SHEN Li,ZHANG Xing,ZHANG Jianxia.Identification of Alkali Resistance of Different Grape Stock Varieties (Strains)[J].Northern Horticulture,2019,43(05):45-55.[doi:10.11937/bfyy.20182198]
不同葡萄砧木品种（系）的耐碱性评价
- Title:
- Identification of Alkali Resistance of Different Grape Stock Varieties (Strains)
- Keywords:
- grape; rootstock; alkali resistance; identification
- 文献标志码:
- A
- 摘要:
- 以25个葡萄砧木品种或株系和1个栽培品种“红地球”的盆栽植株为试材，通过浇灌浓度为20%饱和石灰水的方法模拟碱胁迫，在胁迫5、15、30、42 d后，分别调查叶片的碱害指数并测定叶片相对电导率、叶绿素、丙二醛和游离脯氨酸含量，利用隶属函数法综合评价其耐碱性。同时，测定了相应土样中有效态Ca2+、Mg2+和Fe2+含量及土壤pH，旨在揭示葡萄碱害黄化与土壤中3种离子及pH的关系。结果表明：在碱胁迫下，葡萄叶片的相对电导率及丙二醛、游离脯氨酸含量均升高；‘5BB’等11个品种（株系）的叶绿素总量先上升后下降，‘SO4’等15个品种（株系）则为先下降后上升再下降。表现高耐碱的是‘5BB’‘YT32’‘YT48’‘YH58’；耐碱的是‘3309’‘SO4’‘225Ru’‘5C’“河岸（♀）”‘1103P’‘188-08’“河岸-2”‘00-1-5’‘00-1-10’‘6-12-4’‘6-12-6’“河山-1”和‘YH39’；中等耐碱的是‘110R’‘101-14’“抗砧3号”和‘6-12-1’；不耐碱的是“贝达”“北醇”和“红地球”；极不耐碱的是“燕山-1”。土壤中有效态Ca2+、Mg2+和Fe2+离子含量为波动变化，处理后第42天与对照相比，土壤有效态Ca2+含量升高，有效态Mg2+含量变化不大，有效态Fe2+含量降低；处理组土壤pH达到8.89，比对照（7.55）高出1.34。
- Abstract:
- The alkali resistance of 25 grape rootstock varieties or strains and one cultivar ‘Red Globe’ potted plants were identified by pouring 20% saturated lime water.After 5，15，30 and 42 days of alkali stress，the alkali injury index of grape leaves was investigated，and the relative conductivity，the contents of chlorophyll，MDA as well as free proline were determined.The alkali resistance of the test materials was comprehensively evaluated by the membership function method.Meantime，the changes of available Ca2+，Mg2+ and Fe2+ content as well as pH in soil were determined.The results showed that the relative conductivity(REC)，the contents of malondialdehyde (MDA) and free proline (Pro) all increased in grape leaves under alkali stress；the contents of chlorophyll (Chl) of 11 varieties or strains such as ‘5BB’‘Heshan-1’ and ‘YH58’ first increased and then decreased，the remaining 15 varieties or strains such as ‘SO4’ first decreased and then increased，finally decreased again.‘5BB’‘YT32’‘YT48’ and ‘YH58’ belong to high resistance type；‘3309’‘SO4’‘225Ru’‘5C’，V.Riparia(♀)，‘1103P’‘188-08’，V.riparia Mcadams，‘00-1-5’‘00-1-10’‘6-12-4’‘6-12-6’‘Heshan-1’ and ‘YH39’ belong to resistance type；‘110R’‘101-14’‘Kangzhen-3’ and ‘6-12-1’ belong to middle resistance type；‘Beta’‘Beichun’ and ‘Red Globe’ belong to susceptible type；‘Yanshan-1’ belonged to high susceptible type.The contents of available ions of calcium，magnesium and iron in the soil are disordered，and they are not simply increased or decreased but fluctuate.On 42 days after alkali stress，compared with the control (without alkali stress)，the content of available calcium ions in soil increased，the content of available magnesium changed little，the content of available iron decreased.The soil pH in the treatment group reached 8.89，which was 1.34 higher than that of the control (7.55).
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备注/Memo
第一作者简介：沈莉（1994-），女，硕士研究生，研究方向为葡萄种质资源与生物技术。E-mail：835273863@qq.com.责任作者：张剑侠（1964-），男，博士，教授，博士生导师，研究方向为葡萄种质资源与生物技术。E-mail：zhangjx666@126.com.基金项目：国家科技支撑计划子课题资助项目（2013BAD02B04-06）；公益性行业（农业）科研专项子课题资助项目（201203075-08）。收稿日期：2018-08-08