LI Min,LI Enmao,AN Xiuhong,et al.Effect of Amino Acid Selenium Foliar Fertilizer on Apple Resistance to Apple Ring Blight[J].Northern Horticulture,2019,43(10):67-71.[doi:10.11937/bfyy.20183438]
氨基酸硒叶面肥提高苹果果实对轮纹病抗性作用
- Title:
- Effect of Amino Acid Selenium Foliar Fertilizer on Apple Resistance to Apple Ring Blight
- 文献标志码:
- A
- 摘要:
- 为探索生长期喷施氨基酸硒叶面肥对苹果果实轮纹病的防治作用,以盛果期“长富2号”苹果为试材,通过田间连续喷施氨基酸硒叶面肥,调查苹果果实轮纹病的发病情况并测定果实相关抗性酶活性,明确氨基酸硒叶面肥对苹果轮纹病的防治作用。结果表明:单独喷施氨基酸硒叶面肥防治效果约20%,但与70%甲基硫菌灵配合使用的联合防治效果可达90%以上,高于单独使用杀菌剂防效,防效提高约15个百分点;同时,施用氨基酸硒叶面肥后,果实内脯氨酸含量(Pro)以及超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性明显升高,丙二醛(MDA)含量降低。综上所述,氨基酸硒叶面肥虽不能单独用于控制病害的发生和流行,但配合杀菌剂使用时,能明显提高杀菌剂的防治效果;并且氨基酸硒叶面肥可通过提高苹果脯氨酸含量和保护酶活性,同时降低膜脂过氧化产物MDA的积累来提高苹果对果实轮纹病的抗性。
- Abstract:
- To assay the control efficiency of amino acid selenium foliar fertilizer to apple ring blight,apple (Malus domestica) ‘Nagafu 2’ was used to investigate the control efficiency of spaying amino acid selenium against apple ring rot.The results showed that the control efficiency was 20% against apple ring rot by spaying amino acid selenium at growing season of apple.However,the control efficiency of amino acid selenium was up to 90% when combined with thiophanate-methyl.This value was increased about 15% than that one of treated with thiophanate-methyl alone.More importantly,the activities of superoxide dismutase (SOD),peroxidase (POD),and catalase (CAT) as well as proline (Pro) content were significantly enhanced in apple fruits treated with amino acid selenium alone or in combination with thiophanate-methyl.The malondialdehyde (MDA) content was declined at the same time.In conclusion,the spaying amino acid selenium at growing season could enhance the control efficiency of fungicide.Furthermore,the amino acid selenium could increase the activities of defensive enzymes and reduce the accumulation of MDA.
参考文献/References:
[1]李保华,王彩霞,董向丽.我国苹果主要病害研究进展与病害防治中的问题[J].植物保护,2013,39(5):46-54.[2]国立耘,李金云,李保华,等.中国苹果枝干轮纹病发生和防治情况[J].植物保护,2009,35(4):120-123.[3]雍道敬,王彩霞,李桂舫,等.内生放线菌A-1对苹果果实轮纹病的防效及防御性酶活性的影响[J].植物保护学报,2014,41(3):335-341.[4]于春蕾,孟璐璐,练森,等.BHT对苹果果实轮纹病的防效及防御酶活性的影响[J].植物生理学报,2018(5):819-826.[5]彭耀湘,陈正法.硒的生理功能及富硒水果的开发利用[J].农业现代化研究,2007(3):381-384.[6]郭孝.硒钴在“土-草-饲-畜链”(SPFAC)中对草畜生长与营养的调控[D].郑州:河南农业大学,2010.[7]明佳佳.硒增强油菜对菌核病抗性及其生理机制[D].武汉:华中农业大学,2016.[8]吴玉星,周宗山,王孝娣,等.生长期喷施氨基酸硒叶面肥对苹果树腐烂病的控制作用研究[J].中国果树,2012(5):51-53.[9]李敏,厉恩茂,李壮,等.氨基酸硒叶面肥提高果树抗病性作用研究[J].中国南方果树,2013,42(5):93-94,97.[10]高俊凤.植物生理学实验指导[M].北京:高等教育出版社,2006.[11]张志良,瞿伟菁.植物生理学实验指导[M].3版.北京:高等教育出版社,2003.[12]彭西,崔恒敏.硒对免疫功能影响的研究进展[J].中国兽医科学,2010,40(8):867-872.[13]李明,郭孝.硒钴肥基施对增强苜蓿防病能力的影响[J].家畜生态学报,2011,32(2):36-40.[14]吴洁.硒调控果蔬衰老与抗病性的作用机制研究[D].合肥:安徽农业大学,2016.[15]薛泰麟,侯少范,谭见安,等.硒在高等植物体内的抗氧化作用Ⅰ.硒对过氧化作用的抑制效应及酶促机制的探讨[J].科学通报,1993(3):274-277.[16]薛泰麟,侯少范,谭见安,等.硒在高等植物体内的抗氧化作用Ⅱ.非酶机制的探讨[J].科学通报,1993(4):356-358.[17]赵林川,俞炳杲.硒对玉米叶片衰老的调节作用[J].南京农业大学学报,1996(1):22-25.[18]ZHANG Q,YONG D,ZHANG Y,et al.Streptomyces rochei A-1 induces resistance and defense-related responses against Botryosphaeria dothidea in apple fruit during storage[J].Postharvest Biology & Technology,2016,115:30-37.[19]杨舒贻,陈晓阳,惠文凯,等.逆境胁迫下植物抗氧化酶系统响应研究进展[J].福建农林大学学报(自然科学版),2016,45(5):481-489.[20]RAZAKA A,ELTANTAWY H,ELSHEIKHH H,et al.Influence of selenium on the efficiency of fungicide action against certain fungi[J].Biological Trace Element Research,1991,28(1):47-56.[21]王海波,王孝娣,姚秀业,等.氨基酸硒叶面肥在玫瑰香葡萄上的应用效果[J].中外葡萄与葡萄酒,2011(5):47-49.
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备注/Memo
第一作者简介:李敏(1978-),女,硕士,副研究员,现主要从事苹果抗旱生理等研究工作。E-mail:liminhappy-2003@163.com.责任作者:程存刚(1969-),男,博士,研究员,现主要从事苹果栽培生理等研究工作。E-mail:ccungang2003@163.com.基金项目:国家苹果产业技术体系资助项目(nycytx 28);重大自然灾害监测预警与防范重点专项资助项目(2017YFC1502805)。收稿日期:2019-01-23