.sempervirens,and provide a certain theoretical basis and scientific basis for the rapid tissue culture of S.sempervirens.The results indicated that the best disinfection method was to use 75% alcohol to disinfect for 30 seconds,rinse with sterile water twice,disinfect with 0.1% mercury bichloride for 10 minutes,and rinse with sterile water four times.The optimum medium for the adventitious shoot induction was MS+6-BA 1.0 mg?L-1+NAA 0.10 mg?L-1+sucrose 30 g?L-1+agar 6.0 g?L-1,the induction efficiency of regenerated buds was the best,and the induced buds rate was as high as 2 153.3%.The formula for the optimal adventitious shoot proliferation medium was MS+6-BA 1.0 mg?L-1+NAA 0.10 mg?L-1+sucrose 30 g?L-1+agar 6.0 g?L-1,the proliferative coefficient of adventitious shoots was as high as 15.8.The optimum culture medium for rooting induction was 1/2 MS+KT 1.5 mg?L-1+IBA 1.0 mg?L-1+sucrose 30 g?L-1+agar 6.0 g?L-1,the rooting rate was 90.0%,more root and longer length,and the plant was tall and strong.The suitable transplanting substrate was Vscoria∶Vraw soil∶Vhumus=1∶1∶1 and the survival rate of the plantlets was 91.7%.
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备注/Memo
第一作者简介：谢忠利（1993-），男，硕士研究生,研究方向为林木遗传改良与繁育技术。E-mail:1632308626@qq.com.责任作者：李旦（1985-），女，博士，副研究员,研究方向为林木遗传育种与生物技术。E-mail：lidan_163@163.com.基金项目：国家自然科学基金资助项目（31460205）；云南省环境保护专项资金资助项目（2014BI0011）；西南林业大学大学生创新基金资助项目（C17074）；西南林业大学云南省高校林木遗传改良与繁育重点实验室开放基金资助项目（YNGBS201701）。收稿日期：2018-12-03