HUANG Chunyan,SHAN Hongtao,WAN Luzhang,et al.Polyphsic Taxonomy of Different Strains of Flammulina velutipes[J].Northern Horticulture,2019,43(05):142-148.[doi:10.11937/bfyy.20182137]
不同金针菇菌株多相分类研究
- Title:
- Polyphsic Taxonomy of Different Strains of Flammulina velutipes
- Keywords:
- Flammulina velutipes; strain; polyphsic taxonomy
- 文献标志码:
- A
- 摘要:
- 利用菌丝拮抗反应、酯酶同工酶以及ISSR分子标记对35个金针菇菌株进行多相分类研究，建立了遗传相似性聚类分析图。结果表明：拮抗反应与ISSR分析结果较为一致，而酯酶同工酶与拮抗反应、ISSR分析结果差异较大，ISSR聚类分析结果更为精确。根据ISSR聚类分析，当相似系数为0.86时，可将供试菌株分为7组，其中黄色金针菇的遗传多样性要高于白色金针菇。
- Abstract:
- Polyphsic taxonomy of 35 Flammulina velutipes strains was studied by mycelial antagonistic reaction,esterase isozyme and ISSR molecular markers.A genetic similarity clustering analysis map was established.The results showed that the results of antagonistic reaction and ISSR analysis were more consistent,but the results of esterase isozyme were significantly different from antagonistic reaction and ISSR analysis,and the results of ISSR cluster analysis were more accurate.According to the ISSR cluster analysis,when the similarity coefficient is 0.86,the tested strains can be divided into 7 groups,and the genetic diversity of the yellow strains is higher than that of the white strains.
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备注/Memo
第一作者简介：黄春燕（1973-），女，硕士，副研究员，现主要从事食用菌栽培及育种等研究工作。E-mail:1326468668@sina.com.责任作者：宫志远（1964-），男，本科，研究员，现主要从事食用菌育种及栽培等研究工作。E-mail:sdgzy2656@126.com.基金项目：山东省重点研发计划资助项目（2017GNC10127）；山东省重大科技创新工程资助项目(2018CXGC0202）；山东省泰山产业领军人才工程资助项目（LJNY201701）；山东省农业科学院农业科技创新工程资助项目（CXGC2017A01）。收稿日期：2018-08-01