LI Meng,LIU Songhu,ZHANG Yan,et al.Effects of Exogenous 24-Epibrassinolide on Photosynthetic Characteristics and Quality of Brassica pekinensis Under Weak Light Stress[J].Northern Horticulture,2019,43(10):72-79.[doi:10.11937/bfyy.20183249]
外源24-表油菜素内酯对弱光胁迫下黄心菜光合特性和品质的影响
- Title:
- Effects of Exogenous 24-Epibrassinolide on Photosynthetic Characteristics and Quality of Brassica pekinensis Under Weak Light Stress
- 关键词:
- 24-表油菜素内酯（EBR）; 弱光胁迫; 黄心菜; 光合特性; 品质
- Keywords:
- 24-epibrassinolide(EBR); weak light stress; Brassica pekinensis; photosynthetic characteristics; quality
- 文献标志码:
- A
- 摘要:
- 以营养液栽培的黄心菜品种‘淮南F1’为试验材料，对比分析弱光胁迫下喷施外源24-表油菜素内酯（24-epibrassinolide，EBR）黄心菜的生长、光合特性、抗氧化代谢和品质等指标。结果表明：弱光胁迫可显著降低黄心菜植株的株高、根长、叶面积、根体积和干鲜质量，而叶面喷施EBR可有效缓解弱光胁迫对其生长的抑制作用。施用EBR后，黄心菜叶片净光合速率（Pn）、气孔导度（Gs）和蒸腾速率（Tr）分别显著升高42.4%、107%和75.3%，叶绿素荧光参数Fv/Fm、ФPSⅡ和ETR分别升高31.0%、34.4%和15.7%，叶绿素总量下降14.6%。外源EBR显著提高了弱光胁迫下黄心菜叶片抗氧化酶活性，与弱光胁迫相比，超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）和抗坏血酸氧化酶（APX）的活性分别增加了36.7%、51.8%、92.7%和51.2%，并降低了超氧阴离子（O?[TX-*4]2）产生速率和丙二醛（MDA）含量。外源EBR显著提高了弱光胁迫下黄心菜植株维生素C、可溶性糖、可溶性蛋白质含量，降低了可滴定酸和硝酸盐含量，有效改善了黄心菜营养指标。可知，EBR可增强保护酶系统的活性，从而缓解弱光胁迫对黄心菜生长和光合作用的抑制，提高弱光胁迫环境条件下黄心菜的品质。
- Abstract:
- The present study aimed to investigate the effect of exogenous EBR on Brassica pekinensis cultivar (‘Huainan F1’) on growth,photosynthetic,antioxidant metabolism and quality under weak light stress by hydroponic culture.The results showed that the plant height,root length,leaf area,root volume,fresh and dry weight of Brassica pekinensis were significantly inhibited by weak light stress.However,leaf-applied EBR treatment alleviated the growth inhibition in Brassica pekinensis caused by weak light stress.Compared with weak light stress,the application of EBR remarkably increased the net photosynthetic rate (Pn),stomatal conductance (Gs) and transpiration rate (Tr) by 42.4%,107% and 75.3%，respectively.In addition,the level of Fv/Fm,ФPSⅡ and ETR significantly increased by 31.0%,34.4% and 15.7% by BR treatment under weak light stress,whereas,chlorophyll content was decreased by 14.6%.Exogenous EBR also enhanced the activities of SOD,POD,CAT and APX by 36.7%,51.8%,92.7% and 51.2%,resulting in a significant decrease of O?[TX-*4]2 and MDA contents as compared with the stress condition.In the presence of EBR,the contents of vitamin C,soluble sugar and soluble protein increased significantly,while he contents of nitrate and organic acid remarkably decreased in response to weak light stress.The results showed that exogenous EBR effectively improved the activities of antioxidant enzymes,thereby alleviating the inhibition of growth and photosynthesis,and eventually promoted the quality of Brassica pekinensis under weak light stress.
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备注/Memo
第一作者简介：李蒙（1989-），男，硕士，助教，现主要从事设施园艺与无土栽培等研究工作。E-mail：limengnlfd@163.com.责任作者：张琰（1968-），男，硕士，教授，现主要从事木本油料良种繁育及优质栽培等研究工作。E-mail：zhy8010878@163.com.基金项目：河南省科技攻关计划资助项目（172102110263）；河南省科教发展资助项目（16A210054）；河南省基础与前沿研究计划资助项目（162300410181）。收稿日期：2019-01-23